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Literature summary extracted from

  • Magomedova, Z.; Grecu, A.; Sensen, C.W.; Schwab, H.; Heidinger, P.
    Characterization of two novel alcohol short-chain dehydrogenases/reductases from Ralstonia eutropha H16 capable of stereoselective conversion of bulky substrates (2016), J. Biotechnol., 221, 78-90.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.91 expressed in Escherichia coli Top10 cells Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.91 7.1
-
 (S)-2-octanol isoform SDR B3, at pH 10.0 and 40°C Cupriavidus necator
1.1.1.91 7.7
-
 (S)-2-octanol isoform SDR A5, at pH 10.0 and 40°C Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.91 Cupriavidus necator
-
-
-
1.1.1.91 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.91 Ni-Sepharose column chromatography Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.91 (R)-2-octanol + NADPH + H+
-
 Cupriavidus necator ?
-
 ?
1.1.1.91 (R)-2-octanol + NADPH + H+
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?
1.1.1.91 (S)-2-octanol + NADPH + H+
-
 Cupriavidus necator ?
-
 ?
1.1.1.91 (S)-2-octanol + NADPH + H+
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?
1.1.1.91 2-methyl-propanol + NADPH + H+
-
 Cupriavidus necator ?
-
 ?
1.1.1.91 2-methyl-propanol + NADPH + H+
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?
1.1.1.91 3-methyl-butanol + NADPH + H+
-
 Cupriavidus necator ?
-
 ?
1.1.1.91 benzil + NADPH + H+
-
 Cupriavidus necator benzoin + NADP+
-
 ?
1.1.1.91 butanol + NADPH + H+
-
 Cupriavidus necator ?
-
 ?
1.1.1.91 additional information no activity is detected for isoform SDR A5 with isobutyraldehyde, isovaleraldehyde, 2-heptanone, 2-phenylacetaldehyde, 1-phenyl-1-butanone, 1-phenyl-1-pentanone, 1-phenyl-1-hexanone, 1-phenyl-2-propanone, 2-hydroxyacetophenone, 2'-hydroxyacetophenone, 1-phenylpropane-1,2-dione, benzophenone, (R)-(-)-benzoin, propanol, butanol, rac-2-propanol, 2-methylpropanol, 3-methylbutanol, rac-3-methyl-2-butanol, (S)-4-methyl-2-pentanol, (R)-4-methyl-2-pentanol, (R)-2-hexanol, (R)-2-octanol, rac-1-amino-2-propanol, rac-1-bromo-2-propanol, butan-2,3-diol, rac-decan-1,2-diol, 3-hydroxybutanoic acid, 2-hexyn-1-ol, (R)-1-phenylethanol, 2-phenylethanol, rac-benzoin, meso-hydrobenzoin, (S,S)-hydrobenzoin, and (R,R)-hydrobenzoin. No activity is detected for isoform SDR B3 with isobutyraldehyde, 2-hexanone, 2-heptanone, 2-octanone, ethyl acetoacetate, acetophenone, 1-phenyl-1-butanone, 1-phenyl-1-pentanone, 1-phenyl-1-hexanone, 1-phenyl-2-propanone, 2-hydroxyacetophenone, 2'-hydroxyacetophenone, 4-(4-hydroxyphenyl)-2-butanone, 1-phenylpropane-1,2-dione, benzophenone, rac-benzoin, (R)-benzoin, propanol, butanol, rac-2-propanol, 2-methylpropanol, 3-methylbutanol, (R)-4-methyl-2-pentanol, (R)-2-hexanol, rac-1-amino-2-propanol, rac-1-bromo-2-propanol, butan-2,3-diol, 3-hydroxybutanoic acid, 2-hexyn-1-ol, (R)-1-phenylethanol, 2-phenylethanol, rac-benzoin, meso-hydrobenzoin, (S,S)-hydrobenzoin, and (R,R)-hydrobenzoin Cupriavidus necator ?
-
