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Literature summary extracted from

  • Yang, Y.; Ye, Q.; Jia, Z.; Cote, G.P.
    Characterization of the catalytic and nucleotide binding properties of the alpha-kinase domain of Dictyostelium myosin-II heavy chain kinase A (2015), J. Biol. Chem., 290, 23935-23946.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.7 expressed in Escherichia coli BL21(DE3) cells Dictyostelium discoideum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.11.7 alpha-kinase domain in complex with AMP and adenosine, hanging drop vapor diffusion method, using 200 mM NaH2PO4, 20% (w/v) polyethylene glycol 8000, and 100 mM Tris-HCl, pH 7.5 Dictyostelium discoideum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.7 D663A the mutant shows about 3% activity compared to the wild type enzyme Dictyostelium discoideum
2.7.11.7 D756A inactive Dictyostelium discoideum
2.7.11.7 D766S inactive Dictyostelium discoideum
2.7.11.7 E713A the mutant shows about 10% activity compared to the wild type enzyme Dictyostelium discoideum
2.7.11.7 E713D the mutant shows about 3% activity compared to the wild type enzyme Dictyostelium discoideum
2.7.11.7 F720S inactive Dictyostelium discoideum
2.7.11.7 K645R the mutant shows about 3% activity compared to the wild type enzyme Dictyostelium discoideum
2.7.11.7 K722N the mutant shows about 20% activity compared to the wild type enzyme Dictyostelium discoideum
2.7.11.7 L716S the mutant shows about 1% activity compared to the wild type enzyme Dictyostelium discoideum
2.7.11.7 Q758A the mutant shows about 3% activity compared to the wild type enzyme Dictyostelium discoideum
2.7.11.7 Q768A the mutant shows about 70% activity compared to the wild type enzyme Dictyostelium discoideum
2.7.11.7 R592L the mutant shows about 30% activity compared to the wild type enzyme Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.7 0.058
-
 ATP mutant enzyme R592L, at pH 7.0 and 22°C Dictyostelium discoideum
2.7.11.7 0.063
-
 ATP mutant enzyme K722N, at pH 7.0 and 22°C Dictyostelium discoideum
2.7.11.7 0.23
-
 ATP wild type enzyme, at pH 7.0 and 22°C Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.7 ATP + [myosin heavy-chain] Dictyostelium discoideum
-
 ADP + [myosin heavy-chain] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.7 Dictyostelium discoideum P42527
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.7 DE53 column chromatography and SP-Sepharose column chromatography Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.7 ATP + AAYKTKKKK
-
 Dictyostelium discoideum ?
-
 ?
2.7.11.7 ATP + myelin basic protein
-
 Dictyostelium discoideum ?
-
 ?
2.7.11.7 ATP + RKKFGESEKTKTKEFL
-
 Dictyostelium discoideum ?
-
 ?
2.7.11.7 ATP + [myosin heavy-chain]
-
 Dictyostelium discoideum ADP + [myosin heavy-chain] phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.7 A-CAT alpha-kinase domain of myosin II heavy chain kinase A Dictyostelium discoideum
2.7.11.7 MHCK-A
-
 Dictyostelium discoideum
2.7.11.7 myosin II heavy chain kinase A
-
 Dictyostelium discoideum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.11.7 0.017
-
 ATP mutant enzyme K722N, at pH 7.0 and 22°C Dictyostelium discoideum
2.7.11.7 0.03
-
 ATP mutant enzyme R592L, at pH 7.0 and 22°C Dictyostelium discoideum
2.7.11.7 0.1
-
 ATP wild type enzyme, at pH 7.0 and 22°C Dictyostelium discoideum