Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ma, F.; Wang, T.; Ma, X.; Wang, P.
    Identification and characterization of protein encoded by orf382 as L-threonine dehydrogenase (2014), J. Microbiol. Biotechnol., 24, 748-755.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.103 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.103 0.00802
-
 n-butanol at pH 8.8 and 37°C Escherichia coli
1.1.1.103 0.01129
-
 L-threonine at pH 8.8 and 37°C Escherichia coli
1.1.1.103 0.2225
-
 ethanol at pH 8.8 and 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.103 Iron the protein contains 0.424 mol of Fe per mol Escherichia coli
1.1.1.103 Zn2+ the protein contains 0.914 mol of Zn2+ per mol Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.103 40000
-
 x * 40000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.103 Escherichia coli P07913
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.103 Ni-NTA agarose column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.103 citric acid + NAD+ low activity Escherichia coli ?
-
 ?
1.1.1.103 D-glucose + NAD+
-
 Escherichia coli ?
-
 ?
1.1.1.103 D-threonine + NAD+ lowest activity Escherichia coli (2R)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
1.1.1.103 ethanol + NAD+ high activity Escherichia coli acetaldehyde + NADH + H+
-
 ?
1.1.1.103 glycerol + NAD+
-
 Escherichia coli ?
-
 ?
1.1.1.103 isopropanol + NAD+
-
 Escherichia coli isopropylaldehyde + NADH + H+
-
 ?
1.1.1.103 L-serine + NAD+
-
 Escherichia coli 3-oxo-L-alanine + NADH + H+
-
 ?
1.1.1.103 L-threonine + NAD+ high activity Escherichia coli (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
1.1.1.103 lactic acid + NAD+ low activity Escherichia coli ?
-
 ?
1.1.1.103 methanol + NAD+ low activity Escherichia coli formaldehyde + NADH + H+
-
 ?
1.1.1.103 n-butanol + NAD+ highest activity Escherichia coli butanal + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.103 ? x * 40000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.1.1.103 L-threonine dehydrogenase
-
 Escherichia coli
1.1.1.103 orf382
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.103 39
-
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.103 9.8
-
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.103 NAD+ the enzyme exhibits an apparent preference for NAD+ over NADP+ Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.103 Escherichia coli isoelectric focusing
-
 5.4