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Literature summary extracted from

  • Rodionova, I.A.; Zuccola, H.J.; Sorci, L.; Aleshin, A.E.; Kazanov, M.D.; Ma, C.T.; Sergienko, E.; Rubin, E.J.; Locher, C.P.; Osterman, A.L.
    Mycobacterial nicotinate mononucleotide adenylyltransferase: structure, mechanism, and implications for drug discovery (2015), J. Biol. Chem., 290, 7693-7706.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.7.18 drug development enzyme NadD is a potentially druggable target suitable for the development of selective inhibitors Mycobacterium tuberculosis
2.7.7.18 medicine the enzyme is a target for developing antimycobacterial therapies Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.18 gene nadD, sequence comparisons, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.18 purified recombinant wild-type and W117A mutant enzymes, sitting drop vapour diffusion method at 20°C, microcrystal seeding using hanging drops, mixing of 10 mg/ml protein in 20 mM Tris, pH 7.8, 200 mM NaCl, and 5% v/v glycerol with well solution containing 1.2-1.5 M MgSO4, 0.1 M MES buffer, pH 6.0-6.5, microseeds are added to the drops 1 h later, macrocrystals growth within 3-5 days., X-ray diffraction structure determination and analysis at 2.4 A resolution, molecular replacement Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.18 D14A site-directed mutagenesis, almost inactive mutant Mycobacterium tuberculosis
2.7.7.18 H17A site-directed mutagenesis, almost inactive mutant Mycobacterium tuberculosis
2.7.7.18 H20A site-directed mutagenesis, almost inactive mutant Mycobacterium tuberculosis
2.7.7.18 K47A site-directed mutagenesis, the mutant shows a decrease in Km for ATP and a 6fold increase in Km for nicotinate beta-D-ribonucleotide and a nearly abolished enzyme activity Mycobacterium tuberculosis
2.7.7.18 L164A site-directed mutagenesis, the L164A mutant elutes as both a monomer and a dimer during purification, the mutant shows 40fold reduced activity compared to the wild-type enzyme Mycobacterium tuberculosis
2.7.7.18 L164Q site-directed mutagenesis, the L164Q mutant elutes as both a monomer and a dimer during purification, almost inactive mutant Mycobacterium tuberculosis
2.7.7.18 P44A site-directed mutagenesis, the mutant shows a decrease in Km for ATP Mycobacterium tuberculosis
2.7.7.18 Q46A site-directed mutagenesis Mycobacterium tuberculosis
2.7.7.18 T12A site-directed mutagenesis, the T12A mutant elutes as a dimer and as an aggregated protein during purification, the mutant shows highly reduced activity compared to the wild-type enzyme Mycobacterium tuberculosis
2.7.7.18 T86A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Mycobacterium tuberculosis
2.7.7.18 W117A site-directed mutagenesis, the mutant shows a 10fold higher expression level compared to the wild-type enzyme in Escherichia coli, three-dimensional structure determination and analysis, the mutant shows highly reduced activity compared to the wild-type enzyme Mycobacterium tuberculosis
2.7.7.18 W117F site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Mycobacterium tuberculosis
2.7.7.18 W45A site-directed mutagenesis Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.18 2-(2,4-dimethylphenyl)-N-(4-([(5-methyl-3-isoxazolyl)amino]sulfonyl)phenyl)-4-quinolinecarboxamide MIC80 values of 0.02-0.1 mM and above. The compounds show no inhibition of the human enzyme Mycobacterium tuberculosis
2.7.7.18 2-(2,4-dimethylphenyl)-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide
-
 Mycobacterium tuberculosis
2.7.7.18 2-(2,4-dimethylphenyl)-N-[4-[(5-methyl-1,2-oxazol-3-yl)sulfamoyl]phenyl]quinoline-4-carboxamide
