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Literature summary extracted from

  • Cuskin, F.; Basle, A.; Ladeveze, S.; Day, A.; Gilbert, H.; Davies, G.; Potocki-Veronese, G.; Lowe, E.
    The GH130 family of mannoside phosphorylases contains glycoside hydrolases that target beta-1,2-mannosidic linkages in Candida mannan (2015), J. Biol. Chem., 290, 25023-25033.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.197 in complex with mannose bound in the -1 and +1 subsites. A pair of glutamate residues, Glu227 and Glu268, hydrogen bond to O1 of alpha-mannose, and either of these residues may function as the catalytic base. Conserved lysine residue, Lys199 is a key specificity determinant for beta-1,2-mannosidic linkages Bacteroides thetaiotaomicron

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.197 D142N no activity Bacteroides thetaiotaomicron
3.2.1.197 D363A no activity Bacteroides thetaiotaomicron
3.2.1.197 E141Q no activity Bacteroides thetaiotaomicron
3.2.1.197 E227Q 32% of wild-type activity Bacteroides thetaiotaomicron
3.2.1.197 E227Q/E268Q no activity Bacteroides thetaiotaomicron
3.2.1.197 E268A 5% of wild-type activity Bacteroides thetaiotaomicron
3.2.1.197 E268Q 4% of wild-type activity Bacteroides thetaiotaomicron
3.2.1.197 F344A 31% of wild-type activity Bacteroides thetaiotaomicron
3.2.1.197 K199A no activity Bacteroides thetaiotaomicron
3.2.1.197 N74A 15% of wild-type activity Bacteroides thetaiotaomicron
3.2.1.197 R89A 25% of wild-type activity Bacteroides thetaiotaomicron
3.2.1.197 Y302A 6% of wild-type activity Bacteroides thetaiotaomicron

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.197 Bacteroides ovatus A7M0J4
-
-
3.2.1.197 Bacteroides ovatus DSM 1896 A7M0J4
-
-
3.2.1.197 Bacteroides thetaiotaomicron Q8A186
-
-
3.2.1.197 Bacteroides thetaiotaomicron DSM 2079 Q8A186
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.197 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
-
 Bacteroides thetaiotaomicron beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
-
 ?
3.2.1.197 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
-
 ?
3.2.1.197 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
-
 Bacteroides thetaiotaomicron beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose enzyme releases alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration ?
3.2.1.197 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose enzyme releases alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration ?
3.2.1.197 beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
-
 Bacteroides thetaiotaomicron beta-D-mannopyranose + alpha-D-mannopyranose
-
 ?
3.2.1.197 beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 beta-D-mannopyranose + alpha-D-mannopyranose
-
 ?
3.2.1.197 Candida mannan + H2O
-
 Bacteroides thetaiotaomicron ? + alpha-D-mannopyranose
-
 ?
3.2.1.197 Candida mannan + H2O
-
 Bacteroides ovatus ? + alpha-D-mannopyranose
-
 ?
3.2.1.197 Candida mannan + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 ? + alpha-D-mannopyranose
-
 ?
3.2.1.197 Candida mannan + H2O
-
 Bacteroides ovatus DSM 1896 ? + alpha-D-mannopyranose
-
 ?
3.2.1.197 additional information enzyme is a beta-mannosidase that hydrolyzes beta-1,2-mannosidic linkages through an inverting mechanism Bacteroides thetaiotaomicron ?
-
 ?
3.2.1.197 additional information enzyme is a beta-mannosidase that hydrolyzes beta-1,2-mannosidic linkages through an inverting mechanism Bacteroides thetaiotaomicron DSM 2079 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.197 BACOVA_03624
-
 Bacteroides ovatus
3.2.1.197 BT_3780
-
 Bacteroides thetaiotaomicron

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.197 0.04
-
 beta-D-mannopyranosyl-(1->2)-D-mannopyranose pH 7.5., 37°C Bacteroides thetaiotaomicron
3.2.1.197 0.06
-
 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose pH 7.5., 37°C Bacteroides thetaiotaomicron
3.2.1.197 0.085
-
 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose pH 7.5., 37°C Bacteroides thetaiotaomicron
3.2.1.197 0.1
-
 Candida mannan pH 7.5., 37°C Bacteroides ovatus
3.2.1.197 0.115
-
 Candida mannan pH 7.5., 37°C Bacteroides thetaiotaomicron