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Literature summary extracted from

  • Ouchane, S.; Steunou, A.S.; Picaud, M.; Astier, C.
    Aerobic and anaerobic Mg-protoporphyrin monomethyl ester cyclases in purple bacteria: a strategy adopted to bypass the repressive oxygen control system (2004), J. Biol. Chem., 279, 6385-6394.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.98.3 expression in Escherichia coli Rubrivivax gelatinosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.21.98.3 43000
-
-
 Rubrivivax gelatinosus
1.21.98.3 63000
-
-
 Rubrivivax gelatinosus
1.21.98.3 86000
-
 SDS-PAGE of truncated protein of 384 residues Rubrivivax gelatinosus

Organism

EC Number Organism UniProt Comment Textmining
1.21.98.3 Rubrivivax gelatinosus Q7X2C7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.98.3 recombinant protein, preparation is yellow-brown in color. Only a small fraction can be solubilized with urea (6 M), and most of the iron-sulfur cluster is degraded upon solubilization of inclusion bodies Rubrivivax gelatinosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.98.3 magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O
-
 Rubrivivax gelatinosus 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine overall reaction ?

Subunits

EC Number Subunits Comment Organism
1.21.98.3 dimer 2 * 63000, calculated, 2 * 43000, SDS-PAGE of truncated protein of 384 residues Rubrivivax gelatinosus

Synonyms

EC Number Synonyms Comment Organism
1.21.98.3 BChE
-
 Rubrivivax gelatinosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.21.98.3 [4Fe-4S]-center BchE sequence displays the conserved CXXXCXXC sequence essential for 4Fe-4S cluster formation and an active 4Fe-4S cluster is present in the protein. Isolated protein is yellow-brown in colour with an absorption peak around 410 nm Rubrivivax gelatinosus

Expression

EC Number Organism Comment Expression
1.21.98.3 Rubrivivax gelatinosus expression of the BchE gene is oxygen-independent additional information

General Information

EC Number General Information Comment Organism
1.21.98.3 physiological function a BchE mutant is photosynthesis-deficient, produces bacteriochlorophyll only under high oxygenation and accumulates Mg-protoporphyrin monomethyl ester under low oxygenation and anaerobiosis. A double knockout mutant lacking both Bche and aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase AcsF is devoid of photosystem and accumulates Mg-protoporphyrin monomethyl ester under both conditions indicating the involvement of the two enzymes at the same step of the biosynthesis pathway. AcsF acts strictly under high oxygenation conditions, whereas BchE is involved when the oxygen tension drops Rubrivivax gelatinosus