-
 Mycobacterium tuberculosis
2.7.7.18 2-(2-chlorophenyl)-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide
-
 Mycobacterium tuberculosis
2.7.7.18 2-(4-bromophenyl)-N-[4-[(4-methylpyrimidin-2-yl)sulfamoyl]phenyl]quinoline-4-carboxamide
-
 Mycobacterium tuberculosis
2.7.7.18 2-(furan-2-yl)-N-[3-(3,4,5,6-tetrahydropyridin-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide
-
 Mycobacterium tuberculosis
2.7.7.18 2-(furan-2-yl)-N-[3-methoxy-4-(morpholin-4-ylsulfonyl)phenyl]quinoline-4-carboxamide
-
 Mycobacterium tuberculosis
2.7.7.18 2-(furan-2-yl)-N-[3-methyl-4-(morpholin-4-ylsulfonyl)phenyl]quinoline-4-carboxamide
-
 Mycobacterium tuberculosis
2.7.7.18 2-(pyridin-4-yl)-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide
-
 Mycobacterium tuberculosis
2.7.7.18 2-phenyl-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide
-
 Mycobacterium tuberculosis
2.7.7.18 3-(1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]benzamide
-
 Mycobacterium tuberculosis
2.7.7.18 4-[ethyl(phenyl)sulfamoyl]-N-[5-(2-methoxyphenyl)-1,3,4-oxadiazol-2-yl]benzamide
-
 Mycobacterium tuberculosis
2.7.7.18 additional information inhibitory profiling of partially active mutants and wild-type enzyme MtNadD using small molecule inhibitors, overview. Substrate inhibition in an ordered bi-reactant system Mycobacterium tuberculosis
2.7.7.18 N-[3-(3,4-dihydro-2H-pyrrol-5-ylsulfamoyl)phenyl]-2-(furan-2-yl)quinoline-4-carboxamide
-
 Mycobacterium tuberculosis
2.7.7.18 N-[3-[1-(6-chloro-4-phenylquinazolin-2-yl)-5-(furan-2-yl)-4,5-dihydro-1H-pyrazol-3-yl]phenyl]methanesulfonamide
-
 Mycobacterium tuberculosis
2.7.7.18 N-[4-(3,4-dihydro-2H-pyrrol-5-ylsulfamoyl)phenyl]-2-(furan-2-yl)quinoline-4-carboxamide
-
 Mycobacterium tuberculosis
2.7.7.18 N-[4-(4-iodophenyl)-1,3-thiazol-2-yl]-3-[(phenylsulfonyl)amino]benzamide
-
 Mycobacterium tuberculosis
2.7.7.18 N-[4-(4-methoxyphenyl)-1,3-thiazol-2-yl]-3-[(phenylsulfonyl)amino]benzamide
-
 Mycobacterium tuberculosis
2.7.7.18 N-[4-[(3,4-dimethyl-1,2-oxazol-5-yl)sulfamoyl]-3-methylphenyl]-2-(4-methoxyphenyl)quinoline-4-carboxamide
-
 Mycobacterium tuberculosis
2.7.7.18 N-[4-[(3,4-dimethyl-1,2-oxazol-5-yl)sulfamoyl]-3-methylphenyl]-2-(4-methylphenyl)quinoline-4-carboxamide
-
 Mycobacterium tuberculosis
2.7.7.18 S-(4-bromo-8-chloronaphthalen-1-yl) [4-(1,3-thiazol-2-ylsulfamoyl)phenyl]carbamothioate
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.18 additional information
-
 additional information steady-state kinetic analysis, sequential kinetic mechanism, bisubstrate enzyme kinetics, overview Mycobacterium tuberculosis
2.7.7.18 0.006
-
 ATP pH 7.5, 37°C, recombinant mutant K47A Mycobacterium tuberculosis
2.7.7.18 0.011
-
 ATP pH 7.5, 37°C, recombinant mutant T86A Mycobacterium tuberculosis
2.7.7.18 0.011
-
 ATP pH 7.5, 37°C, recombinant mutant W45A Mycobacterium tuberculosis
2.7.7.18 0.017
-
 ATP pH 7.5, 37°C, recombinant mutant P44A Mycobacterium tuberculosis
2.7.7.18 0.023
-
 ATP pH 7.5, 37°C, recombinant mutant W117F Mycobacterium tuberculosis
2.7.7.18 0.033
-
 ATP pH 7.5, 37°C, recombinant mutant Q46A Mycobacterium tuberculosis
2.7.7.18 0.041
-
 ATP pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis
2.7.7.18 0.042
-
 ATP pH 7.5, 37°C, recombinant mutant T12A Mycobacterium tuberculosis
2.7.7.18 0.17
-
 ATP pH 7.5, 37°C, recombinant mutant L164A Mycobacterium tuberculosis
2.7.7.18 0.19
-
 ATP pH 7.5, 37°C, recombinant mutant L164Q Mycobacterium tuberculosis
2.7.7.18 0.33
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant Q46A Mycobacterium tuberculosis
2.7.7.18 0.43
