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Literature summary extracted from

  • Merceron, R.; Awama, A.M.; Montserret, R.; Marcillat, O.; Gouet, P.
    The substrate-free and -bound crystal structures of the duplicated taurocyamine kinase from the human parasite Schistosoma mansoni (2015), J. Biol. Chem., 290, 12951-12963.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.4 recombinant overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Schistosoma mansoni

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.3.4 purified recombinant wild-type and mutant enzymes complexed with taurocyamine or L-arginine, sitting drop vapor diffusion method, mixing of 5 mg/ml protein in 20mM Tris-HCl, pH 8.5, with reservoir solution, containing 200mM diammonium tartrate, pH 5.4, 20% w/v PEG 3350, 20% v/v ethylene glycol, in a 2:1 or 1:1 protein/solution volume ratio, 2 weeks, 20°C, X-ray diffraction structure determination and analysis at 2.2 A resolution leads to a small angle x-ray scattering model of SmTK-TSA in solution with two closed active sites, molecular replacement. The SmTK crystal is soaked with the dead end transition state analogue components taurocyamine-NO3 2-MgADP Schistosoma mansoni

Protein Variants

EC Number Protein Variants Comment Organism
2.7.3.4 C268S site-directed mutagenesis Schistosoma mansoni
2.7.3.4 C268S/C631S site-directed mutagenesis Schistosoma mansoni
2.7.3.4 C631S site-directed mutagenesis Schistosoma mansoni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.3.4 Mg2+ dependent on Schistosoma mansoni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.3.4 ATP + taurocyamine Schistosoma mansoni
-
 ADP + N-phosphotaurocyamine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.4 Schistosoma mansoni P16641
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.4 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by Blue Sepharose affinity and anion exchange chromatography, followed by ultrafiltration Schistosoma mansoni

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.3.4 ATP + taurocyamine = ADP + N-phosphotaurocyamine an arginine residue of the phosphagen specificity loop is crucial for substrate specificity, mechanism and structure-function relationship, overview Schistosoma mansoni
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.4 ATP + taurocyamine
-
 Schistosoma mansoni ADP + N-phosphotaurocyamine
-
 r
2.7.3.4 ATP + taurocyamine the enzyme catalyzes the reversibleMg2-dependent transfer of a phosphoryl group between ATP and taurocyamine Schistosoma mansoni ADP + N-phosphotaurocyamine
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.3.4 More the two unliganded lobes present a canonical open conformation and interact via their respective C- and N-terminal domains at a helix-mediated interface. The two lobes function independently Schistosoma mansoni

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.3.4 ADP
-
 Schistosoma mansoni
2.7.3.4 ATP
-
 Schistosoma mansoni

General Information

EC Number General Information Comment Organism
2.7.3.4 evolution the enzyme belongs to the phosphagen kinase (PK) family. Schistosoma mansoni taurocyamine SmTK is derived from gene duplication, as are all known trematode taurocyamine kinases Schistosoma mansoni