EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.4.8 | recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
2.7.4.8 | recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
2.7.4.8 | recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Synechococcus elongatus |
2.7.4.8 | recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
2.7.4.8 | recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Pisum sativum |
2.7.4.8 | recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Oryza sativa Japonica Group |
2.7.4.8 | recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.8 | guanosine 3',5'-bisdiphosphate | specific inhibition of the organellar isozyme | Arabidopsis thaliana | |
2.7.4.8 | guanosine 3',5'-bisdiphosphate | specific inhibition of the isozyme | Bacillus subtilis | |
2.7.4.8 | guanosine 3',5'-bisdiphosphate | specific inhibition of the organellar isozyme | Oryza sativa Japonica Group | |
2.7.4.8 | guanosine 3',5'-bisdiphosphate | specific inhibition of the organellar isozyme | Pisum sativum | |
2.7.4.8 | additional information | no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate | Escherichia coli | |
2.7.4.8 | additional information | no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate | Oryza sativa Japonica Group | |
2.7.4.8 | additional information | no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate | Pisum sativum | |
2.7.4.8 | additional information | no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate | Saccharomyces cerevisiae | |
2.7.4.8 | additional information | no inhibition of the cytosolic isozyme by guanosine 3',5'-bisdiphosphate | Synechococcus elongatus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.8 | 0.073 | - |
GMP | recombinant chloroplast isozyme, pH 7.5, 30°C | Oryza sativa Japonica Group | |
2.7.4.8 | 0.156 | - |
GMP | recombinant cytosolic isozyme, pH 7.5, 30°C | Escherichia coli | |
2.7.4.8 | 0.165 | - |
GMP | recombinant isozyme, pH 7.5, 30°C | Bacillus subtilis | |
2.7.4.8 | 0.202 | - |
GMP | recombinant cytosolic isozyme, pH 7.5, 30°C | Oryza sativa Japonica Group |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.4.8 | chloroplast | - |
Oryza sativa Japonica Group | 9507 | - |
2.7.4.8 | chloroplast | - |
Arabidopsis thaliana | 9507 | - |
2.7.4.8 | chloroplast | - |
Pisum sativum | 9507 | - |
2.7.4.8 | cytosol | - |
Escherichia coli | 5829 | - |
2.7.4.8 | cytosol | - |
Synechococcus elongatus | 5829 | - |
2.7.4.8 | cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
2.7.4.8 | cytosol | - |
Pisum sativum | 5829 | - |
2.7.4.8 | cytosol | - |
Oryza sativa Japonica Group | 5829 | - |
2.7.4.8 | additional information | - |
Bacillus subtilis | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.8 | Mg2+ | required | Bacillus subtilis | |
2.7.4.8 | Mg2+ | required | Escherichia coli | |
2.7.4.8 | Mg2+ | required | Synechococcus elongatus | |
2.7.4.8 | Mg2+ | required | Saccharomyces cerevisiae | |
2.7.4.8 | Mg2+ | required | Pisum sativum | |
2.7.4.8 | Mg2+ | required | Oryza sativa Japonica Group | |
2.7.4.8 | Mg2+ | required | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.8 | ATP + GMP | Bacillus subtilis | - |
ADP + GDP | - |
? | |
2.7.4.8 | ATP + GMP | Escherichia coli | - |
ADP + GDP | - |
? | |
2.7.4.8 | ATP + GMP | Synechococcus elongatus | - |
ADP + GDP | - |
? | |
2.7.4.8 | ATP + GMP | Saccharomyces cerevisiae | - |
ADP + GDP | - |
? | |
2.7.4.8 | ATP + GMP | Pisum sativum | - |
ADP + GDP | - |
? | |
2.7.4.8 | ATP + GMP | Oryza sativa Japonica Group | - |
ADP + GDP | - |
? | |
2.7.4.8 | ATP + GMP | Arabidopsis thaliana | - |
ADP + GDP | - |
? | |
2.7.4.8 | ATP + GMP | Oryza sativa Japonica Group Nipponbare | - |
ADP + GDP | - |
? | |
2.7.4.8 | ATP + GMP | Synechococcus elongatus PCC 7942 | - |
ADP + GDP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.8 | Arabidopsis thaliana | Q94JM2 | - |
- |
2.7.4.8 | Bacillus subtilis | - |
- |
- |
2.7.4.8 | Escherichia coli | - |
- |
- |
2.7.4.8 | Oryza sativa Japonica Group | Q10M74 | - |
- |
2.7.4.8 | Oryza sativa Japonica Group | Q2QPW1 | - |
- |
