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Literature summary extracted from

  • Ozaki, T.; Nishiyama, M.; Kuzuyama, T.
    Novel tryptophan metabolism by a potential gene cluster that is widely distributed among Actinomycetes (2013), J. Biol. Chem., 288, 9946-9956.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.B31 gene SCO7468, genetic organization and sequence comparison, functional recombinant expression in Streptomyces lividans strain TK23, the overexpression of 5-DMATSSc results in 5-dimethylallylindole-3-acetonitrile overproduction in the transformant. The heterologous host Streptomyces lividans TK23 possesses a gene cluster almost identical to that of Streptomyces coelicolor A3(2). Recombinant expression in Escherichia coli strain BL21(DE3) Streptomyces coelicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.B31 additional information no inhibition by EDTA at 5 mM Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.B31 additional information
-
 additional information steady-state kinetic analysis demonstrates that the SCO7467 reaction follows Michaelis-Menten kinetics Streptomyces coelicolor
2.5.1.B31 0.05
-
 dimethylallyl diphosphate pH 8.0, 30°C, recombinant enzyme Streptomyces coelicolor
2.5.1.B31 0.08
-
 L-tryptophan pH 8.0, 30°C, recombinant enzyme Streptomyces coelicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.B31 additional information the SCO7467-catalyzed prenylation reaction is Mg2+-independent Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.B31 dimethylallyl diphosphate + L-tryptophan Streptomyces coelicolor
-
 diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.B31 dimethylallyl diphosphate + L-tryptophan Streptomyces coelicolor A3(2)
-
 diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.B31 Streptomyces coelicolor Q9ADJ2 gene SCO7467
-
2.5.1.B31 Streptomyces coelicolor A3(2) Q9ADJ2 gene SCO7467
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.B31 recombinant enzyme from in Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.B31 dimethylallyl diphosphate + L-tryptophan
-
 Streptomyces coelicolor diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.B31 dimethylallyl diphosphate + L-tryptophan
-
 Streptomyces coelicolor diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan product identification by NMR and mass spectroscopy ?
2.5.1.B31 dimethylallyl diphosphate + L-tryptophan
-
 Streptomyces coelicolor A3(2) diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.B31 dimethylallyl diphosphate + L-tryptophan
-
 Streptomyces coelicolor A3(2) diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan product identification by NMR and mass spectroscopy ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.B31 5-DMATSSc
-
 Streptomyces coelicolor
2.5.1.B31 SCO7467
-
 Streptomyces coelicolor
2.5.1.B31 tryptophan C5-prenyltransferase
-
 Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.B31 30
-
 assay at Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.B31 0.4
-
 L-tryptophan pH 8.0, 30°C, recombinant enzyme Streptomyces coelicolor
2.5.1.B31 0.4
-
 dimethylallyl diphosphate pH 8.0, 30°C, recombinant enzyme Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.B31 8
-
 assay at Streptomyces coelicolor