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Literature summary extracted from

  • Kim, B.; Ahn, J.
    Characterization of uridine diphosphate-sugar pyrophosphorylase from Populus deltoids (2013), J. Korean Soc. Appl. Biol. Chem., 56, 525-531.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.64 expression in Escherichia coli Populus deltoides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.64 1.49
-
 D-glucose 1-phosphate pH 8.0, 37°C Populus deltoides
2.7.7.64 1.98
-
 UTP pH 8.0, 37°C Populus deltoides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.64 77500
-
-
 Populus deltoides

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.64 Populus deltoides
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.64 D-galactose 1-phosphate + UTP 30% of the activity with D-glucose 1-phosphate Populus deltoides UDP-D-galactose + diphosphate
-
 ?
2.7.7.64 D-glucose 1-phosphate + UTP
-
 Populus deltoides UDP-D-glucose + diphosphate
-
 ?
2.7.7.64 additional information no substrate: D-mannose 1-phosphate Populus deltoides ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.64 ? x * 77500, SDS-PAGE, recombinant protein with GHST-tag Populus deltoides

Synonyms

EC Number Synonyms Comment Organism
2.7.7.64 USPase
-
 Populus deltoides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.64 0.003
-
 UTP pH 8.0, 37°C Populus deltoides
2.7.7.64 0.008
-
 D-glucose 1-phosphate pH 8.0, 37°C Populus deltoides

General Information

EC Number General Information Comment Organism
2.7.7.64 physiological function enzyme is able to complement an Escherichia coli GalU mutant Populus deltoides

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.64 0.0015
-
 UTP pH 8.0, 37°C Populus deltoides
2.7.7.64 0.0054
-
 D-glucose 1-phosphate pH 8.0, 37°C Populus deltoides