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant L164Q Mycobacterium tuberculosis
2.7.7.18 0.48
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis
2.7.7.18 0.52
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant L164A Mycobacterium tuberculosis
2.7.7.18 0.53
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant T12A Mycobacterium tuberculosis
2.7.7.18 0.84
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant P44A Mycobacterium tuberculosis
2.7.7.18 0.98
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant W45A Mycobacterium tuberculosis
2.7.7.18 1.13
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant W117F Mycobacterium tuberculosis
2.7.7.18 2.67
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant W117A Mycobacterium tuberculosis
2.7.7.18 2.7
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant K47A Mycobacterium tuberculosis
2.7.7.18 2.8
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant T86A Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.18 Mg2+ required Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.18 27000
-
 2 * 27000, SDS-PAGE Mycobacterium tuberculosis
2.7.7.18 54000
-
 about, gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.18 ATP + nicotinate beta-D-ribonucleotide Mycobacterium tuberculosis
-
 diphosphate + deamido-NAD+
-
 ?
2.7.7.18 ATP + nicotinate beta-D-ribonucleotide Mycobacterium tuberculosis H37Rv
-
 diphosphate + deamido-NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.18 Mycobacterium tuberculosis P9WJJ5 gene nadD
-
2.7.7.18 Mycobacterium tuberculosis H37Rv P9WJJ5 gene nadD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.18 recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage from wild-type enzyme and W117A mutant enzyme through Ulp1 protease or TEV protease, and anion exchange chromatography and dialysis Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.18 ATP + nicotinate beta-D-ribonucleotide
-
 Mycobacterium tuberculosis diphosphate + deamido-NAD+
-
 ?
2.7.7.18 ATP + nicotinate beta-D-ribonucleotide
-
 Mycobacterium tuberculosis H37Rv diphosphate + deamido-NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.18 homodimer 2 * 27000, SDS-PAGE Mycobacterium tuberculosis
2.7.7.18 More three-dimensional structure enzyme structure determination and analysis, crystal structure analysis Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.7.7.18 nadD
-
 Mycobacterium tuberculosis
2.7.7.18 nicotinate mononucleotide adenylyltransferase
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.18 37
-
 assay at Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.18 0.002
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant W117A Mycobacterium tuberculosis
2.7.7.18 0.015
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant L164Q Mycobacterium tuberculosis
2.7.7.18 0.02
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant K47A Mycobacterium tuberculosis
2.7.7.18 0.03
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant W117F Mycobacterium tuberculosis
2.7.7.18 0.13
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant L164A Mycobacterium tuberculosis
2.7.7.18 0.2
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant T86A Mycobacterium tuberculosis
2.7.7.18 0.22
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant T12A Mycobacterium tuberculosis
2.7.7.18 0.36
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant P44A Mycobacterium tuberculosis
2.7.7.18 0.37
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant W45A Mycobacterium tuberculosis
2.7.7.18 4.7
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant Q46A Mycobacterium tuberculosis
