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Literature summary extracted from

  • Qiu, Y.; Fakas, S.; Han, G.S.; Barbosa, A.D.; Siniossoglou, S.; Carman, G.M.
    Transcription factor Reb1p regulates DGK1-encoded diacylglycerol kinase and lipid metabolism in Saccharomyces cerevisiae (2013), J. Biol. Chem., 288, 29124-29133.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.174 endoplasmic reticulum associated with Saccharomyces cerevisiae 5783
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.174 Ca2+ required Saccharomyces cerevisiae
2.7.1.174 Mg2+ required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.174 CTP + 1,2-diacyl-sn-glycerol Saccharomyces cerevisiae
-
 CDP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.174 Saccharomyces cerevisiae Q12382 gene DGK1
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.174 CTP + 1,2-diacyl-sn-glycerol
-
 Saccharomyces cerevisiae CDP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.174 CTP-dependent diacylglycerol kinase
-
 Saccharomyces cerevisiae
2.7.1.174 DAG kinase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.174 30
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.174 7.5
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.174 CTP
-
 Saccharomyces cerevisiae

Expression

EC Number Organism Comment Expression
2.7.1.174 Saccharomyces cerevisiae expression of diacylglycerol kinase is induced by the Reb1p transcription factor, Reb1p directly interacts with the Reb1p-binding sequence in the DGK1 promoter up

General Information

EC Number General Information Comment Organism
2.7.1.174 malfunction a Reb1p-binding site mutation abolishes the DGK1-mediated nuclear/endoplasmic reticulum membrane expansion Saccharomyces cerevisiae
2.7.1.174 physiological function the enzyme catalyzes the CTP-dependent phosphorylation of diacylglycerol to form phosphatidate, and in conjunction with PAH1-encoded phosphatidate phosphatase, it controls the levels of phosphatidate and diacylglycerol for phospholipid synthesis, membrane growth, and lipid droplet formation. A functional level of diacylglycerol kinase is regulated by the Reb1p transcription factor. Reb1p specifically binds the consensus recognition sequence (CGGGTAA, -166 to -160) in the DGK1 promoter. Diacylglycerol kinase is regulated at the level of transcription. The expression of diacylglycerol kinase is induced by the Reb1p transcription factor, and the resulting activity increase is essential for the enzyme function in phospholipid synthesis. The DGK1-encoded DAG kinase activity causes the accumulation of phosphatidic acid at the nuclear/endoplasmicreticulum membrane when the phospholipid is not hydrolyzed by PAH1-encoded phosphatidic acid phosphatase Saccharomyces cerevisiae