Literature summary extracted from

Meng, L.; Forouhar, F.; Thieker, D.; Gao, Z.; Ramiah, A.; Moniz, H.; Xiang, Y.; Seetharaman, J.; Milaninia, S.; Su, M.; Bridger, R.; Veillon, L.; Azadi, P.; Kornhaber, G.; Wells, L.; Montelione, G.T.; Woods, R.J.; Tong, L.; Moremen, K.W.
Enzymatic basis for N-glycan sialylation: structure of rat alpha2,6-sialyltransferase (ST6GAL1) reveals conserved and unique features for glycan sialylation (2013), J. Biol. Chem., 288, 34680-34698.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.4.3.1	expressed in HEK-293 and 293-F cells	Rattus norvegicus

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.4.3.1	microbatch method, using 100 mM Tris, pH 9, 40% (w/v) PEG 1000, and 100 mM lithium bromide	Rattus norvegicus

Protein Variants

EC Number	Protein Variants	Comment	Organism
2.4.3.1	C350A/C361A	inactive	Rattus norvegicus
2.4.3.1	D271A	the mutant shows strongly reduced activity compared to the wild type enzyme	Rattus norvegicus
2.4.3.1	F208A	the mutant shows strongly reduced activity compared to the wild type enzyme	Rattus norvegicus
2.4.3.1	H367A	inactive	Rattus norvegicus
2.4.3.1	K355A	the mutant shows increased activity compared to the wild type enzyme	Rattus norvegicus
2.4.3.1	N230A	inactive	Rattus norvegicus
2.4.3.1	Q232A	the mutant shows strongly reduced activity compared to the wild type enzyme	Rattus norvegicus
2.4.3.1	Q354A	the mutant shows reduced activity compared to the wild type enzyme	Rattus norvegicus
2.4.3.1	Y119F	the mutant shows reduced activity compared to the wild type enzyme	Rattus norvegicus
2.4.3.1	Y272A	the mutant shows strongly reduced activity compared to the wild type enzyme	Rattus norvegicus
2.4.3.1	Y366A	inactive	Rattus norvegicus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.4.3.1	0.078	-	CMP-N-acetylneuraminate	mutant enzyme Y119F, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.092	-	CMP-N-acetylneuraminate	recombinant wild type enzyme from 293-F cells, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.093	-	CMP-N-acetylneuraminate	recombinant wild type enzyme from HEK-293 cells, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.095	-	CMP-N-acetylneuraminate	mutant enzyme K355A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.1	-	CMP-N-acetylneuraminate	mutant enzyme Q354A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.133	-	CMP-N-acetylneuraminate	mutant enzyme Q232A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.22	-	CMP-N-acetylneuraminate	mutant enzyme Y272A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.365	-	CMP-N-acetylneuraminate	mutant enzyme D271A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.4	-	CMP-N-acetylneuraminate	mutant enzyme F208A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	1	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme F208A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	1.1	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme K355A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	1.4	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	recombinant wild type enzyme from HEK-293 cells, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	1.8	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme Q354A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	1.8	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme Y119F, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	1.8	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	recombinant wild type enzyme from 293-F cells, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	2.6	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme Q232A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	3.5	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme Y272A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	6.5	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme D271A, at pH 6.5 and 37°C	Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.4.3.1	CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	Rattus norvegicus	-	CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.3.1	Rattus norvegicus	P13721	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.4.3.1	Ni-NTA column chromatography and Superdex-75 gel filtration	Rattus norvegicus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.4.3.1	CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	-	Rattus norvegicus	CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
2.4.3.1	alpha2,6-sialyltransferase	-	Rattus norvegicus
2.4.3.1	ST6Gal1	-	Rattus norvegicus

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.4.3.1	0.05	-	CMP-N-acetylneuraminate	mutant enzyme D271A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.05	-	CMP-N-acetylneuraminate	mutant enzyme Y272A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.07	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme Y272A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.09	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme D271A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.1	-	CMP-N-acetylneuraminate	mutant enzyme Q232A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.14	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme Q232A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.19	-	CMP-N-acetylneuraminate	mutant enzyme F208A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.31	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme Y119F, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.35	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	recombinant wild type enzyme from HEK-293 cells, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.39	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme K355A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.39	-	CMP-N-acetylneuraminate	mutant enzyme Y119F, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.42	-	CMP-N-acetylneuraminate	mutant enzyme Q354A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.43	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme F208A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.49	-	CMP-N-acetylneuraminate	recombinant wild type enzyme from HEK-293 cells, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.59	-	CMP-N-acetylneuraminate	recombinant wild type enzyme from 293-F cells, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.64	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme Q354A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.67	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	recombinant wild type enzyme from 293-F cells, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.69	-	CMP-N-acetylneuraminate	mutant enzyme K355A, at pH 6.5 and 37°C	Rattus norvegicus

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2.4.3.1	0.01	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme D271A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.02	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme Y272A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.05	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme Q232A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.13	-	CMP-N-acetylneuraminate	mutant enzyme D271A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.17	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme Y119F, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.23	-	CMP-N-acetylneuraminate	mutant enzyme Y272A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.25	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	recombinant wild type enzyme from HEK-293 cells, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.36	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme K355A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.36	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme Q354A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.37	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	recombinant wild type enzyme from 293-F cells, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.44	-	beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine	mutant enzyme F208A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	0.47	-	CMP-N-acetylneuraminate	mutant enzyme F208A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	4.17	-	CMP-N-acetylneuraminate	mutant enzyme Q354A, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	5	-	CMP-N-acetylneuraminate	mutant enzyme Y119F, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	5.27	-	CMP-N-acetylneuraminate	recombinant wild type enzyme from HEK-293 cells, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	6.45	-	CMP-N-acetylneuraminate	recombinant wild type enzyme from 293-F cells, at pH 6.5 and 37°C	Rattus norvegicus
2.4.3.1	7.3	-	CMP-N-acetylneuraminate	mutant enzyme K355A, at pH 6.5 and 37°C	Rattus norvegicus

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Release 2023.1 (January 2023)