2.7.7.18 5.5
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.18 7.5
-
 assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.7.18 ATP binding site structure, overview Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.18 additional information
-
 additional information inhibition kinetics, overview Mycobacterium tuberculosis
2.7.7.18 0.024
-
 2-(2,4-dimethylphenyl)-N-(4-([(5-methyl-3-isoxazolyl)amino]sulfonyl)phenyl)-4-quinolinecarboxamide pH 7.5, 37°C, recombinant mutant T12A Mycobacterium tuberculosis
2.7.7.18 0.03
-
 2-(2,4-dimethylphenyl)-N-(4-([(5-methyl-3-isoxazolyl)amino]sulfonyl)phenyl)-4-quinolinecarboxamide pH 7.5, 37°C, recombinant mutant W117A Mycobacterium tuberculosis
2.7.7.18 0.035
-
 2-(2,4-dimethylphenyl)-N-(4-([(5-methyl-3-isoxazolyl)amino]sulfonyl)phenyl)-4-quinolinecarboxamide pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis
2.7.7.18 0.037
-
 2-(2,4-dimethylphenyl)-N-(4-([(5-methyl-3-isoxazolyl)amino]sulfonyl)phenyl)-4-quinolinecarboxamide pH 7.5, 37°C, recombinant mutant P44A Mycobacterium tuberculosis
2.7.7.18 0.056
-
 2-(2,4-dimethylphenyl)-N-(4-([(5-methyl-3-isoxazolyl)amino]sulfonyl)phenyl)-4-quinolinecarboxamide pH 7.5, 37°C, recombinant mutant W45A Mycobacterium tuberculosis
2.7.7.18 0.094
-
 2-(2,4-dimethylphenyl)-N-(4-([(5-methyl-3-isoxazolyl)amino]sulfonyl)phenyl)-4-quinolinecarboxamide pH 7.5, 37°C, recombinant mutant K47A Mycobacterium tuberculosis
2.7.7.18 0.114
-
 2-(2,4-dimethylphenyl)-N-(4-([(5-methyl-3-isoxazolyl)amino]sulfonyl)phenyl)-4-quinolinecarboxamide pH 7.5, 37°C, recombinant mutant T86A Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.7.18 0.017
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis 2-(furan-2-yl)-N-[3-(3,4,5,6-tetrahydropyridin-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide
2.7.7.18 0.024
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis 3-(1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]benzamide
2.7.7.18 0.024
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis S-(4-bromo-8-chloronaphthalen-1-yl) [4-(1,3-thiazol-2-ylsulfamoyl)phenyl]carbamothioate
2.7.7.18 0.033
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis 2-(2-chlorophenyl)-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide
2.7.7.18 0.035
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis 2-(2,4-dimethylphenyl)-N-[4-[(5-methyl-1,2-oxazol-3-yl)sulfamoyl]phenyl]quinoline-4-carboxamide
2.7.7.18 0.036
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis 2-(2,4-dimethylphenyl)-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide
2.7.7.18 0.043
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis 2-phenyl-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide
2.7.7.18 0.046
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis N-[4-(3,4-dihydro-2H-pyrrol-5-ylsulfamoyl)phenyl]-2-(furan-2-yl)quinoline-4-carboxamide
2.7.7.18 0.049
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis N-[3-(3,4-dihydro-2H-pyrrol-5-ylsulfamoyl)phenyl]-2-(furan-2-yl)quinoline-4-carboxamide
2.7.7.18 0.049
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis 2-(4-bromophenyl)-N-[4-[(4-methylpyrimidin-2-yl)sulfamoyl]phenyl]quinoline-4-carboxamide
2.7.7.18 0.051
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis 2-(furan-2-yl)-N-[3-methyl-4-(morpholin-4-ylsulfonyl)phenyl]quinoline-4-carboxamide
2.7.7.18 0.056
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis 2-(pyridin-4-yl)-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide
2.7.7.18 0.074
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis 2-(furan-2-yl)-N-[3-methoxy-4-(morpholin-4-ylsulfonyl)phenyl]quinoline-4-carboxamide