2.7.4.8 | Oryza sativa Japonica Group Nipponbare | Q10M74 | - |
- |
2.7.4.8 | Oryza sativa Japonica Group Nipponbare | Q2QPW1 | - |
- |
2.7.4.8 | Pisum sativum | W8VNI6 | cv. Toyonari, gene PsGKpm | - |
2.7.4.8 | Pisum sativum | W8VZ39 | cv. Toyonari, gene PsGKc | - |
2.7.4.8 | Saccharomyces cerevisiae | P15454 | - |
- |
2.7.4.8 | Synechococcus elongatus | - |
- |
- |
2.7.4.8 | Synechococcus elongatus PCC 7942 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.4.8 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography | Bacillus subtilis |
2.7.4.8 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography | Escherichia coli |
2.7.4.8 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography | Synechococcus elongatus |
2.7.4.8 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography | Saccharomyces cerevisiae |
2.7.4.8 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography | Pisum sativum |
2.7.4.8 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography | Oryza sativa Japonica Group |
2.7.4.8 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography | Arabidopsis thaliana |
2.7.4.8 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography, native enzyme from chloroplasts by gel filtration | Pisum sativum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.4.8 | leaf | - |
Pisum sativum | - |
2.7.4.8 | leaf | - |
Oryza sativa Japonica Group | - |
2.7.4.8 | leaf | - |
Arabidopsis thaliana | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.8 | ATP + GMP | - |
Bacillus subtilis | ADP + GDP | - |
? | |
2.7.4.8 | ATP + GMP | - |
Escherichia coli | ADP + GDP | - |
? | |
2.7.4.8 | ATP + GMP | - |
Synechococcus elongatus | ADP + GDP | - |
? | |
2.7.4.8 | ATP + GMP | - |
Saccharomyces cerevisiae | ADP + GDP | - |
? | |
2.7.4.8 | ATP + GMP | - |
Pisum sativum | ADP + GDP | - |
? | |
2.7.4.8 | ATP + GMP | - |
Oryza sativa Japonica Group | ADP + GDP | - |
? | |
2.7.4.8 | ATP + GMP | - |
Arabidopsis thaliana | ADP + GDP | - |
? | |
2.7.4.8 | ATP + GMP | - |
Oryza sativa Japonica Group Nipponbare | ADP + GDP | - |
? | |
2.7.4.8 | ATP + GMP | - |
Synechococcus elongatus PCC 7942 | ADP + GDP | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.4.8 | GK3 | - |
Arabidopsis thaliana |
2.7.4.8 | GKc | - |
Pisum sativum |
2.7.4.8 | GKpm | - |
Oryza sativa Japonica Group |
2.7.4.8 | GKpm | - |
Arabidopsis thaliana |
2.7.4.8 | GKpm | - |
Pisum sativum |
2.7.4.8 | GMK3 | - |
Arabidopsis thaliana |
2.7.4.8 | organellar GK | - |
Bacillus subtilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.4.8 | 30 | - |
assay at | Bacillus subtilis |
2.7.4.8 | 30 | - |
assay at | Escherichia coli |
2.7.4.8 | 30 | - |
assay at | Synechococcus elongatus |
2.7.4.8 | 30 | - |
assay at | Saccharomyces cerevisiae |
2.7.4.8 | 30 | - |
assay at | Pisum sativum |
2.7.4.8 | 30 | - |
assay at | Oryza sativa Japonica Group |
2.7.4.8 | 30 | - |
assay at | Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.8 | 23 | - |
GMP | recombinant chloroplast isozyme, pH 7.5, 30°C | Oryza sativa Japonica Group | |
2.7.4.8 | 52 | - |
GMP | recombinant isozyme, pH 7.5, 30°C | Bacillus subtilis | |
2.7.4.8 | 70 | - |
GMP | recombinant cytosolic isozyme, pH 7.5, 30°C | Oryza sativa Japonica Group | |
2.7.4.8 | 209 | - |
GMP | recombinant cytosolic isozyme, pH 7.5, 30°C | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.4.8 | 7.5 | - |
assay at | Bacillus subtilis |
2.7.4.8 | 7.5 | - |
assay at | Escherichia coli |
2.7.4.8 | 7.5 | - |
assay at | Synechococcus elongatus |
2.7.4.8 | 7.5 | - |
assay at | Saccharomyces cerevisiae |
2.7.4.8 | 7.5 | - |
assay at | Pisum sativum |
2.7.4.8 | 7.5 | - |
assay at | Oryza sativa Japonica Group |
2.7.4.8 | 7.5 | - |
assay at | Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.8 | ATP | - |
Bacillus subtilis | |
2.7.4.8 | ATP | - |
Escherichia coli | |
2.7.4.8 | ATP | - |
Synechococcus elongatus | |
2.7.4.8 | ATP | - |
Saccharomyces cerevisiae | |
2.7.4.8 | ATP | - |
Pisum sativum | |
2.7.4.8 | ATP | - |
Oryza sativa Japonica Group | |
2.7.4.8 | ATP | - |
Arabidopsis thaliana |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.8 | 0.0028 | - |
