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Literature summary extracted from

  • Itagaki, E.
    Studies on steroid monooxygenase from Cylindrocarpon radicicola ATCC 11011. Oxygenative lactonization of androstenedione to testololactone (1986), J. Biochem., 99, 825-832.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.54 5-Pregnene-3beta,20alpha-diol lactonization of androstenedione Ilyonectria destructans
1.14.13.54 pregnenolone and other C21-20-ketosteroids which are the substrates for oxygenative esterification of the enzyme, strongly inhibit lactonization of androstenedione Ilyonectria destructans
1.14.13.54 progesterone strong, competitive inhibitor of lactonization of 17-ketosteroids Ilyonectria destructans
1.14.13.54 testosterone lactonization of androstenedione Ilyonectria destructans
1.14.13.54 Testosterone acetate lactonization of androstenedione Ilyonectria destructans
1.14.99.12 5-Pregnene-3beta,20alpha-diol
-
Ilyonectria destructans
1.14.99.12 dehydroepiandrosterone
-
Ilyonectria destructans
1.14.99.12 pregnenolone
-
Ilyonectria destructans
1.14.99.12 progesterone
-
Ilyonectria destructans
1.14.99.12 testosterone
-
Ilyonectria destructans
1.14.99.12 Testosterone acetate
-
Ilyonectria destructans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.54 0.0004
-
progesterone
-
Ilyonectria destructans
1.14.13.54 0.002
-
NADPH with androstenedione and O2 as cosubstrates Ilyonectria destructans
1.14.13.54 0.0043
-
NADPH with progesterone and O2 as cosubstrates Ilyonectria destructans
1.14.13.54 0.04
-
androstenedione
-
Ilyonectria destructans
1.14.13.54 0.07
-
O2 with progesterone and NADPH as cosubstrates Ilyonectria destructans
1.14.99.12 0.002
-
NADPH
-
Ilyonectria destructans
1.14.99.12 0.04
-
androst-4-ene-3,17-dione
-
Ilyonectria destructans

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.54 Ilyonectria destructans
-
ATTC 11011
-
1.14.13.54 Ilyonectria destructans ATTC 11011
-
ATTC 11011
-
1.14.99.12 Ilyonectria destructans
-
the steroid monooxygenase also has androstenedione monooxygenase activity
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.54 additional information
-
-
Ilyonectria destructans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.54 17alpha-hydroxypregnenolone + NADPH + O2
-
Ilyonectria destructans ?
-
?
1.14.13.54 17alpha-hydroxypregnenolone + NADPH + O2
-
Ilyonectria destructans ATTC 11011 ?
-
?
1.14.13.54 17alpha-Hydroxyprogesterone + NADPH + O2 the 17-ketosteroid acts first with the enzyme, and then NADPH Ilyonectria destructans androstenedione + acetate + NADP+ + H2O 4-androstene-3,17-dione ?
1.14.13.54 17alpha-Hydroxyprogesterone + NADPH + O2 the 17-ketosteroid acts first with the enzyme, and then NADPH Ilyonectria destructans ATTC 11011 androstenedione + acetate + NADP+ + H2O 4-androstene-3,17-dione ?
1.14.13.54 androstenedione + NADPH + O2
-
Ilyonectria destructans 3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O i.e. testololactone ?
1.14.13.54 androstenedione + NADPH + O2
-
Ilyonectria destructans ATTC 11011 3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O i.e. testololactone ?
1.14.13.54 Pregnenolone + NADPH + O2
-
Ilyonectria destructans ?
-
?
1.14.13.54 Pregnenolone + NADPH + O2
-
Ilyonectria destructans ATTC 11011 ?
-
?
1.14.13.54 progesterone + NADPH + H+ + O2
-
Ilyonectria destructans Testosterone acetate + NADP+ + H2O
-
?
1.14.13.54 progesterone + NADPH + H+ + O2
-
Ilyonectria destructans ATTC 11011 Testosterone acetate + NADP+ + H2O
-
?
1.14.99.12 androst-4-ene-3,17-dione + NADPH + O2
-
Ilyonectria destructans 3-oxo-13,17-secoandrost-4-ene-17,13alpha-lactone + NADP+ + H2O testololactone ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.54 45
-
progesterone monooxygenase activity Ilyonectria destructans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.54 4
-
half-life: 8 days Ilyonectria destructans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.99.12 0.45
-
testololactone formed Ilyonectria destructans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.54 6.5
-
androstenedione monooxygenase reaction Ilyonectria destructans
1.14.13.54 7.8
-
progesterone monooxygenase reaction Ilyonectria destructans
1.14.99.12 6.5
-
-
Ilyonectria destructans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.54 5.5 8 pH 5.5: about 20% of maximal activity, pH 8.0: about 40% of maximal activity, androstenedione monooxygenase activity Ilyonectria destructans
1.14.99.12 5.7 7.8 about 50% of activity maximum at pH 5.7 and 7.8 Ilyonectria destructans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.99.12 NADPH absolute requirement Ilyonectria destructans