2.7.7.18 0.096
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis 4-[ethyl(phenyl)sulfamoyl]-N-[5-(2-methoxyphenyl)-1,3,4-oxadiazol-2-yl]benzamide
2.7.7.18 0.116
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis N-[4-(4-methoxyphenyl)-1,3-thiazol-2-yl]-3-[(phenylsulfonyl)amino]benzamide
2.7.7.18 0.13
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis N-[4-[(3,4-dimethyl-1,2-oxazol-5-yl)sulfamoyl]-3-methylphenyl]-2-(4-methylphenyl)quinoline-4-carboxamide
2.7.7.18 0.13
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis N-[4-[(3,4-dimethyl-1,2-oxazol-5-yl)sulfamoyl]-3-methylphenyl]-2-(4-methoxyphenyl)quinoline-4-carboxamide
2.7.7.18 0.13
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis N-[3-[1-(6-chloro-4-phenylquinazolin-2-yl)-5-(furan-2-yl)-4,5-dihydro-1H-pyrazol-3-yl]phenyl]methanesulfonamide
2.7.7.18 0.18
-
 pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis N-[4-(4-iodophenyl)-1,3-thiazol-2-yl]-3-[(phenylsulfonyl)amino]benzamide

General Information

EC Number General Information Comment Organism
2.7.7.18 additional information the enzyme has a unique 310 helix that locks the active site in an over-closed conformation. This renders the enzyme inactive as it is topologically incompatible with substrate binding and catalysis. The enzyme contains the signature motifs GXFXPX(H/T)XXH and SXTXXR mainly implicated in ATP binding. Mechanistic modelling, overview Mycobacterium tuberculosis
2.7.7.18 physiological function nicotinate mononucleotide adenylyltransferase NadD is an essential enzyme in the biosynthesis of the NAD+ cofactor Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.18 0.0008
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant W117A Mycobacterium tuberculosis
2.7.7.18 0.007
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant K47A Mycobacterium tuberculosis
2.7.7.18 0.008
-
 ATP pH 7.5, 37°C, recombinant mutant L164Q Mycobacterium tuberculosis
2.7.7.18 0.03
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant W117F Mycobacterium tuberculosis
2.7.7.18 0.04
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant L164Q Mycobacterium tuberculosis
2.7.7.18 0.07
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant T86A Mycobacterium tuberculosis
2.7.7.18 0.08
-
 ATP pH 7.5, 37°C, recombinant mutant L164A Mycobacterium tuberculosis
2.7.7.18 0.4
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant P44A Mycobacterium tuberculosis
2.7.7.18 0.4
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant T12A Mycobacterium tuberculosis
2.7.7.18 0.4
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant W45A Mycobacterium tuberculosis
2.7.7.18 1.3
-
 ATP pH 7.5, 37°C, recombinant mutant W117F Mycobacterium tuberculosis
2.7.7.18 2.5
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant L164A Mycobacterium tuberculosis
2.7.7.18 3
-
 ATP pH 7.5, 37°C, recombinant mutant K47A Mycobacterium tuberculosis
2.7.7.18 5
-
 ATP pH 7.5, 37°C, recombinant mutant T12A Mycobacterium tuberculosis
2.7.7.18 12
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis
2.7.7.18 14
-
 nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant Q46A Mycobacterium tuberculosis
2.7.7.18 18
-
 ATP pH 7.5, 37°C, recombinant mutant T86A Mycobacterium tuberculosis
2.7.7.18 21
-
 ATP pH 7.5, 37°C, recombinant mutant P44A Mycobacterium tuberculosis
2.7.7.18 34
-
 ATP pH 7.5, 37°C, recombinant mutant W45A Mycobacterium tuberculosis
2.7.7.18 134
-
 ATP pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis
2.7.7.18 142
-
 ATP pH 7.5, 37°C, recombinant mutant Q46A Mycobacterium tuberculosis