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Literature summary extracted from

  • Kato, M.; Lin, S.J.
    YCL047C/POF1 is a novel nicotinamide mononucleotide adenylyltransferase (NMNAT) in Saccharomyces cerevisiae (2014), J. Biol. Chem., 289, 15577-15587.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.1 gene Pof1 or YCL047C, DNA and amino acid sequence determination and analysis, sequence comparisons, construction of the POF1-URA3 episomal plasmid (pRS316-POF1) derived from pADH1-POF1, which is constructed in the integrative pPP81 (LEU2) vector, recombinant expression of His-tagged enzyme from plasmid pET28-POF1 in Escherichia coli strain Rosetta (DE3), quantitative PCR expression analysis Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.1 additional information construction of a haploid single gene deletion mutant, cells lacking the ORF YCL047C/POF1 display strong cross-feeding activity Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.1 2.26
-
nicotinamide ribonucleotide pH 10.0, 30°C, recombinant enzyme Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.1 Mg2+ required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide Saccharomyces cerevisiae
-
diphosphate + NAD+
-
?
2.7.7.1 ATP + nicotinamide ribonucleotide Saccharomyces cerevisiae BY4742
-
diphosphate + NAD+
-
?
2.7.7.1 additional information Saccharomyces cerevisiae the amino acid sequence of Pof1 indicates that it is a putative nicotinamide mononucleotide adenylyltransferase (NMNAT). Unlike other yeast NMNATs, Pof1 exhibits NMN-specific adenylyltransferase activity ?
-
?
2.7.7.1 additional information Saccharomyces cerevisiae BY4742 the amino acid sequence of Pof1 indicates that it is a putative nicotinamide mononucleotide adenylyltransferase (NMNAT). Unlike other yeast NMNATs, Pof1 exhibits NMN-specific adenylyltransferase activity ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.1 Saccharomyces cerevisiae P25576 gene Pof1 or YCL047C
-
2.7.7.1 Saccharomyces cerevisiae BY4742 P25576 gene Pof1 or YCL047C
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide
-
Saccharomyces cerevisiae diphosphate + NAD+
-
?
2.7.7.1 ATP + nicotinamide ribonucleotide
-
Saccharomyces cerevisiae BY4742 diphosphate + NAD+
-
?
2.7.7.1 additional information the amino acid sequence of Pof1 indicates that it is a putative nicotinamide mononucleotide adenylyltransferase (NMNAT). Unlike other yeast NMNATs, Pof1 exhibits NMN-specific adenylyltransferase activity Saccharomyces cerevisiae ?
-
?
2.7.7.1 additional information the amino acid sequence of Pof1 indicates that it is a putative nicotinamide mononucleotide adenylyltransferase (NMNAT). Unlike other yeast NMNATs, Pof1 exhibits NMN-specific adenylyltransferase activity Saccharomyces cerevisiae BY4742 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.1 mononucleotide adenylyltransferase
-
Saccharomyces cerevisiae
2.7.7.1 NMNAT
-
Saccharomyces cerevisiae
2.7.7.1 Pof1
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.1 30
-
assay at Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.1 35.33
-
nicotinamide ribonucleotide pH 10.0, 30°C, recombinant enzyme Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.1 10
-
assay at Saccharomyces cerevisiae

Expression

EC Number Organism Comment Expression
2.7.7.1 Saccharomyces cerevisiae expression of POF1 is slightly induced in the late log phase up

General Information

EC Number General Information Comment Organism
2.7.7.1 malfunction deletion of POF1 significantly lowers NAD+ levels and decreases the efficiency of nicotinamide riboside utilization, resistance to oxidative stress, and nicotinamide riboside-induced life span extension Saccharomyces cerevisiae