guanosine 3',5'-bisdiphosphate | versus GMP, pH 7.5, 30°C | Oryza sativa Japonica Group |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.7.4.8 | 0.01 | - |
versus GMP, pH 7.5, 30°C | Oryza sativa Japonica Group | guanosine 3',5'-bisdiphosphate |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.4.8 | metabolism | guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview | Bacillus subtilis |
2.7.4.8 | metabolism | guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview | Synechococcus elongatus |
2.7.4.8 | metabolism | guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview | Saccharomyces cerevisiae |
2.7.4.8 | metabolism | guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview | Pisum sativum |
2.7.4.8 | metabolism | guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview | Oryza sativa Japonica Group |
2.7.4.8 | metabolism | guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview | Arabidopsis thaliana |
2.7.4.8 | metabolism | guanylate kinase is a key enzyme in guanine nucleotide biosynthesis, purine biosynthetic pathways in plant cells and bacteria, overview. Accumulation of guanosine 3',5'-bisdiphosphate has little effect on the guanine nucleotide profile of Escherichia coli | Escherichia coli |
2.7.4.8 | physiological function | guanylate kinase is a key enzyme in guanine nucleotide biosynthesis that catalyzes the conversion of GMP to GDP, is a target of regulation by guanosine 3',5'-bisdiphosphate in chloroplasts. Plants have two distinct types of GK that are localized to organelles (GKpm) or to the cytosol (GKc) and both are essential for growth and development | Pisum sativum |
2.7.4.8 | physiological function | guanylate kinase is a key enzyme in guanine nucleotide biosynthesis that catalyzes the conversion of GMP to GDP, is a target of regulation by guanosine 3',5'-bisdiphosphate in chloroplasts. Plants have two distinct types of GK that are localized to organelles (GKpm) or to the cytosol (GKc) and both are essential for growth and development. guanosine 3',5'-bisdiphosphate plays a pivotal role in the regulation of GTP biosynthesis in chloroplasts through specific inhibition of GKpm activity, with the regulation of GTP biosynthesis in chloroplasts thus being independent of that in the cytosol | Pisum sativum |
2.7.4.8 | physiological function | guanylate kinase is a key enzyme in guanine nucleotide biosynthesis that catalyzes the conversion of GMP to GDP, is a target of regulation by guanosine 3',5'-bisdiphosphate in chloroplasts. Plants have two distinct types of GK that are localized to organelles (GKpm) or to the cytosol (GKc) and both are essential for growth and development. guanosine 3',5'-bisdiphosphate plays a pivotal role in the regulation of GTP biosynthesis in chloroplasts through specific inhibition ofGKpmactivity, with the regulation of GTP biosynthesis in chloroplasts thus being independent of that in the cytosol | Oryza sativa Japonica Group |
2.7.4.8 | physiological function | guanylate kinase is a key enzyme in guanine nucleotide biosynthesis that catalyzes the conversion of GMP to GDP, is a target of regulation by guanosine 3',5'-bisdiphosphate in chloroplasts. Plants have two distinct types of GK that are localized to organelles (GKpm) or to the cytosol (GKc) and both are essential for growth and development. guanosine 3',5'-bisdiphosphate plays a pivotal role in the regulation of GTP biosynthesis in chloroplasts through specific inhibition ofGKpmactivity, with the regulation of GTP biosynthesis in chloroplasts thus being independent of that in the cytosol | Arabidopsis thaliana |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.8 | 314 | - |
GMP | recombinant isozyme, pH 7.5, 30°C | Bacillus subtilis | |
2.7.4.8 | 317 | - |
GMP | recombinant chloroplast isozyme, pH 7.5, 30°C | Oryza sativa Japonica Group | |
2.7.4.8 | 344 | - |
GMP | recombinant cytosolic isozyme, pH 7.5, 30°C | Oryza sativa Japonica Group | |
2.7.4.8 | 1347 | - |
GMP | recombinant cytosolic isozyme, pH 7.5, 30°C | Escherichia coli |