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Literature summary extracted from

  • Qiao, Y.; Peng, Q.; Yan, J.; Wang, H.; Ding, H.; Shi, B.
    Gene cloning and enzymatic characterization of alkali-tolerant type I pullulanase from Exiguobacterium acetylicum (2015), Lett. Appl. Microbiol., 60, 52-59.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.41 expression in Escherichia coli Exiguobacterium acetylicum
3.2.1.142 expression in Escherichia coli Exiguobacterium acetylicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.41 2-mercaptoethanol 5 mM, 51% residual activity Exiguobacterium acetylicum
3.2.1.41 Cu2+ 5 mM, 67% residual activity Exiguobacterium acetylicum
3.2.1.41 EDTA 5 mM, 72% residual activity Exiguobacterium acetylicum
3.2.1.41 Mg2+ 5 mM, 90% residual activity Exiguobacterium acetylicum
3.2.1.41 SDS 5 mM, 46% residual activity Exiguobacterium acetylicum
3.2.1.142 2-mercaptoethanol 5 mM, 51% residual activity Exiguobacterium acetylicum
3.2.1.142 Cu2+ 5 mM, 67% residual activity Exiguobacterium acetylicum
3.2.1.142 EDTA 5 mM, 72% residual activity Exiguobacterium acetylicum
3.2.1.142 Mg2+ 5 mM, 90% residual activity Exiguobacterium acetylicum
3.2.1.142 SDS 5 mM, 46% residual activity Exiguobacterium acetylicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.41 additional information
-
 pullulan Km value 0.12 mg/ml, pH 6.0, 50°C Exiguobacterium acetylicum
3.2.1.41 additional information
-
 soluble starch Km value 0.69 mg/ml,pH 6.0, 50°C Exiguobacterium acetylicum
3.2.1.142 additional information
-
 pullulan Km value 0.12 mg/ml, pH 6.0, 50°C Exiguobacterium acetylicum
3.2.1.142 additional information
-
 soluble starch Km value 0.69 mg/ml,pH 6.0, 50°C Exiguobacterium acetylicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.41 Co2+ 5 mM, 198% of initial activity Exiguobacterium acetylicum
3.2.1.41 Fe2+ 5 mM, 121% of initial activity Exiguobacterium acetylicum
3.2.1.41 Mn2+ 5 mM, 136% of initial activity Exiguobacterium acetylicum
3.2.1.142 Co2+ 5 mM, 198% of initial activity Exiguobacterium acetylicum
3.2.1.142 Fe2+ 5 mM, 121% of initial activity Exiguobacterium acetylicum
3.2.1.142 Mn2+ 5 mM, 136% of initial activity Exiguobacterium acetylicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 95800
-
-
 Exiguobacterium acetylicum
3.2.1.41 100000
-
 PAGE Exiguobacterium acetylicum
3.2.1.142 95800
-
-
 Exiguobacterium acetylicum
3.2.1.142 100000
-
 PAGE Exiguobacterium acetylicum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Exiguobacterium acetylicum I3WU34
-
-
3.2.1.41 Exiguobacterium acetylicum YH5 I3WU34
-
-
3.2.1.142 Exiguobacterium acetylicum I3WU34
-
-
3.2.1.142 Exiguobacterium acetylicum YH5 I3WU34
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O 6.0% of the activity with pullulan Exiguobacterium acetylicum maltotriose + maltose + maltooligosaccharides
-
 ?
3.2.1.41 amylopectin + H2O 6.0% of the activity with pullulan Exiguobacterium acetylicum YH5 maltotriose + maltose + maltooligosaccharides
-
 ?
3.2.1.41 beta-limited dextrin + H2O 19.7% of the activity with pullulan Exiguobacterium acetylicum maltotriose + maltose + maltooligosaccharides
-
 ?
3.2.1.41 beta-limited dextrin + H2O 19.7% of the activity with pullulan Exiguobacterium acetylicum YH5 maltotriose + maltose + maltooligosaccharides
-
 ?
3.2.1.41 glycogen + H2O 6.1% of the activity with pullulan Exiguobacterium acetylicum maltotriose + maltose + maltooligosaccharides
-
 ?
3.2.1.41 glycogen + H2O 6.1% of the activity with pullulan Exiguobacterium acetylicum YH5 maltotriose + maltose + maltooligosaccharides
-
 ?
3.2.1.41 additional information no substrates: maltotriose, maltose Exiguobacterium acetylicum ?
-
 ?
3.2.1.41 additional information no substrates: maltotriose, maltose Exiguobacterium acetylicum YH5 ?
-
 ?
3.2.1.41 pullulan + H2O
-
 Exiguobacterium acetylicum maltotriose + ? complete conversion to maltotriose ?
3.2.1.41 pullulan + H2O
-
 Exiguobacterium acetylicum YH5 maltotriose + ? complete conversion to maltotriose ?
3.2.1.41 soluble starch + H2O 12.8% of the activity with pullulan Exiguobacterium acetylicum maltotriose + maltose + maltooligosaccharides
-
 ?
3.2.1.142 amylopectin + H2O 6.0% of the activity with pullulan Exiguobacterium acetylicum maltotriose + maltose + maltooligosaccharides
-
 ?
3.2.1.142 beta-limited dextrin + H2O 19.7% of the activity with pullulan Exiguobacterium acetylicum maltotriose + maltose + maltooligosaccharides
-
 ?
3.2.1.142 glycogen + H2O 6.1% of the activity with pullulan Exiguobacterium acetylicum maltotriose + maltose + maltooligosaccharides
-
 ?
3.2.1.142 additional information no substrates: maltotriose, maltose. the enzyme is strictly restricted to hydrolysis of 1->6-linked glucosidic linkages. It also acts as type I pullulanase, EC 3.2.1.41 Exiguobacterium acetylicum ?
-
 ?
3.2.1.142 pullulan + H2O
-
 Exiguobacterium acetylicum maltotriose + ? complete conversion to maltotriose ?
3.2.1.142 soluble starch + H2O 12.8% of the activity with pullulan Exiguobacterium acetylicum maltotriose + maltose + maltooligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.41 monomer 1 * 100000, SDS-PAGE, 1 * 95800, calculated Exiguobacterium acetylicum
3.2.1.142 monomer 1 * 100000, SDS-PAGE, 1 * 95800, calculated Exiguobacterium acetylicum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 Pul3YH5
-
 Exiguobacterium acetylicum
3.2.1.142 Pul3YH5
-
 Exiguobacterium acetylicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 50
-
-
 Exiguobacterium acetylicum
3.2.1.142 50
-
-
 Exiguobacterium acetylicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.41 50
-
 pH 5-8, 10 min, more than 70% of maximum activity Exiguobacterium acetylicum
3.2.1.142 50
-
 pH 5-8, 10 min, more than 70% of maximum activity Exiguobacterium acetylicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 6
-
-
 Exiguobacterium acetylicum
3.2.1.142 6
-
-
 Exiguobacterium acetylicum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.41 4 10 30 min, more than 93% residual activity Exiguobacterium acetylicum
3.2.1.142 4 10 30 min, more than 93% residual activity Exiguobacterium acetylicum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.41 Exiguobacterium acetylicum calculated
-
 6.5
3.2.1.142 Exiguobacterium acetylicum calculated
-
 6.5