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Literature summary extracted from

  • Nomura, Y.; Izumi, A.; Fukunaga, Y.; Kusumi, K.; Iba, K.; Watanabe, S.; Nakahira, Y.; Weber, A.P.; Nozawa, A.; Tozawa, Y.
    Diversity in guanosine 3,5-bisdiphosphate (ppGpp) sensitivity among guanylate kinases of bacteria and plants (2014), J. Biol. Chem., 289, 15631-15641.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.8 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Bacillus subtilis
2.7.4.8 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Escherichia coli
2.7.4.8 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Synechococcus elongatus
2.7.4.8 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Saccharomyces cerevisiae
2.7.4.8 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Pisum sativum
2.7.4.8 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Oryza sativa Japonica Group
2.7.4.8 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.8 guanosine 3',5'-bisdiphosphate specific inhibition of the organellar isozyme Arabidopsis thaliana
2.7.4.8 guanosine 3',5'-bisdiphosphate specific inhibition of the isozyme Bacillus subtilis
2.7.4.8 guanosine 3',5'-bisdiphosphate specific inhibition of the organellar isozyme Oryza sativa Japonica Group
2.7.4.8 guanosine 3',5'-bisdiphosphate specific inhibition of the organellar isozyme Pisum sativum
2.7.4.8 additional information no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate Escherichia coli
2.7.4.8 additional information no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate Oryza sativa Japonica Group
2.7.4.8 additional information no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate Pisum sativum
2.7.4.8 additional information no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate Saccharomyces cerevisiae
2.7.4.8 additional information no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate Synechococcus elongatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.8 0.073
-
GMP recombinant chloroplast isozyme, pH 7.5, 30°C Oryza sativa Japonica Group
2.7.4.8 0.156
-
GMP recombinant cytosolic isozyme, pH 7.5, 30°C Escherichia coli
2.7.4.8 0.165
-
GMP recombinant isozyme, pH 7.5, 30°C Bacillus subtilis
2.7.4.8 0.202
-
GMP recombinant cytosolic isozyme, pH 7.5, 30°C Oryza sativa Japonica Group

