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Literature summary extracted from

  • Verma, A.; Goyal, A.
    A novel member of family 30 glycoside hydrolase subfamily 8 glucuronoxylan endo-beta-1,4-xylanase (CtXynGH30) from Clostridium thermocellum orchestrates catalysis on arabinose decorated xylans (2016), J. Mol. Catal. B, 129, 6-14.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8
-
 Acetivibrio thermocellus
3.2.1.136
-
 Acetivibrio thermocellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 60000
-
-
 Acetivibrio thermocellus
3.2.1.8 61200
-
-
 Acetivibrio thermocellus
3.2.1.136 60000
-
-
 Acetivibrio thermocellus
3.2.1.136 61200
-
-
 Acetivibrio thermocellus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Acetivibrio thermocellus
-
-
-
3.2.1.136 Acetivibrio thermocellus A3DJS9
-
-
3.2.1.136 Acetivibrio thermocellus DSM 1237 A3DJS9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8
-
 Acetivibrio thermocellus
3.2.1.136
-
 Acetivibrio thermocellus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 30
-
 substrate 4-O-methyl-beta-D-glucuronoxylan, pH 6.0, 70°C Acetivibrio thermocellus
3.2.1.8 31
-
 substrate birchwood xylan, pH 6.0, 70°C Acetivibrio thermocellus
3.2.1.8 34
-
 substrate beechwood glucuronoxylan, pH 6.0, 70°C Acetivibrio thermocellus
3.2.1.136 9.5
-
 substrate wheat arabinoxylan , pH 6.0, 70°C Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 4-O-methyl-beta-D-glucuronoxylan + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.2.1.8 beechwood glucuronoxylan + H2O
-
 Acetivibrio thermocellus ? products are acidic xylo-oligosaccharides having methyl glucuronic acid moiety penultimate to the reducing end of xylan ?
3.2.1.8 birchwood xylan + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.2.1.8 additional information enzyme also acts on arabinoxylans, reaction of EC 3.2.1.55 Acetivibrio thermocellus ?
-
 ?
3.2.1.136 additional information enzyme also acts on xylans, reaction of EC 3.2.1.8 Acetivibrio thermocellus ?
-
 ?
3.2.1.136 additional information enzyme also acts on xylans, reaction of EC 3.2.1.8 Acetivibrio thermocellus DSM 1237 ?
-
 ?
3.2.1.136 wheat arabinoxylan + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.2.1.136 wheat arabinoxylan + H2O
-
 Acetivibrio thermocellus DSM 1237 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 60000, SDS-PAGE, x * 61200, calculated Acetivibrio thermocellus
3.2.1.136 ? x * 60000, SDS-PAGE, x * 61200, calculated Acetivibrio thermocellus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 XynGH30
-
 Acetivibrio thermocellus
3.2.1.136 XynGH30
-
 Acetivibrio thermocellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 70
-
-
 Acetivibrio thermocellus
3.2.1.136 70
-
-
 Acetivibrio thermocellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6
-
-
 Acetivibrio thermocellus
3.2.1.136 6
-
-
 Acetivibrio thermocellus