EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.234 | gene tagK, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Bacillus licheniformis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.234 | D-fructose-6-phosphate | - |
Bacillus licheniformis | |
2.7.1.234 | D-tagatose 6-phosphate | - |
Bacillus licheniformis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.234 | additional information | - |
additional information | Michaelis-Menten kinetics, the catalytic efficiency of the enzyme for D-tagatose 1-phosphate of 137.5 M/s is 40fold higher than that for fructose 1-phosphate with 3.4 M/s, confirming that the enzyme is a D-tagatose 1-phosphate-specific kinase | Bacillus licheniformis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.234 | Mg2+ | required | Bacillus licheniformis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.234 | ATP + D-tagatose 1-phosphate | Bacillus licheniformis | - |
ADP + D-tagatose 1,6-bisphosphate | - |
? | |
2.7.1.234 | ATP + D-tagatose 1-phosphate | Klebsiella oxytoca | - |
ADP + D-tagatose 1,6-bisphosphate | - |
? | |
2.7.1.234 | ATP + D-tagatose 1-phosphate | Klebsiella oxytoca M5a1 | - |
ADP + D-tagatose 1,6-bisphosphate | - |
? | |
2.7.1.234 | ATP + D-tagatose 1-phosphate | Bacillus licheniformis ATCC 14580 | - |
ADP + D-tagatose 1,6-bisphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.234 | Bacillus licheniformis | Q65EY9 | annotated as fructose-1-phosphate kinase; gene tagK or BL01904 | - |
2.7.1.234 | Bacillus licheniformis ATCC 14580 | Q65EY9 | annotated as fructose-1-phosphate kinase; gene tagK or BL01904 | - |
2.7.1.234 | Klebsiella oxytoca | Q8VS15 | annotated as 1-phosphofructokinase; gene tagK | - |
2.7.1.234 | Klebsiella oxytoca M5a1 | Q8VS15 | annotated as 1-phosphofructokinase; gene tagK | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.234 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) b nickel affinity chromatography and desalting gel filtration | Bacillus licheniformis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.234 | ATP + D-tagatose 1-phosphate | - |
Bacillus licheniformis | ADP + D-tagatose 1,6-bisphosphate | - |
? | |
2.7.1.234 | ATP + D-tagatose 1-phosphate | - |
Klebsiella oxytoca | ADP + D-tagatose 1,6-bisphosphate | - |
? | |
2.7.1.234 | ATP + D-tagatose 1-phosphate | the enzyme is absolutely substrate-specific, determination of the phosphate positions by NMR spectroscopy | Bacillus licheniformis | ADP + D-tagatose 1,6-bisphosphate | - |
? | |
2.7.1.234 | ATP + D-tagatose 1-phosphate | - |
Klebsiella oxytoca M5a1 | ADP + D-tagatose 1,6-bisphosphate | - |
? | |
2.7.1.234 | ATP + D-tagatose 1-phosphate | - |
Bacillus licheniformis ATCC 14580 | ADP + D-tagatose 1,6-bisphosphate | - |
? | |
2.7.1.234 | ATP + D-tagatose 1-phosphate | the enzyme is absolutely substrate-specific, determination of the phosphate positions by NMR spectroscopy | Bacillus licheniformis ATCC 14580 | ADP + D-tagatose 1,6-bisphosphate | - |
? | |
2.7.1.234 | additional information | the bifunctional enzyme shows phospho-tagatokinase and phospho-fructokinase activities, the latter with D-fructose-1-phosphate as substrate | Klebsiella oxytoca | ? | - |
? | |
2.7.1.234 | additional information | the bifunctional enzyme shows phospho-tagatokinase and phospho-fructokinase activities, the latter with D-fructose-1-phosphate as substrate. The catalytic efficiency of the enzyme for D-tagatose 1-phosphate is 40fold higher than that for fructose 1-phosphate, confirming that the enzyme is a D-tagatose 1-phosphate-specific kinase | Bacillus licheniformis | ? | - |
? | |
2.7.1.234 | additional information | the bifunctional enzyme shows phospho-tagatokinase and phospho-fructokinase activities, the latter with D-fructose-1-phosphate as substrate | Klebsiella oxytoca M5a1 | ? | - |
? | |
2.7.1.234 | additional information | the bifunctional enzyme shows phospho-tagatokinase and phospho-fructokinase activities, the latter with D-fructose-1-phosphate as substrate. The catalytic efficiency of the enzyme for D-tagatose 1-phosphate is 40fold higher than that for fructose 1-phosphate, confirming that the enzyme is a D-tagatose 1-phosphate-specific kinase | Bacillus licheniformis ATCC 14580 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.234 | phospho-tagatokinase | - |
Bacillus licheniformis |
2.7.1.234 | tagatose-1P kinase | - |
Bacillus licheniformis |
2.7.1.234 | TagK | - |
Bacillus licheniformis |
2.7.1.234 | TagK | - |
Klebsiella oxytoca |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.234 | 25 | - |
assay at | Bacillus licheniformis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.234 | 8 | - |
assay at | Bacillus licheniformis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.234 | ATP | - |
Bacillus licheniformis | |
2.7.1.234 | ATP | - |
Klebsiella oxytoca |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.1.234 | additional information | structure homology modeling of tagatose-1P kinase using the structure of fructose 1-phosphate kinase from Bacillus halodurans, PDB ID 2ABQ | Bacillus licheniformis |