EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.9.1 | additional information | construction of a PPDK deletion mutant, DELTAppdk, and a DELTAppdk/DELTApepck double deletion mutant | Trypanosoma brucei |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.9.1 | glycosome | - |
Trypanosoma brucei | 20015 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.9.1 | Mg2+ | required | Trypanosoma brucei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.9.1 | ATP + pyruvate + phosphate | Trypanosoma brucei | PPDK functions in the glycolytic direction with production of ATP in the glycosomes | AMP + phosphoenolpyruvate + diphosphate | - |
r | |
2.7.9.1 | ATP + pyruvate + phosphate | Trypanosoma brucei EATRO1125.T7T and 427 90-13 | PPDK functions in the glycolytic direction with production of ATP in the glycosomes | AMP + phosphoenolpyruvate + diphosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.9.1 | Trypanosoma brucei | O76283 | - |
- |
2.7.9.1 | Trypanosoma brucei EATRO1125.T7T and 427 90-13 | O76283 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.9.1 | bloodstream form | 427 90-13 | Trypanosoma brucei | - |
2.7.9.1 | procyclic form | EATRO1125.T7T | Trypanosoma brucei | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.9.1 | ATP + pyruvate + phosphate | - |
Trypanosoma brucei | AMP + phosphoenolpyruvate + diphosphate | - |
r | |
2.7.9.1 | ATP + pyruvate + phosphate | PPDK functions in the glycolytic direction with production of ATP in the glycosomes | Trypanosoma brucei | AMP + phosphoenolpyruvate + diphosphate | - |
r | |
2.7.9.1 | ATP + pyruvate + phosphate | - |
Trypanosoma brucei EATRO1125.T7T and 427 90-13 | AMP + phosphoenolpyruvate + diphosphate | - |
r | |
2.7.9.1 | ATP + pyruvate + phosphate | PPDK functions in the glycolytic direction with production of ATP in the glycosomes | Trypanosoma brucei EATRO1125.T7T and 427 90-13 | AMP + phosphoenolpyruvate + diphosphate | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.9.1 | PPDK | - |
Trypanosoma brucei |
2.7.9.1 | pyruvate phosphate dikinase | - |
Trypanosoma brucei |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.9.1 | AMP | - |
Trypanosoma brucei | |
2.7.9.1 | ATP | - |
Trypanosoma brucei |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.9.1 | malfunction | deletion of the trypanosomal PPDK gene affects glycolysis. The rate of acetate production from glucose is 30% reduced in the DELTAppdk mutant, whereas threonine-derived acetate production is not affected. The DELTAppdk/DELTApepck double mutant, also lacking phosphoenolpyruvate carboxykinase, PEPCK, is incubated in glucose as the only carbon source and shows a 3.8fold reduction of the glycolytic rate compared with the DELTApepck mutant, as a consequence of the imbalanced glycosomal ATP/ADP ratio. Expressing the glycosomal phosphoglycerate kinase (PGKC) in the DELTAppdk/DELTApepck cell line restores the glycolytic flux, but expression of PGKC is lethal for procyclic trypanosomes, as a consequence of ATP depletion, due to glycosomal relocation of cytosolic ATP production. Comparison of glucose metabolism regulation of wild-type and mutant enzymes, the glucose metabolism is strongly impaired in the DELTAppdk/DELTApepck mutant, overview | Trypanosoma brucei |
2.7.9.1 | physiological function | pyruvate phosphate dikinase (PPDK) is a diphosphate-dependent enzyme, which reversibly catalyzes conversion of phosphoenolpyruvate, diphosphate, and AMP into pyruvate, phosphate, and ATP. In trypanosomes, enzyme PPDK works in the glycolytic direction and participates in the maintenance of the glycosomal ATP/ADP balance. The glycosomal PPDK provides a metabolic flexibility by producing 2 ATP per phosphoenolpyruvate consumed. Role of enzyme PPDK in acetate production | Trypanosoma brucei |