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Literature summary extracted from

  • Virgen-OrtÃiz, J.; Ibarra-Junquera, V.; Escalante-Minakata, P.; Ornelas-Paz, J.; Osuna-Castro, J.; Gonzalez-Potes, A.
    Kinetics and thermodynamic of the purified dextranase from Chaetomium erraticum (2015), J. Mol. Catal. B, 122, 80-86.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.11 Cu2+ 1 mM, 32% resiudal activity Chaetomium erraticum
3.2.1.11 Fe3+ 1 mM, 84% resiudal activity Chaetomium erraticum
3.2.1.11 Hg2+ 1 mM, no resiudal activity Chaetomium erraticum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.11 0.043
-
 Dextran pH 5.2, 30°C Chaetomium erraticum
3.2.1.11 0.075
-
 Dextran pH 5.2, 40°C Chaetomium erraticum
3.2.1.11 0.147
-
 Dextran pH 5.2, 50°C Chaetomium erraticum
3.2.1.11 0.26
-
 Dextran pH 5.2, 60°C Chaetomium erraticum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.11 Ca2+ 1 mM, 117% of initial activity Chaetomium erraticum
3.2.1.11 Co2+ 1 mM, 124% of initial activity Chaetomium erraticum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.11 59000
-
 2 * 59000, SDS-PAGE Chaetomium erraticum
3.2.1.11 120000
-
 PAGE Chaetomium erraticum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.11 Chaetomium erraticum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.11
-
 Chaetomium erraticum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.11 1106
-
 pH 5.2, 60°C Chaetomium erraticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.11 dextran + H2O 100 kDa dextran Chaetomium erraticum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.11 dimer 2 * 59000, SDS-PAGE Chaetomium erraticum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.11 60
-
-
 Chaetomium erraticum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.11 55 65 more than 85% of maximum activity Chaetomium erraticum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.11 723
-
 Dextran pH 5.2, 30°C Chaetomium erraticum
3.2.1.11 1362
-
 Dextran pH 5.2, 40°C Chaetomium erraticum
3.2.1.11 2865
-
 Dextran pH 5.2, 50°C Chaetomium erraticum
3.2.1.11 4564
-
 Dextran pH 5.2, 60°C Chaetomium erraticum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.11 5.2
-
-
 Chaetomium erraticum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.11 4 6 more than 85% of maximum activity Chaetomium erraticum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.11 16800
-
 Dextran pH 5.2, 30°C Chaetomium erraticum
3.2.1.11 17600
-
 Dextran pH 5.2, 60°C Chaetomium erraticum
3.2.1.11 18300
-
 Dextran pH 5.2, 40°C Chaetomium erraticum
3.2.1.11 19500
-
 Dextran pH 5.2, 50°C Chaetomium erraticum