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Literature summary extracted from

  • Kamiya, H.; Ito, M.; Nishi, K.; Harashima, H.
    In vivo selection of active deoxyribonucleoside kinase by a mutagenic nucleoside analog (2016), J. Biotechnol., 228, 52-57.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.145 gene dnk, isolated from a phage library, DNA and amino acid sequence determination and analysis, recombinant expression of randomly mutated genes for deoxyribonucleoside kinase in Escherichia coli strain BL21, recombinant expression of catalytically active His10-tagged mutant 4 enzyme in Spodoptera frugiperda SF9 cells Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.145 EDTA
-
Drosophila melanogaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.145 Mg2+ required Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.145 ATP + 2'-deoxyribonucleoside Drosophila melanogaster
-
ADP + 2'-deoxyribonucleoside 5'-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.145 Drosophila melanogaster Q9XZT6 gene dnk
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.145 recombinant His10-tagged enzyme from Spodoptera frugiperda SF9 cells by nickel affinity chromatography and gel filtration Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.145 ATP + 2'-deoxycytidine
-
Drosophila melanogaster ADP + 2'-deoxycytidine 5'-phosphate
-
?
2.7.1.145 ATP + 2'-deoxyribonucleoside
-
Drosophila melanogaster ADP + 2'-deoxyribonucleoside 5'-phosphate
-
?
2.7.1.145 ATP + 2-hydroxy-2'-deoxyadenosine preferred substrate for enzyme mutant 4 Drosophila melanogaster ADP + 2-hydroxy-2'-deoxyadenosine 5'-monophosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.145 Dm-dNK
-
Drosophila melanogaster
2.7.1.145 multisubstrate deoxyribonucleoside kinase
-
Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.145 37
-
assay at Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.145 8
-
assay at Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.145 ATP
-
Drosophila melanogaster

General Information

EC Number General Information Comment Organism
2.7.1.145 additional information enzyme identification using a mutagenic nucleoside analogue 2-hydroxy-2'-deoxyadenosine 5'-monophosphate, overview. Method development and evaluation for in vivo evolution selection method for active deoxyribonucleoside kinase proteins, including selection of antibiotic-resistant colonies resulting frommutations by phosphorylated 2-hydroxy-deoxyadenosine and the subsequent isolation of the plasmid DNAs. Fifteen Dm-dNK mutants selected after the seventh and eighth evolutioncycles are actually active in vivo, one shows activity similar to the wild-typ enzyme Drosophila melanogaster