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.8 chloroplast
-
Oryza sativa Japonica Group 9507
-
2.7.4.8 chloroplast
-
Arabidopsis thaliana 9507
-
2.7.4.8 chloroplast
-
Pisum sativum 9507
-
2.7.4.8 cytosol
-
Escherichia coli 5829
-
2.7.4.8 cytosol
-
Synechococcus elongatus 5829
-
2.7.4.8 cytosol
-
Saccharomyces cerevisiae 5829
-
2.7.4.8 cytosol
-
Pisum sativum 5829
-
2.7.4.8 cytosol
-
Oryza sativa Japonica Group 5829
-
2.7.4.8 additional information
-
Bacillus subtilis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.8 Mg2+ required Bacillus subtilis
2.7.4.8 Mg2+ required Escherichia coli
2.7.4.8 Mg2+ required Synechococcus elongatus
2.7.4.8 Mg2+ required Saccharomyces cerevisiae
2.7.4.8 Mg2+ required Pisum sativum
2.7.4.8 Mg2+ required Oryza sativa Japonica Group
2.7.4.8 Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.8 ATP + GMP Bacillus subtilis
-
ADP + GDP
-
?
2.7.4.8 ATP + GMP Escherichia coli
-
ADP + GDP
-
?
2.7.4.8 ATP + GMP Synechococcus elongatus
-
ADP + GDP
-
?
2.7.4.8 ATP + GMP Saccharomyces cerevisiae
-
ADP + GDP
-
?
2.7.4.8 ATP + GMP Pisum sativum
-
ADP + GDP
-
?
2.7.4.8 ATP + GMP Oryza sativa Japonica Group
-
ADP + GDP
-
?
2.7.4.8 ATP + GMP Arabidopsis thaliana
-
ADP + GDP
-
?
2.7.4.8 ATP + GMP Oryza sativa Japonica Group Nipponbare
-
ADP + GDP
-
?
2.7.4.8 ATP + GMP Synechococcus elongatus PCC 7942
-
ADP + GDP
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.8 Arabidopsis thaliana Q94JM2
-
-
2.7.4.8 Bacillus subtilis
-
-
-
2.7.4.8 Escherichia coli
-
-
-
2.7.4.8 Oryza sativa Japonica Group Q10M74
-
-
2.7.4.8 Oryza sativa Japonica Group Q2QPW1
-
-
2.7.4.8 Oryza sativa Japonica Group Nipponbare Q10M74
-
-
2.7.4.8 Oryza sativa Japonica Group Nipponbare Q2QPW1
-
-
2.7.4.8 Pisum sativum W8VNI6 cv. Toyonari, gene PsGKpm
-
2.7.4.8 Pisum sativum W8VZ39 cv. Toyonari, gene PsGKc
-
2.7.4.8 Saccharomyces cerevisiae P15454
-
-
2.7.4.8 Synechococcus elongatus
-
-
-
2.7.4.8 Synechococcus elongatus PCC 7942
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.8 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography Bacillus subtilis
2.7.4.8 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography Escherichia coli
2.7.4.8 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography Synechococcus elongatus
2.7.4.8 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography Saccharomyces cerevisiae
2.7.4.8 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography Pisum sativum
2.7.4.8 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography Oryza sativa Japonica Group
2.7.4.8 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography Arabidopsis thaliana
2.7.4.8 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography, native enzyme from chloroplasts by gel filtration Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.8 leaf
-
Pisum sativum
-
2.7.4.8 leaf
-
Oryza sativa Japonica Group
-
2.7.4.8 leaf
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.8 ATP + GMP
-
Bacillus subtilis ADP + GDP
-
?
2.7.4.8 ATP + GMP
-
Escherichia coli ADP + GDP
-
?
2.7.4.8 ATP + GMP
-
Synechococcus elongatus ADP + GDP
-
?
2.7.4.8 ATP + GMP
-
Saccharomyces cerevisiae ADP + GDP
-
?
2.7.4.8 ATP + GMP
-
Pisum sativum ADP + GDP
-
?
2.7.4.8 ATP + GMP
-
Oryza sativa Japonica Group ADP + GDP
-
?
2.7.4.8 ATP + GMP
-
Arabidopsis thaliana ADP + GDP
-
?
2.7.4.8 ATP + GMP
-
Oryza sativa Japonica Group Nipponbare ADP + GDP
-
?
2.7.4.8 ATP + GMP
-
Synechococcus elongatus PCC 7942 ADP + GDP
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.8 GK3
-
Arabidopsis thaliana
2.7.4.8 GKc
-
Pisum sativum
2.7.4.8 GKpm
-
Oryza sativa Japonica Group
2.7.4.8 GKpm
-
Arabidopsis thaliana
2.7.4.8 GKpm
-
Pisum sativum
2.7.4.8 GMK3
-
Arabidopsis thaliana
2.7.4.8 organellar GK
-
Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.8 30
-
assay at Bacillus subtilis
2.7.4.8 30
-
assay at Escherichia coli
2.7.4.8 30
-
assay at Synechococcus elongatus
2.7.4.8 30
-
assay at Saccharomyces cerevisiae
2.7.4.8 30
-
assay at Pisum sativum
2.7.4.8 30
-
assay at Oryza sativa Japonica Group
2.7.4.8 30
-
assay at Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.8 23
-
GMP recombinant chloroplast isozyme, pH 7.5, 30°C Oryza sativa Japonica Group
2.7.4.8 52
-
GMP recombinant isozyme, pH 7.5, 30°C Bacillus subtilis
2.7.4.8 70
-
GMP recombinant cytosolic isozyme, pH 7.5, 30°C Oryza sativa Japonica Group
2.7.4.8 209
-
GMP recombinant cytosolic isozyme, pH 7.5, 30°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.8 7.5
-
assay at Bacillus subtilis
2.7.4.8 7.5
-
assay at Escherichia coli
2.7.4.8 7.5
-
assay at Synechococcus elongatus
2.7.4.8 7.5
-
assay at Saccharomyces cerevisiae
2.7.4.8 7.5
-
assay at Pisum sativum
2.7.4.8 7.5
-
assay at Oryza sativa Japonica Group
2.7.4.8 7.5
-
assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.8 ATP
-
Bacillus subtilis
2.7.4.8 ATP
-
Escherichia coli
2.7.4.8 ATP
-
Synechococcus elongatus
2.7.4.8 ATP
-
Saccharomyces cerevisiae
2.7.4.8 ATP
-
Pisum sativum
2.7.4.8 ATP
-
Oryza sativa Japonica Group
2.7.4.8 ATP
-
Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.8 0.0028
-
guanosine 3',5'-bisdiphosphate versus GMP, pH 7.5, 30°C Oryza sativa Japonica Group

