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Literature summary extracted from

  • Fan, A.; Zocher, G.; Stec, E.; Stehle, T.; Li, S.M.
    Site-directed mutagenesis switching a dimethylallyl tryptophan synthase to a specific tyrosine C3-prenylating enzyme (2015), J. Biol. Chem., 290, 1364-1373.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.B31 recombinant overexpression Aspergillus fumigatus
2.5.1.34 gene 4-DMTAS, recombinant overexpression in Escherichia coli strain BL21 (DE3)pLysS Aspergillus fumigatus

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.34 E89A site-directed mutagenesis Aspergillus fumigatus
2.5.1.34 I80F site-directed mutagenesis Aspergillus fumigatus
2.5.1.34 K174E site-directed mutagenesis Aspergillus fumigatus
2.5.1.34 K174F site-directed mutagenesis, the FgaPT2 mutant shows a much higher catalytic activity toward L-tyrosine than L-tryptophan compared to the wild-type. The single mutation on the key amino acid switches the tryptophan C4-prenyltransferase to a tyrosine C3-prenylating enzyme Aspergillus fumigatus
2.5.1.34 K174F/R244E site-directed mutagenesis Aspergillus fumigatus
2.5.1.34 K174Q site-directed mutagenesis Aspergillus fumigatus
2.5.1.34 K174W site-directed mutagenesis Aspergillus fumigatus
2.5.1.34 K174Y site-directed mutagenesis Aspergillus fumigatus
2.5.1.34 additional information inhibitor screening for L-Tyr prenylation activity, overview Aspergillus fumigatus
2.5.1.34 R244D site-directed mutagenesis Aspergillus fumigatus
2.5.1.34 R244E site-directed mutagenesis Aspergillus fumigatus
2.5.1.34 R244N site-directed mutagenesis Aspergillus fumigatus
2.5.1.34 R244Q site-directed mutagenesis Aspergillus fumigatus
2.5.1.34 T102C site-directed mutagenesis Aspergillus fumigatus
2.5.1.34 T102G site-directed mutagenesis Aspergillus fumigatus
2.5.1.34 T102R site-directed mutagenesis Aspergillus fumigatus
2.5.1.34 T102S site-directed mutagenesis Aspergillus fumigatus
2.5.1.34 T102V site-directed mutagenesis Aspergillus fumigatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.B31 Ca2+ required Aspergillus fumigatus
2.5.1.34 Ca2+ required Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.B31 dimethylallyl diphosphate + L-tryptophan Aspergillus fumigatus
-
 diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan Aspergillus fumigatus
-
 diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.80 dimethylallyl diphosphate + L-tryptophan Aspergillus fumigatus
-
 diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.122 dimethylallyl diphosphate + L-tyrosine Aspergillus fumigatus
-
 diphosphate + 4-O-dimethylallyl-L-tyrosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.B31 Aspergillus fumigatus
-
-
-
2.5.1.34 Aspergillus fumigatus Q50EL0 gene 4-DMATS
-
2.5.1.80 Aspergillus fumigatus Q4WYG3
-
-
2.5.1.122 Aspergillus fumigatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.B31 recombinant enzyme Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.B31 dimethylallyl diphosphate + L-tryptophan
-
 Aspergillus fumigatus diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.B31 additional information no activity with L-tyrosine Aspergillus fumigatus ?
-
 ?
2.5.1.34 dimethylallyl diphosphate + 4-amino-L-phenylalanine low activity with the wild-type enzyme, higher activity with the enzyme mutant K174F Aspergillus fumigatus diphosphate + 4-amino-3-(3-methylbut-2-enyl)-L-phenylalanine
-
 ?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan
-
 Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan preferred enzyme of the wild-type enzyme Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.34 dimethylallyl diphosphate + L-tyrosine low activity with the wild-type enzyme, higher activity with the enzyme mutant K174F Aspergillus fumigatus diphosphate + 3-(3-methylbut-2-enyl)-L-tyrosine
-
 ?
2.5.1.34 additional information the enzyme catalyzes the C-4 prenylation of L-tryptophan, and also the C-3 prenylation of L-tyrosine, cf. EC 2.5.1.122. Single mutation on the key amino acid switches the tryptophan C4 prenyltransferase to a tyrosine C3-prenylating enzyme, active sites and proposed reaction model of FgaPT2 with substrates L-Tyr and 4-amino-L-phenylalanine, overview Aspergillus fumigatus ?
-
 ?
2.5.1.80 dimethylallyl diphosphate + L-tryptophan
-
 Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.80 dimethylallyl diphosphate + L-tryptophan the enzyme catalyzes C7-prenylation of the indole ring Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.80 additional information enzyme 7-DMATS also accepts L-tyrosine as substrate and converts it to an O-prenylated derivative, cf. EC 2.5.1.122. 7-DMATS is also able to take L-tyrosine and two derivatives thereof as substrates and catalyzes the same O- or N-prenylation Aspergillus fumigatus ?
-
 ?
2.5.1.122 dimethylallyl diphosphate + L-tyrosine
-
 Aspergillus fumigatus diphosphate + 4-O-dimethylallyl-L-tyrosine
-
 ?
2.5.1.122 additional information the enzyme catalyzes C7-prenylation of the indole ring, EC 2.5.1.80, but also accepts L-tyrosine as substrate and converts it to an O-prenylated derivative, EC 2.5.1.122. 7-DMATS is able to take L-tyrosine and two derivatives thereof as substrates and catalyzed the same O- or N-prenylation Aspergillus fumigatus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.B31 5-DMATS
-
 Aspergillus fumigatus
2.5.1.34 4-DMATS
-
 Aspergillus fumigatus
2.5.1.34 FgaPT2
-
 Aspergillus fumigatus
2.5.1.80 7-dimethylallyl tryptophan synthase
-
 Aspergillus fumigatus
2.5.1.80 7-DMATS
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.B31 37
-
 assay at Aspergillus fumigatus
2.5.1.34 37
-
 assay at Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.B31 7.5
-
 assay at Aspergillus fumigatus
2.5.1.34 7.5
-
 assay at Aspergillus fumigatus