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Literature summary extracted from

  • Rakitin, A.L.; Ermakova, A.Y.; Ravin, N.V.
    Novel endoxylanases of the moderately thermophilic polysaccharide-degrading bacterium Melioribacter roseus (2015), J. Microbiol. Biotechnol., 25, 1476-1484.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Cd2+ 2 mM, 10% residual activity; 2 mM, 21% residual activity; 2 mM, 25% residual activity Melioribacter roseus
3.2.1.8 Cu2+ 2 mM, 77% residual activity; 2 mM, no residual activity; 2 mM, no residual activity Melioribacter roseus
3.2.1.8 Hg2+ 2 mM, 23% residual activity; 2 mM, 73% residual activity; 2 mM, no residual activity Melioribacter roseus
3.2.1.8 Ni2+ 2 mM, 28% residual activity; 2 mM, 63% residual activity; 2 mM, 78% residual activity Melioribacter roseus
3.2.1.8 Pb2+ 2 mM, 18% residual activity; 2 mM, 46% residual activity; 2 mM, no residual activity Melioribacter roseus
3.2.1.8 Zn2+ 2 mM, 39% residual activity; 2 mM, 57% residual activity; 2 mM, 5% residual activity Melioribacter roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 birchwood xylan Km value 1.05 mg/ml, pH 6.5, 65°C Melioribacter roseus
3.2.1.8 additional information
-
 birchwood xylan Km value 10.5 mg/ml, pH 6.5, 80°C Melioribacter roseus
3.2.1.8 additional information
-
 birchwood xylan Km value 3.0 mg/ml, pH 6.5, 65°C Melioribacter roseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 42000
-
 x * 42000, SDS-PAGE Melioribacter roseus
3.2.1.8 68000
-
 x * 68000, SDS-PAGE Melioribacter roseus
3.2.1.8 86000
-
 x * 86000, SDS-PAGE Melioribacter roseus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Melioribacter roseus I6Z823 cf. EC 3.2.1.136
-
3.2.1.8 Melioribacter roseus I6Z9A7
-
-
3.2.1.8 Melioribacter roseus I7A603
-
-
3.2.1.8 Melioribacter roseus JCM 17771 I6Z823 cf. EC 3.2.1.136
-
3.2.1.8 Melioribacter roseus JCM 17771 I6Z9A7
-
-
3.2.1.8 Melioribacter roseus JCM 17771 I7A603
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 41
-
 substrate birchwood xylan, pH 6.5, 80°C Melioribacter roseus
3.2.1.8 41.8
-
 substrate beechwood xylan, pH 6.5, 65°C Melioribacter roseus
3.2.1.8 55
-
 substrate beechwood xylan, pH 6.5, 80°C Melioribacter roseus
3.2.1.8 75
-
 substrate birchwood xylan, pH 6.5, 65°C Melioribacter roseus
3.2.1.8 1352
-
 substrate beechwood xylan, pH 6.5, 65°C Melioribacter roseus
3.2.1.8 1920
-
 substrate birchwood xylan, pH 6.5, 65°C Melioribacter roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beechwood xylan + H2O
-
 Melioribacter roseus xylohexaose + ? and larger products ?
3.2.1.8 beechwood xylan + H2O
-
 Melioribacter roseus JCM 17771 xylohexaose + ? and larger products ?
3.2.1.8 beechwood xylan + H2O
-
 Melioribacter roseus xylose + xylobiose + ? main products ?
3.2.1.8 beechwood xylan + H2O
-
 Melioribacter roseus JCM 17771 xylose + xylobiose + ? main products ?
3.2.1.8 birchwood xylan + H2O
-
 Melioribacter roseus xylohexaose + ? and larger products ?
3.2.1.8 birchwood xylan + H2O
-
 Melioribacter roseus JCM 17771 xylohexaose + ? and larger products ?
3.2.1.8 birchwood xylan + H2O
-
 Melioribacter roseus xylose + xylobiose + ? main products ?
3.2.1.8 birchwood xylan + H2O
-
 Melioribacter roseus JCM 17771 xylose + xylobiose + ? main products ?
3.2.1.8 additional information no substrate: cellulose Melioribacter roseus ?
-
 ?
3.2.1.8 additional information no substrate: cellulose Melioribacter roseus JCM 17771 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 42000, SDS-PAGE Melioribacter roseus
3.2.1.8 ? x * 86000, SDS-PAGE Melioribacter roseus
3.2.1.8 ? x * 68000, SDS-PAGE Melioribacter roseus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 MROS_2090
-
 Melioribacter roseus
3.2.1.8 MROS_2091
-
 Melioribacter roseus
3.2.1.8 MROS_2495
-
 Melioribacter roseus
3.2.1.8 Xyl2090
-
 Melioribacter roseus
3.2.1.8 Xyl2091
-
 Melioribacter roseus
3.2.1.8 Xyl2495
-
 Melioribacter roseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 65
-
-
 Melioribacter roseus
3.2.1.8 80
-
-
 Melioribacter roseus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 80
-
 half-life 1 h Melioribacter roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6.5
-
-
 Melioribacter roseus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 9
-
 50% of maximum activity Melioribacter roseus