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Literature summary extracted from

  • Sato, T.; Fujihashi, M.; Miyamoto, Y.; Kuwata, K.; Kusaka, E.; Fujita, H.; Miki, K.; Atomi, H.
    An uncharacterized member of the ribokinase family in Thermococcus kodakarensis exhibits myo-inositol kinase activity (2013), J. Biol. Chem., 288, 20856-20867.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.64 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL and Rosetta2(DE3)pLysS cells Thermococcus kodakarensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.64 sitting drop vapor diffusion method, usingv 18-23% (w/v) polyethylene glycol 3350 and 0.2 M NH4I Thermococcus kodakarensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.64 Ca2+ 49% residual activity at 3 mM Thermococcus kodakarensis
2.7.1.64 EDTA
-
 Thermococcus kodakarensis
2.7.1.64 Mn2+ 21% residual activity at 3 mM Thermococcus kodakarensis
2.7.1.64 Ni2+ 53% residual activity at 3 mM Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.64 0.36
-
 myo-inositol at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 53
-
 D-tagatose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 171
-
 D-Lyxose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 418
-
 D-xylose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 438
-
 D-talose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 524
-
 D-xylulose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 863
-
 D-allose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 951
-
 D-ribose at pH 7.5 and 85°C Thermococcus kodakarensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.64 Co2+ 133% activity at 3 mM Thermococcus kodakarensis
2.7.1.64 Zn2+ 125% activity at 3 mM Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.64 30300
-
-
 Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.64 ATP + myo-inositol Thermococcus kodakarensis
-
 ADP + 1D-myo-inositol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.64 Thermococcus kodakarensis Q5JDA3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.64 Resource Q column chromatography, Resource PHE hydrophobic column chromatography, and Superdex 200 gel filtration Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.64 0.063
-
 with meso-erythritol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 0.098
-
 with L-arabitol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 0.17
-
 with cis-inositol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 0.24
-
 with L-chiro-inositol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 0.39
-
 with adonitol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 0.5
-
 with D-arabitol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 1.06
-
 with myo-inositol 1-phosphate as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 1.91
-
 with allo-inositol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 17.64
-
 with scyllo-inositol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 34.92
-
 with D-chiro-inositol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 44.27
-
 with myo-inositol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.64 ATP + D-allose
-
 Thermococcus kodakarensis ?
-
 ?
2.7.1.64 ATP + D-lyxose
-
 Thermococcus kodakarensis ?
-
 ?
2.7.1.64 ATP + D-ribose
-
 Thermococcus kodakarensis ?
-
 ?
2.7.1.64 ATP + D-tagatose
-
 Thermococcus kodakarensis ?
-
 ?
2.7.1.64 ATP + D-talose
-
 Thermococcus kodakarensis ?
-
 ?
2.7.1.64 ATP + D-xylose
-
 Thermococcus kodakarensis ?
-
 ?
2.7.1.64 ATP + D-xylulose
-
 Thermococcus kodakarensis ?
-
 ?
2.7.1.64 ATP + myo-inositol
-
 Thermococcus kodakarensis ADP + 1D-myo-inositol 3-phosphate
-
 ?
2.7.1.64 additional information no activity with glycerol, DL-threitol, L-iditol, dulcitol, volemitol, D-lactitol monohydrate, maltitol, D-glucosamine, N-acetyl-D-glucosamine, maltose and lactose, D-erythrose, D-threose, D-erythrulose, L-arabinose, L-fructose, L-glucose, D-ribulose, D-galactose, fructose 1-phosphate, fructose 6-phosphate, glucose 1-phosphate, glucose 6-phosphate, ribose 1-phosphate, ribose 5-phosphate, tagatose 6-phosphate, adenosine, uridine, guanosine, cytidine, thymidine, and inosine Thermococcus kodakarensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.64 ? x * 30300, calculated from amino acid sequence Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.64 myo-inositol kinase
-
 Thermococcus kodakarensis
2.7.1.64 TK2285 protein
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.64 85
-
-
 Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.64 65 95 the enzyme is extremely thermostable with half-lives of 3 h at 95°C, 19 h at 85°C, 40 h at 75°C, and 88 h at 65°C Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.64 1.5
-
 D-tagatose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 3.9
-
 D-Lyxose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 5.1
-
 D-ribose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 5.6
-
 D-xylose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 11.6
-
 D-xylulose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 13.1
-
 D-talose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 18.3
-
 D-allose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 23.2
-
 myo-inositol at pH 7.5 and 85°C Thermococcus kodakarensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.64 0.005
-
 D-ribose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 0.013
-
 D-xylose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 0.021
-
 D-allose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 0.022
-
 D-xylulose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 0.023
-
 D-Lyxose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 0.028
-
 D-tagatose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 0.03
-
 D-talose at pH 7.5 and 85°C Thermococcus kodakarensis
2.7.1.64 64.9
-
 myo-inositol at pH 7.5 and 85°C Thermococcus kodakarensis