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Literature summary extracted from

  • Shigi, N.; Suzuki, T.; Terade, T.; Shirouzu, M.; Yokoyama, S.; Watanabe, K.
    Identification Temperature-dependent biosynthesis of 2-thioribothymidine of Thermus thermophilus tRNA (2006), J. Biol. Chem., 281, 2104-2113.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.15 expressed in Escherichia coli BL21 Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.15 ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor Thermus thermophilus
-
 AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor
-
 ?
2.8.1.15 ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor Thermus thermophilus DSM 7039
-
 AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor
-
 ?
2.8.1.15 ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.15 Thermus thermophilus Q72LF3
-
-
2.8.1.15 Thermus thermophilus DSM 7039 Q72LF3
-
-
2.8.1.15 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q72LF3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.15 ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor
-
 Thermus thermophilus AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor
-
 ?
2.8.1.15 ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor
-
 Thermus thermophilus DSM 7039 AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor
-
 ?
2.8.1.15 ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.1.15 TtuA
-
 Thermus thermophilus
2.8.1.15 TT_C0106
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.1.15 70
-
-
 Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.8.1.15 50 70 thiolation increases 7fold when the temperature rises from 50°C to 70°C Thermus thermophilus

Expression

EC Number Organism Comment Expression
2.8.1.15 Thermus thermophilus enzyme expression increases with increasing the temperature from 50°C to 80°C up

General Information

EC Number General Information Comment Organism
2.8.1.15 metabolism 2-Thioribothymidine (s2T) is a modified nucleoside of U, specifically found at position 54 of tRNAs from extreme thermophilic microorganisms. The function of the 2-thiocarbonyl group of s2T54 is thermostabilization of the three-dimensional structure of tRNA Thermus thermophilus
2.8.1.15 physiological function the sulfur atom of 2-thioribothymidine in tRNA is derived from cysteine or sulfate. Specific 2-thiolation of ribothymidine, at position 54, is observed in vitro, in the presence of ATP. The 2-thiolation reaction in vitro as well as expression of 2-thiolation enzymes in vivo show strong temperature dependence Thermus thermophilus