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Literature summary extracted from

  • Borzecka, W.; Lavandera, I.; Gotor, V.
    Synthesis of enantiopure fluorohydrins using alcohol dehydrogenases at high substrate concentrations (2013), J. Org. Chem., 78, 7312-7317.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 expression in Escherichia coli Rhodococcus ruber
1.1.1.2 expression in Escherichia coli Levilactobacillus brevis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.1 propan-2-ol 10% v/v, enzyme catalyzes reduction of fluorinated ketones without addition of NADH Rhodococcus ruber

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Rhodococcus ruber Q8KLT9
-
-
1.1.1.2 Levilactobacillus brevis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 2,2,2-trifluoro-1-phenylethanone + propan-2-ol in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH Rhodococcus ruber (1R)-2,2,2-trifluoro-1-phenylethanol + acetone 99% conversion, 99% enantiomeric excess ?
1.1.1.1 2,2-difluoro-1-phenylethanone + propan-2-ol in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH Rhodococcus ruber (1R)-2,2-difluoro-1-phenylethanol + acetone 99% conversion, 94% enantiomeric excess ?
1.1.1.1 2-fluoro-1-(4-chlorophenyl)ethanone + propan-2-ol in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH Rhodococcus ruber (1R)-2-fluoro-1-(4-chlorophenyl)ethanol + acetone 99% conversion, 98% enantiomeric excess ?
1.1.1.1 2-fluoro-1-(4-fluorophenyl)ethanone + propan-2-ol in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH Rhodococcus ruber (1R)-2-fluoro-1-(4-fluorophenyl)ethanol + acetone 99% conversion, 97% enantiomeric excess ?
1.1.1.1 2-fluoro-1-(4-iodophenyl)ethanone + propan-2-ol in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH Rhodococcus ruber (1R)-2-fluoro-1-(4-iodophenyl)ethanol + acetone 99% conversion, 99% enantiomeric excess ?
1.1.1.1 2-fluoro-1-phenylethanone + propan-2-ol in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH Rhodococcus ruber (1R)-2-fluoro-1-phenylethanol + acetone 99% conversion, 99% enantiomeric excess ?
1.1.1.2 2,2,2-trifluoro-1-phenylethanone + NADPH + H+
-
 Levilactobacillus brevis (1S)-2,2,2-trifluoro-1-phenylethanol + NADP+ 99% conversion, 99% enantiomeric excess ?
1.1.1.2 2,2-difluoro-1-phenylethanone + NADPH + H+
-
 Levilactobacillus brevis (1S)-2,2-difluoro-1-phenylethanol + NADP+ 99% conversion, 99% enantiomeric excess ?
1.1.1.2 2-fluoro-1-(4-chlorophenyl)ethanone + NADPH + H+
-
 Levilactobacillus brevis (1S)-2-fluoro-1-(4-chlorophenyl)ethanol + NADP+ 99% conversion, 99% enantiomeric excess ?
1.1.1.2 2-fluoro-1-(4-fluorophenyl)ethanone + NADPH + H+
-
 Levilactobacillus brevis (1S)-2-fluoro-1-(4-fluorophenyl)ethanol + NADP+ 99% conversion, 99% enantiomeric excess ?
1.1.1.2 2-fluoro-1-(4-iodophenyl)ethanone + NADPH + H+
-
 Levilactobacillus brevis (1S)-2-fluoro-1-(4-iodophenyl)ethanol + NADP+ 99% conversion, 99% enantiomeric excess ?
1.1.1.2 2-fluoro-1-phenylethanone + NADPH + H+
-
 Levilactobacillus brevis (1S)-2-fluoro-1-phenylethanol + NADP+ 99% conversion, 99% enantiomeric excess ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 ADH-A
-
 Rhodococcus ruber
1.1.1.1 SADH
-
 Rhodococcus ruber
1.1.1.2 ADH
-
 Levilactobacillus brevis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 additional information in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH Rhodococcus ruber
1.1.1.2 NADPH
-
 Levilactobacillus brevis