EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.9 | expressed in Escherichia coli BL21 cells | Gluconobacter oxydans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.9 | 36.4 | - |
NADH | at pH 5.0 and 35°C | Gluconobacter oxydans | |
1.1.1.9 | 43.9 | - |
D-xylulose | at pH 5.0 and 35°C | Gluconobacter oxydans | |
1.1.1.9 | 107.3 | - |
NAD+ | at pH 7.0 and 30°C | Gluconobacter oxydans | |
1.1.1.9 | 175.5 | - |
xylitol | at pH 7.0 and 30°C | Gluconobacter oxydans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | 29000 | - |
x * 29000, SDS-PAGE | Gluconobacter oxydans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.9 | D-xylulose + NADH + H+ | Gluconobacter oxydans | 100% activity | xylitol + NAD+ | - |
r | |
1.1.1.9 | D-xylulose + NADH + H+ | Gluconobacter oxydans CGMCC 1.49 | 100% activity | xylitol + NAD+ | - |
r | |
1.1.1.9 | xylitol + NAD+ | Gluconobacter oxydans | 100% activity | D-xylulose + NADH + H+ | - |
r | |
1.1.1.9 | xylitol + NAD+ | Gluconobacter oxydans CGMCC 1.49 | 100% activity | D-xylulose + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.9 | Gluconobacter oxydans | - |
- |
- |
1.1.1.9 | Gluconobacter oxydans CGMCC 1.49 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.9 | Ni-NTA column chromatography and Sephacryl S-300 gel filtration | Gluconobacter oxydans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.9 | D-fructose + NADH + H+ | 46.4% activity compared to D-xylulose | Gluconobacter oxydans | sorbitol + NAD+ | - |
r | |
1.1.1.9 | D-fructose + NADH + H+ | 46.4% activity compared to D-xylulose | Gluconobacter oxydans CGMCC 1.49 | sorbitol + NAD+ | - |
r | |
1.1.1.9 | D-mannitol + NAD+ | 16.1% activity compared to xylitol | Gluconobacter oxydans | ? | - |
? | |
1.1.1.9 | D-mannitol + NAD+ | 16.1% activity compared to xylitol | Gluconobacter oxydans CGMCC 1.49 | ? | - |
? | |
1.1.1.9 | D-xylulose + NADH + H+ | 100% activity | Gluconobacter oxydans | xylitol + NAD+ | - |
r | |
1.1.1.9 | D-xylulose + NADH + H+ | 100% activity | Gluconobacter oxydans CGMCC 1.49 | xylitol + NAD+ | - |
r | |
1.1.1.9 | sorbitol + NAD+ | 153.9% activity compared to xylitol | Gluconobacter oxydans | D-fructose + NADH + H+ | - |
r | |
1.1.1.9 | sorbitol + NAD+ | 153.9% activity compared to xylitol | Gluconobacter oxydans CGMCC 1.49 | D-fructose + NADH + H+ | - |
r | |
1.1.1.9 | xylitol + NAD+ | 100% activity | Gluconobacter oxydans | D-xylulose + NADH + H+ | - |
r | |
1.1.1.9 | xylitol + NAD+ | 100% activity | Gluconobacter oxydans CGMCC 1.49 | D-xylulose + NADH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.9 | ? | x * 29000, SDS-PAGE | Gluconobacter oxydans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.9 | XDH | - |
Gluconobacter oxydans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | 30 | - |
optimum temperature for the oxidation of xylitol in the presence of coenzyme NAD+ | Gluconobacter oxydans |
1.1.1.9 | 35 | - |
optimum temperature for the for the reduction of D-xylulose in the presence of NADH | Gluconobacter oxydans |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | 25 | 40 | the oxidation and reduction activities of the enzyme on the condition of temperatures ranging from 25°C to 40°C are over 80% | Gluconobacter oxydans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | 5 | - |
optimum pH for the for the reduction of D-xylulose in the presence of NADH | Gluconobacter oxydans |
1.1.1.9 | 11 | - |
optimum pH for the oxidation of xylitol in the presence of coenzyme NAD+ | Gluconobacter oxydans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.9 | NAD+ | - |
Gluconobacter oxydans |