 ?
1.1.1.91 additional information the enzyme accepts both NAD+ and NADP+. However, purified enzyme shows strong preference for NADP+ Cupriavidus necator ?
-
 ?
1.1.1.91 additional information no activity is detected for isoform SDR A5 with isobutyraldehyde, isovaleraldehyde, 2-heptanone, 2-phenylacetaldehyde, 1-phenyl-1-butanone, 1-phenyl-1-pentanone, 1-phenyl-1-hexanone, 1-phenyl-2-propanone, 2-hydroxyacetophenone, 2'-hydroxyacetophenone, 1-phenylpropane-1,2-dione, benzophenone, (R)-(-)-benzoin, propanol, butanol, rac-2-propanol, 2-methylpropanol, 3-methylbutanol, rac-3-methyl-2-butanol, (S)-4-methyl-2-pentanol, (R)-4-methyl-2-pentanol, (R)-2-hexanol, (R)-2-octanol, rac-1-amino-2-propanol, rac-1-bromo-2-propanol, butan-2,3-diol, rac-decan-1,2-diol, 3-hydroxybutanoic acid, 2-hexyn-1-ol, (R)-1-phenylethanol, 2-phenylethanol, rac-benzoin, meso-hydrobenzoin, (S,S)-hydrobenzoin, and (R,R)-hydrobenzoin. No activity is detected for isoform SDR B3 with isobutyraldehyde, 2-hexanone, 2-heptanone, 2-octanone, ethyl acetoacetate, acetophenone, 1-phenyl-1-butanone, 1-phenyl-1-pentanone, 1-phenyl-1-hexanone, 1-phenyl-2-propanone, 2-hydroxyacetophenone, 2'-hydroxyacetophenone, 4-(4-hydroxyphenyl)-2-butanone, 1-phenylpropane-1,2-dione, benzophenone, rac-benzoin, (R)-benzoin, propanol, butanol, rac-2-propanol, 2-methylpropanol, 3-methylbutanol, (R)-4-methyl-2-pentanol, (R)-2-hexanol, rac-1-amino-2-propanol, rac-1-bromo-2-propanol, butan-2,3-diol, 3-hydroxybutanoic acid, 2-hexyn-1-ol, (R)-1-phenylethanol, 2-phenylethanol, rac-benzoin, meso-hydrobenzoin, (S,S)-hydrobenzoin, and (R,R)-hydrobenzoin Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?
1.1.1.91 additional information the enzyme accepts both NAD+ and NADP+. However, purified enzyme shows strong preference for NADP+ Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?
1.1.1.91 rac-4-methyl-2-pentanol + NADPH + H+
-
 Cupriavidus necator ?
-
 ?
1.1.1.91 rac-butan-2,3-diol + NADPH + H+
-
 Cupriavidus necator ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.91 SDR A5
-
 Cupriavidus necator
1.1.1.91 SDR B3
-
 Cupriavidus necator
1.1.1.91 short-chain dehydrogenase/reductase
-
 Cupriavidus necator

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.91 40
-
 isoform SDR A5 Cupriavidus necator
1.1.1.91 50
-
 isoform SDR B3 Cupriavidus necator

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.91 4.3
-
 (S)-2-octanol isoform SDR B3, at pH 10.0 and 40°C Cupriavidus necator
1.1.1.91 13.7
-
 (S)-2-octanol isoform SDR A5, at pH 10.0 and 40°C Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.91 6.5
-
 for the reduction reaction Cupriavidus necator
1.1.1.91 11
-
 for the oxidation reaction Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.91 NADP+
-
 Cupriavidus necator
1.1.1.91 NADPH preferred cofactor Cupriavidus necator

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.91 0.6
-
 (S)-2-octanol isoform SDR B3, at pH 10.0 and 40°C Cupriavidus necator
1.1.1.91 1.8
-
 (S)-2-octanol isoform SDR A5, at pH 10.0 and 40°C Cupriavidus necator