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Literature summary extracted from

  • Van der Heiden, E.; Delmarcelle, M.; Simon, P.; Counson, M.; Galleni, M.; Freedberg, D.I.; Thompson, J.; Joris, B.; Battistel, M.D.
    Synthesis and physicochemical characterization of D-tagatose-1-phosphate: the substrate of the tagatose-1-phosphate kinase in the phosphotransferase system-mediated D-tagatose catabolic pathway of catabolic pathway of Bacillus licheniformis (2015), J. Mol. Microbiol. Biotechnol., 25, 106-119.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.234 gene tagK, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.234 D-fructose-6-phosphate
-
Bacillus licheniformis
2.7.1.234 D-tagatose 6-phosphate
-
Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.234 additional information
-
additional information Michaelis-Menten kinetics, the catalytic efficiency of the enzyme for D-tagatose 1-phosphate of 137.5 M/s is 40fold higher than that for fructose 1-phosphate with 3.4 M/s, confirming that the enzyme is a D-tagatose 1-phosphate-specific kinase Bacillus licheniformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.234 Mg2+ required Bacillus licheniformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.234 ATP + D-tagatose 1-phosphate Bacillus licheniformis
-
ADP + D-tagatose 1,6-bisphosphate
-
?
2.7.1.234 ATP + D-tagatose 1-phosphate Klebsiella oxytoca
-
ADP + D-tagatose 1,6-bisphosphate
-
?
2.7.1.234 ATP + D-tagatose 1-phosphate Klebsiella oxytoca M5a1
-
ADP + D-tagatose 1,6-bisphosphate
-
?
2.7.1.234 ATP + D-tagatose 1-phosphate Bacillus licheniformis ATCC 14580
-
ADP + D-tagatose 1,6-bisphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.234 Bacillus licheniformis Q65EY9 annotated as fructose-1-phosphate kinase; gene tagK or BL01904
-
2.7.1.234 Bacillus licheniformis ATCC 14580 Q65EY9 annotated as fructose-1-phosphate kinase; gene tagK or BL01904
-
2.7.1.234 Klebsiella oxytoca Q8VS15 annotated as 1-phosphofructokinase; gene tagK
-
2.7.1.234 Klebsiella oxytoca M5a1 Q8VS15 annotated as 1-phosphofructokinase; gene tagK
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.234 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) b nickel affinity chromatography and desalting gel filtration Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.234 ATP + D-tagatose 1-phosphate
-
Bacillus licheniformis ADP + D-tagatose 1,6-bisphosphate
-
?
2.7.1.234 ATP + D-tagatose 1-phosphate
-
Klebsiella oxytoca ADP + D-tagatose 1,6-bisphosphate
-
?
2.7.1.234 ATP + D-tagatose 1-phosphate the enzyme is absolutely substrate-specific, determination of the phosphate positions by NMR spectroscopy Bacillus licheniformis ADP + D-tagatose 1,6-bisphosphate
-
?
2.7.1.234 ATP + D-tagatose 1-phosphate
-
Klebsiella oxytoca M5a1 ADP + D-tagatose 1,6-bisphosphate
-
?
2.7.1.234 ATP + D-tagatose 1-phosphate
-
Bacillus licheniformis ATCC 14580 ADP + D-tagatose 1,6-bisphosphate
-
?
2.7.1.234 ATP + D-tagatose 1-phosphate the enzyme is absolutely substrate-specific, determination of the phosphate positions by NMR spectroscopy Bacillus licheniformis ATCC 14580 ADP + D-tagatose 1,6-bisphosphate
-
?
2.7.1.234 additional information the bifunctional enzyme shows phospho-tagatokinase and phospho-fructokinase activities, the latter with D-fructose-1-phosphate as substrate Klebsiella oxytoca ?
-
?
2.7.1.234 additional information the bifunctional enzyme shows phospho-tagatokinase and phospho-fructokinase activities, the latter with D-fructose-1-phosphate as substrate. The catalytic efficiency of the enzyme for D-tagatose 1-phosphate is 40fold higher than that for fructose 1-phosphate, confirming that the enzyme is a D-tagatose 1-phosphate-specific kinase Bacillus licheniformis ?
-
?
2.7.1.234 additional information the bifunctional enzyme shows phospho-tagatokinase and phospho-fructokinase activities, the latter with D-fructose-1-phosphate as substrate Klebsiella oxytoca M5a1 ?
-
?
2.7.1.234 additional information the bifunctional enzyme shows phospho-tagatokinase and phospho-fructokinase activities, the latter with D-fructose-1-phosphate as substrate. The catalytic efficiency of the enzyme for D-tagatose 1-phosphate is 40fold higher than that for fructose 1-phosphate, confirming that the enzyme is a D-tagatose 1-phosphate-specific kinase Bacillus licheniformis ATCC 14580 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.234 phospho-tagatokinase
-
Bacillus licheniformis
2.7.1.234 tagatose-1P kinase
-
Bacillus licheniformis
2.7.1.234 TagK
-
Bacillus licheniformis
2.7.1.234 TagK
-
Klebsiella oxytoca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.234 25
-
assay at Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.234 8
-
assay at Bacillus licheniformis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.234 ATP
-
Bacillus licheniformis
2.7.1.234 ATP
-
Klebsiella oxytoca

General Information

EC Number General Information Comment Organism
2.7.1.234 additional information structure homology modeling of tagatose-1P kinase using the structure of fructose 1-phosphate kinase from Bacillus halodurans, PDB ID 2ABQ Bacillus licheniformis