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Literature summary extracted from

  • Wijesinghe, D.S.; Brentnall, M.; Mietla, J.A.; Hoeferlin, L.A.; Diegelmann, R.F.; Boise, L.H.; Chalfant, C.E.
    Ceramide kinase is required for a normal eicosanoid response and the subsequent orderly migration of fibroblasts (2014), J. Lipid Res., 55, 1298-1309.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.138 additional information generation of CERK-/- knockout mice Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.138 Mg2+ required Homo sapiens
2.7.1.138 Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.138 ATP + ceramide Homo sapiens
-
 ADP + ceramide 1-phosphate
-
 ?
2.7.1.138 ATP + ceramide Mus musculus
-
 ADP + ceramide 1-phosphate
-
 ?
2.7.1.138 ATP + ceramide Mus musculus BALB/c
-
 ADP + ceramide 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.138 Homo sapiens Q8TCT0 gene CERK
-
2.7.1.138 Mus musculus Q8K4Q7 gene CERK
-
2.7.1.138 Mus musculus BALB/c Q8K4Q7 gene CERK
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.138 fibroblast
-
 Homo sapiens
-
2.7.1.138 fibroblast
-
 Mus musculus
-
2.7.1.138 MEF cell primary mouse embryonic fibroblasts Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.138 ATP + ceramide
-
 Homo sapiens ADP + ceramide 1-phosphate
-
 ?
2.7.1.138 ATP + ceramide
-
 Mus musculus ADP + ceramide 1-phosphate
-
 ?
2.7.1.138 ATP + ceramide
-
 Mus musculus BALB/c ADP + ceramide 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.138 CERK
-
 Homo sapiens
2.7.1.138 CERK
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.138 ATP
-
 Homo sapiens
2.7.1.138 ATP
-
 Mus musculus

General Information

EC Number General Information Comment Organism
2.7.1.138 malfunction genetic ablation of CERK results in decreased levels of ceramide 1-phosphate in fibroblasts and inhibits the ability of fibroblasts to release arachidonic acid in response to mechanical trauma. Simulation of mechanical trauma of a wound by scratching a monolayer of fibroblasts from CERK+/+ mice demonstrates steadily increasing levels of arachidonic acid in a time-dependent manner in stark contrast to CERK-/- fibroblasts, reflected in scratch-induced eicosanoid levels. Loss of proper eicosanoid response translated into an abnormal migration pattern for the fibroblasts isolated from CERK-/-. Neither the wild-type nor the CERK-null fibroblasts demonstrated additional changes to the endogenous ceramide 1-phosphate content upon induction of mechanical trauma. The migratory response to wounding of CERK-/- fibroblasts can be rescued via the addition of exogenous eicosanoids Mus musculus
2.7.1.138 physiological function CERK is required for eicosanoid biosynthesis in response to mechanical insult. Proper migration of fibroblasts is one of the necessary steps of wound healing, requirement for the CERK-derived ceramide 1-phosphate in the proper healing response of wounds Mus musculus
2.7.1.138 physiological function CERK is required for eicosanoid biosynthesis in response to mechanical insult. Simulation of mechanical trauma of a wound demonstrates steadily increasing levels of arachidonic acid in a time-dependent manner reflected in scratch-induced eicosanoid levels. Proper migration of fi broblasts is one of the necessary steps of wound healing, requirement for the CERK-derived ceramide 1-phosphate in the proper healing response of wounds Homo sapiens