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Literature summary extracted from

  • Maalcke, W.J.; Reimann, J.; de Vries, S.; Butt, J.N.; Dietl, A.; Kip, N.; Mersdorf, U.; Barends, T.R.; Jetten, M.S.; Keltjens, J.T.; Kartal, B.
    Characterization of anammox hydrazine dehydrogenase, a key N2-producing enzyme in the global nitrogen cycle (2016), J. Biol. Chem., 291, 17077-17092.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.8 hydroxylamine strong competitive inhibitor Candidatus Kuenenia stuttgartiensis
1.7.2.8 nitric oxide strong competitive inhibitor Candidatus Kuenenia stuttgartiensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.8 0.01
-
 hydrazine at pH 7.0 and 37°C Candidatus Kuenenia stuttgartiensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.8 67000
-
 3 * 67000, SDS-PAGE Candidatus Kuenenia stuttgartiensis
1.7.2.8 200000
-
 gel filtration Candidatus Kuenenia stuttgartiensis
1.7.2.8 201800
-
 MALDI-TOF mass spectrometry Candidatus Kuenenia stuttgartiensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.8 hydrazine + ferricytochrome c Candidatus Kuenenia stuttgartiensis highly specific for hydrazine oxidation activity N2 + ferrocytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.8 Candidatus Kuenenia stuttgartiensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.8 Q Sepharose column chromatography and hydroxyapatite column chromatography Candidatus Kuenenia stuttgartiensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.8 0.55
-
 crude extract, at pH 7.0 and 37°C Candidatus Kuenenia stuttgartiensis
1.7.2.8 8.4
-
 after 15fold purification, at pH 7.0 and 37°C Candidatus Kuenenia stuttgartiensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.8 hydrazine + ferricytochrome c highly specific for hydrazine oxidation activity Candidatus Kuenenia stuttgartiensis N2 + ferrocytochrome c
-
 ?
1.7.2.8 additional information hydroxylamine does not serve as a substrate Candidatus Kuenenia stuttgartiensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.8 homotrimer 3 * 67000, SDS-PAGE Candidatus Kuenenia stuttgartiensis
1.7.2.8 homotrimer 3 * 62272, calculated from amino acid sequence Candidatus Kuenenia stuttgartiensis

Synonyms

EC Number Synonyms Comment Organism
1.7.2.8 HDH
-
 Candidatus Kuenenia stuttgartiensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.2.8 3 6 hydrazine at pH 7.0 and 37°C Candidatus Kuenenia stuttgartiensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.8 heme the enzyme possessesg eight bound heme molecules Candidatus Kuenenia stuttgartiensis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.2.8 0.0025
-
 nitric oxide at pH 7.0 and 37°C Candidatus Kuenenia stuttgartiensis
1.7.2.8 0.0079
-
 hydroxylamine at pH 7.0 and 37°C Candidatus Kuenenia stuttgartiensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7.2.8 3500
-
 hydrazine at pH 7.0 and 37°C Candidatus Kuenenia stuttgartiensis