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Literature summary extracted from

  • Qi, X.H.; Zhu, J.F.; Yun, J.H.; Lin, J.; Qi, Y.L.; Guo, Q.; Xu, H.
    Enhanced xylitol production: Expression of xylitol dehydrogenase from Gluconobacter oxydans and mixed culture of resting cell (2016), J. Biosci. Bioeng., 122, 257-262.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.9 expressed in Escherichia coli BL21 cells Gluconobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.9 36.4
-
 NADH at pH 5.0 and 35°C Gluconobacter oxydans
1.1.1.9 43.9
-
 D-xylulose at pH 5.0 and 35°C Gluconobacter oxydans
1.1.1.9 107.3
-
 NAD+ at pH 7.0 and 30°C Gluconobacter oxydans
1.1.1.9 175.5
-
 xylitol at pH 7.0 and 30°C Gluconobacter oxydans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.9 29000
-
 x * 29000, SDS-PAGE Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.9 D-xylulose + NADH + H+ Gluconobacter oxydans 100% activity xylitol + NAD+
-
 r
1.1.1.9 D-xylulose + NADH + H+ Gluconobacter oxydans CGMCC 1.49 100% activity xylitol + NAD+
-
 r
1.1.1.9 xylitol + NAD+ Gluconobacter oxydans 100% activity D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+ Gluconobacter oxydans CGMCC 1.49 100% activity D-xylulose + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.9 Gluconobacter oxydans
-
-
-
1.1.1.9 Gluconobacter oxydans CGMCC 1.49
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.9 Ni-NTA column chromatography and Sephacryl S-300 gel filtration Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.9 D-fructose + NADH + H+ 46.4% activity compared to D-xylulose Gluconobacter oxydans sorbitol + NAD+
-
 r
1.1.1.9 D-fructose + NADH + H+ 46.4% activity compared to D-xylulose Gluconobacter oxydans CGMCC 1.49 sorbitol + NAD+
-
 r
1.1.1.9 D-mannitol + NAD+ 16.1% activity compared to xylitol Gluconobacter oxydans ?
-
 ?
1.1.1.9 D-mannitol + NAD+ 16.1% activity compared to xylitol Gluconobacter oxydans CGMCC 1.49 ?
-
 ?
1.1.1.9 D-xylulose + NADH + H+ 100% activity Gluconobacter oxydans xylitol + NAD+
-
 r
1.1.1.9 D-xylulose + NADH + H+ 100% activity Gluconobacter oxydans CGMCC 1.49 xylitol + NAD+
-
 r
1.1.1.9 sorbitol + NAD+ 153.9% activity compared to xylitol Gluconobacter oxydans D-fructose + NADH + H+
-
 r
1.1.1.9 sorbitol + NAD+ 153.9% activity compared to xylitol Gluconobacter oxydans CGMCC 1.49 D-fructose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+ 100% activity Gluconobacter oxydans D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+ 100% activity Gluconobacter oxydans CGMCC 1.49 D-xylulose + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.9 ? x * 29000, SDS-PAGE Gluconobacter oxydans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.9 XDH
-
 Gluconobacter oxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.9 30
-
 optimum temperature for the oxidation of xylitol in the presence of coenzyme NAD+ Gluconobacter oxydans
1.1.1.9 35
-
 optimum temperature for the for the reduction of D-xylulose in the presence of NADH Gluconobacter oxydans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.9 25 40 the oxidation and reduction activities of the enzyme on the condition of temperatures ranging from 25°C to 40°C are over 80% Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.9 5
-
 optimum pH for the for the reduction of D-xylulose in the presence of NADH Gluconobacter oxydans
1.1.1.9 11
-
 optimum pH for the oxidation of xylitol in the presence of coenzyme NAD+ Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.9 NAD+
-
 Gluconobacter oxydans