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Literature summary extracted from

  • Prabhu, K. A.; R. Maheshwari
    : Biochemical properties of xylanases from a thermophilic fungus, Melanocarpus albomyces, and their action on plant cell walls (1999), J. Biosci., 24, 461-470.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 xylan Km for larchwood xylan 0.33 mg/ml, xylanase IA. Km for larchwood xylan 1.69 mg/ml, xylanase IIIA Melanocarpus albomyces
3.2.1.8 additional information
-
 xylan Km for xylanase IA: 0.33 mg/ml, Km for xylanase IIIA: 1.69 mg/ml Melanocarpus albomyces

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 24000
-
 xylanase IIIA Melanocarpus albomyces
3.2.1.8 38000
-
 xylanase IA Melanocarpus albomyces

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Melanocarpus albomyces
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.8 glycoprotein xlanase IA contains 7% carbohydrate. Xylanase IIIA contains no detectable carbohydrate Melanocarpus albomyces
3.2.1.8 glycoprotein xylanase IA contains 7% carbohydrate, xylanase III contains detectable carbohydrate Melanocarpus albomyces

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 two xylanase activities, a minor xylanase IA and a major xylanase IIIA, are purified Melanocarpus albomyces
3.2.1.8 two xylanase activities, a minor xylanase IA and a major xylanase IIIA, were purified to apparent homogeneity from bagasse-grown cultures Melanocarpus albomyces

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 xylan + H2O xylanase IA and xylanase IIIA are bothe specific for beta-1,4 xylose-rich polymer Melanocarpus albomyces xylobiose + xylotriose + xylooligosaccharides
-
 ?
3.2.1.8 xylan + H2O
-
 Melanocarpus albomyces xylotriose + xylotriose + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 65
-
-
 Melanocarpus albomyces

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 50
-
 stable Melanocarpus albomyces

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5.6
-
 xylanase IIIA Melanocarpus albomyces
3.2.1.8 6.6
-
 xylanase IA Melanocarpus albomyces

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 5 10 50°C, 24 h, stable, xylanase IA and xylanase IIIA Melanocarpus albomyces
3.2.1.8 5 10 50°C, 24, stable Melanocarpus albomyces

Expression

EC Number Organism Comment Expression
3.2.1.8 Melanocarpus albomyces activity is inducible not only by hemicellulosic material but also by the monomeric pentosan unit of xylan but not by glucose up
3.2.1.8 Melanocarpus albomyces xylanase activity is inducible not only by hemicellulosic material but also by the monomeric pentosan unit of xylan but not by glucose up