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Literature summary extracted from

  • Van Ekert, E.; Heylen, K.; Rouge, P.; Powell, C.A.; Shatters, R.G.; Smagghe, G.; Borovsky, D.
    Aedes aegypti juvenile hormone acid methyl transferase, the ultimate enzyme in the biosynthetic pathway of juvenile hormone III, exhibits substrate control (2014), J. Insect Physiol., 64, 62-73.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.325 expressed in Escherichia coli One Shot BL21 (DE3) cells Aedes aegypti

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.325 additional information not influenced by EDTA Aedes aegypti
2.1.1.325 Zn2+ 16fold inhibition at 10 mM Aedes aegypti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.325 0.000008
-
 (trans/cis/cis)-juvenile hormone I acid at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.00016
-
 juvenile hormone III acid bisepoxide at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.00028
-
 juvenile hormone I acid bisepoxide at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.00223
-
 (2E,6E)-farnesoate at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.00387
-
 (cis/trans/cis)-juvenile hormone I acid at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.01027
-
 (cis/trans/trans)-juvenile hormone I acid at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.01468
-
 juvenile hormone III acid at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.01997
-
 homofarnesoate at pH 7.9 and 25°C Aedes aegypti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.325 additional information metal ions are not required for enzyme activity Aedes aegypti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.325 32000
-
 x * 32000, SDS-PAGE Aedes aegypti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.325 S-adenosyl-L-methionine + (2E,6E)-farnesoate Aedes aegypti
-
 S-adenosyl-L-homocysteine + methyl (2E,6E)-farnesoate
-
 ?
2.1.1.325 S-adenosyl-L-methionine + juvenile hormone III acid Aedes aegypti
-
 S-adenosyl-L-homocysteine + juvenile hormone III
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.325 Aedes aegypti Q206L4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.325 Ni-NTA column chromatography, and gel filtration Aedes aegypti

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.325 corpora allata
-
 Aedes aegypti
-
2.1.1.325 ovary
-
 Aedes aegypti
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.325 S-adenosyl-L-methionine + (2E,6E)-farnesoate
-
 Aedes aegypti S-adenosyl-L-homocysteine + methyl (2E,6E)-farnesoate
-
 ?
2.1.1.325 S-adenosyl-L-methionine + (cis/trans/cis)-juvenile hormone I acid
-
 Aedes aegypti ?
-
 ?
2.1.1.325 S-adenosyl-L-methionine + (cis/trans/trans)-juvenile hormone I acid
-
 Aedes aegypti ?
-
 ?
2.1.1.325 S-adenosyl-L-methionine + (trans/cis/cis)-juvenile hormone I acid
-
 Aedes aegypti ?
-
 ?
2.1.1.325 S-adenosyl-L-methionine + homofarnesoate
-
 Aedes aegypti S-adenosyl-L-homocysteine + methylhomofarnesoate
-
 ?
2.1.1.325 S-adenosyl-L-methionine + juvenile hormone I acid bisepoxide
-
 Aedes aegypti ?
-
 ?
2.1.1.325 S-adenosyl-L-methionine + juvenile hormone III acid
-
 Aedes aegypti S-adenosyl-L-homocysteine + juvenile hormone III
-
 ?
2.1.1.325 S-adenosyl-L-methionine + juvenile hormone III acid bisepoxide
-
 Aedes aegypti ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.325 ? x * 32000, SDS-PAGE Aedes aegypti

Synonyms

EC Number Synonyms Comment Organism
2.1.1.325 JHAMT
-
 Aedes aegypti
2.1.1.325 juvenile hormone acid methyl transferase
-
 Aedes aegypti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.325 0.000006
-
 (trans/cis/cis)-juvenile hormone I acid at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.00001
-
 juvenile hormone III acid bisepoxide at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.000011
-
 juvenile hormone I acid bisepoxide at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.00016
-
 homofarnesoate at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.000217
-
 (2E,6E)-farnesoate at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.000245
-
 (cis/trans/cis)-juvenile hormone I acid at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.000875
-
 (cis/trans/trans)-juvenile hormone I acid at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.001148
-
 juvenile hormone III acid at pH 7.9 and 25°C Aedes aegypti

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.325 0.00803
-
 homofarnesoate at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.03929
-
 juvenile hormone I acid bisepoxide at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.06146
-
 juvenile hormone III acid bisepoxide at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.06335
-
 (cis/trans/cis)-juvenile hormone I acid at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.0782
-
 juvenile hormone III acid at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.08523
-
 (cis/trans/trans)-juvenile hormone I acid at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.09716
-
 (2E,6E)-farnesoate at pH 7.9 and 25°C Aedes aegypti
2.1.1.325 0.7708
-
 (trans/cis/cis)-juvenile hormone I acid at pH 7.9 and 25°C Aedes aegypti