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.4.8 0.01
-
versus GMP, pH 7.5, 30°C Oryza sativa Japonica Group guanosine 3',5'-bisdiphosphate

General Information

EC Number General Information Comment Organism
2.7.4.8 metabolism guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview Bacillus subtilis
2.7.4.8 metabolism guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview Synechococcus elongatus
2.7.4.8 metabolism guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview Saccharomyces cerevisiae
2.7.4.8 metabolism guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview Pisum sativum
2.7.4.8 metabolism guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview Oryza sativa Japonica Group
2.7.4.8 metabolism guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview Arabidopsis thaliana
2.7.4.8 metabolism guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview. Accumulation of guanosine 3',5'-bisdiphosphate has little effect on the guanine nucleotide profile of Escherichia coli Escherichia coli
2.7.4.8 physiological function guanylate kinase is a key enzyme in guanine nucleotide biosynthesis that catalyzes the conversion of GMP to GDP, is a target of regulation by guanosine 3',5'-bisdiphosphate in chloroplasts. Plants have two distinct types of GK that are localized to organelles (GKpm) or to the cytosol (GKc) and both are essential for growth and development Pisum sativum
2.7.4.8 physiological function guanylate kinase is a key enzyme in guanine nucleotide biosynthesis that catalyzes the conversion of GMP to GDP, is a target of regulation by guanosine 3',5'-bisdiphosphate in chloroplasts. Plants have two distinct types of GK that are localized to organelles (GKpm) or to the cytosol (GKc) and both are essential for growth and development. guanosine 3',5'-bisdiphosphate plays a pivotal role in the regulation of GTP biosynthesis in chloroplasts through specific inhibition of GKpm activity, with the regulation of GTP biosynthesis in chloroplasts thus being independent of that in the cytosol Pisum sativum
2.7.4.8 physiological function guanylate kinase is a key enzyme in guanine nucleotide biosynthesis that catalyzes the conversion of GMP to GDP, is a target of regulation by guanosine 3',5'-bisdiphosphate in chloroplasts. Plants have two distinct types of GK that are localized to organelles (GKpm) or to the cytosol (GKc) and both are essential for growth and development. guanosine 3',5'-bisdiphosphate plays a pivotal role in the regulation of GTP biosynthesis in chloroplasts through specific inhibition ofGKpmactivity, with the regulation of GTP biosynthesis in chloroplasts thus being independent of that in the cytosol Oryza sativa Japonica Group
2.7.4.8 physiological function guanylate kinase is a key enzyme in guanine nucleotide biosynthesis that catalyzes the conversion of GMP to GDP, is a target of regulation by guanosine 3',5'-bisdiphosphate in chloroplasts. Plants have two distinct types of GK that are localized to organelles (GKpm) or to the cytosol (GKc) and both are essential for growth and development. guanosine 3',5'-bisdiphosphate plays a pivotal role in the regulation of GTP biosynthesis in chloroplasts through specific inhibition ofGKpmactivity, with the regulation of GTP biosynthesis in chloroplasts thus being independent of that in the cytosol Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.4.8 314
-
GMP recombinant isozyme, pH 7.5, 30°C Bacillus subtilis
2.7.4.8 317
-
GMP recombinant chloroplast isozyme, pH 7.5, 30°C Oryza sativa Japonica Group
2.7.4.8 344
-
GMP recombinant cytosolic isozyme, pH 7.5, 30°C Oryza sativa Japonica Group
2.7.4.8 1347
-
GMP recombinant cytosolic isozyme, pH 7.5, 30°C Escherichia coli