EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.1 | gene Pof1 or YCL047C, DNA and amino acid sequence determination and analysis, sequence comparisons, construction of the POF1-URA3 episomal plasmid (pRS316-POF1) derived from pADH1-POF1, which is constructed in the integrative pPP81 (LEU2) vector, recombinant expression of His-tagged enzyme from plasmid pET28-POF1 in Escherichia coli strain Rosetta (DE3), quantitative PCR expression analysis | Saccharomyces cerevisiae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.1 | additional information | construction of a haploid single gene deletion mutant, cells lacking the ORF YCL047C/POF1 display strong cross-feeding activity | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.1 | 2.26 | - |
nicotinamide ribonucleotide | pH 10.0, 30°C, recombinant enzyme | Saccharomyces cerevisiae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.1 | Mg2+ | required | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.1 | ATP + nicotinamide ribonucleotide | Saccharomyces cerevisiae | - |
diphosphate + NAD+ | - |
? | |
2.7.7.1 | ATP + nicotinamide ribonucleotide | Saccharomyces cerevisiae BY4742 | - |
diphosphate + NAD+ | - |
? | |
2.7.7.1 | additional information | Saccharomyces cerevisiae | the amino acid sequence of Pof1 indicates that it is a putative nicotinamide mononucleotide adenylyltransferase (NMNAT). Unlike other yeast NMNATs, Pof1 exhibits NMN-specific adenylyltransferase activity | ? | - |
? | |
2.7.7.1 | additional information | Saccharomyces cerevisiae BY4742 | the amino acid sequence of Pof1 indicates that it is a putative nicotinamide mononucleotide adenylyltransferase (NMNAT). Unlike other yeast NMNATs, Pof1 exhibits NMN-specific adenylyltransferase activity | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.1 | Saccharomyces cerevisiae | P25576 | gene Pof1 or YCL047C | - |
2.7.7.1 | Saccharomyces cerevisiae BY4742 | P25576 | gene Pof1 or YCL047C | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.1 | ATP + nicotinamide ribonucleotide | - |
Saccharomyces cerevisiae | diphosphate + NAD+ | - |
? | |
2.7.7.1 | ATP + nicotinamide ribonucleotide | - |
Saccharomyces cerevisiae BY4742 | diphosphate + NAD+ | - |
? | |
2.7.7.1 | additional information | the amino acid sequence of Pof1 indicates that it is a putative nicotinamide mononucleotide adenylyltransferase (NMNAT). Unlike other yeast NMNATs, Pof1 exhibits NMN-specific adenylyltransferase activity | Saccharomyces cerevisiae | ? | - |
? | |
2.7.7.1 | additional information | the amino acid sequence of Pof1 indicates that it is a putative nicotinamide mononucleotide adenylyltransferase (NMNAT). Unlike other yeast NMNATs, Pof1 exhibits NMN-specific adenylyltransferase activity | Saccharomyces cerevisiae BY4742 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.1 | mononucleotide adenylyltransferase | - |
Saccharomyces cerevisiae |
2.7.7.1 | NMNAT | - |
Saccharomyces cerevisiae |
2.7.7.1 | Pof1 | - |
Saccharomyces cerevisiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.1 | 30 | - |
assay at | Saccharomyces cerevisiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.1 | 35.33 | - |
nicotinamide ribonucleotide | pH 10.0, 30°C, recombinant enzyme | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.1 | 10 | - |
assay at | Saccharomyces cerevisiae |
EC Number | Organism | Comment | Expression |
---|---|---|---|
2.7.7.1 | Saccharomyces cerevisiae | expression of POF1 is slightly induced in the late log phase | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.7.1 | malfunction | deletion of POF1 significantly lowers NAD+ levels and decreases the efficiency of nicotinamide riboside utilization, resistance to oxidative stress, and nicotinamide riboside-induced life span extension | Saccharomyces cerevisiae |