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Literature summary extracted from

  • Uwajima, T.; Shimizu, Y.; Terada, O.
    Glycerol oxidase, a novel copper hemoprotein from Aspergillus japonicus. Molecular and catalytic properties of the enzyme and its application to the analysis of serum triglycerides (1984), J. Biol. Chem., 259, 2748-2753.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.B4 Borate exposure of the enzyme to borate buffer of pH 10.0 results in activation of the enzyme Aspergillus japonicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.B4 2-mercaptoethanol 25% inhibition at 0.01 mM Aspergillus japonicus
1.1.3.B4 8-hydroxyquinoline 7.9% inhibition at 1 mM Aspergillus japonicus
1.1.3.B4 diethyldithiocarbamate 40.1% inhibition at 1 mM Aspergillus japonicus
1.1.3.B4 dimercapto-1-propanol 56.3% inhibition at 0.01 mM Aspergillus japonicus
1.1.3.B4 dithiothreitol 26.5% inhibition at 0.01 mM Aspergillus japonicus
1.1.3.B4 ethyl xanthate 27.1% inhibition at 0.1 mM Aspergillus japonicus
1.1.3.B4 L-ascorbic acid 31.2% inhibition at 0.1 mM Aspergillus japonicus
1.1.3.B4 L-cysteine 12.2% inhibition at 0.1 mM Aspergillus japonicus
1.1.3.B4 reduced glutathione 40.8% inhibition at 0.1 mM Aspergillus japonicus
1.1.3.B4 Salicylaldoxime 11.2% inhibition at 1 mM Aspergillus japonicus
1.1.3.B4 sodium sulfite 46.2% inhibition at 0.1 mM Aspergillus japonicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.B4 Co2+ contains 2 atoms of copper per mol of enzyme Aspergillus japonicus
1.1.3.B4 Mg2+ activates Aspergillus japonicus
1.1.3.B4 Mn2+ activates Aspergillus japonicus
1.1.3.B4 additional information the enzyme does not contain molybdenum and manganese Aspergillus japonicus
1.1.3.B4 Ni2+ activates Aspergillus japonicus
1.1.3.B4 Zn2+ contains 0.76 atoms of zinc per mol of enzyme. Zn2+ is the most effective cation with activation being 2.3fold at a concentration of 1.0 mM Aspergillus japonicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.B4 400000
-
 sedimentation equilibrium method Aspergillus japonicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.B4 glycerol + O2 Aspergillus japonicus
-
 glyceraldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.B4 Aspergillus japonicus
-
-
-
1.1.3.B4 Aspergillus japonicus AT 008
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.B4 DEAE-Sephadex A-25 column chromatography, hydroxylapatite column chromatography, and Sephadex G-200 gel filtration Aspergillus japonicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
1.1.3.B4 5°C, activated enzyme at pH 8.0-10.0, 1 week, no loss of activity Aspergillus japonicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.B4 1,3-butanediol + O2
-
 Aspergillus japonicus ?
-
 ?
1.1.3.B4 1,3-butanediol + O2
-
 Aspergillus japonicus AT 008 ?
-
 ?
1.1.3.B4 1,3-propanediol + O2
-
 Aspergillus japonicus ?
-
 ?
1.1.3.B4 1,4-butanediol + O2
-
 Aspergillus japonicus ?
-
 ?
1.1.3.B4 1,4-butanediol + O2
-
 Aspergillus japonicus AT 008 ?
-
 ?
1.1.3.B4 D-fructose + O2 slight oxidation activity Aspergillus japonicus ?
-
 ?
1.1.3.B4 D-fructose + O2 slight oxidation activity Aspergillus japonicus AT 008 ?
-
 ?
1.1.3.B4 D-galactose + O2 slight oxidation activity Aspergillus japonicus ?
-
 ?
1.1.3.B4 D-galactose + O2 slight oxidation activity Aspergillus japonicus AT 008 ?
-
 ?
1.1.3.B4 dihydroxyacetone + O2 58.8% activity compared to glycerol Aspergillus japonicus 3-hydroxy-2-oxopropanal + H2O2
-
 ?
1.1.3.B4 glycerol + O2
-
 Aspergillus japonicus glyceraldehyde + H2O2
-
 ?
1.1.3.B4 additional information no activity with glycerol 3-phosphate, methanol, ethanol, and polyvinyl alcohol Aspergillus japonicus ?
-
 ?
1.1.3.B4 additional information no activity with glycerol 3-phosphate, methanol, ethanol, and polyvinyl alcohol Aspergillus japonicus AT 008 ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.B4 45 50 the enzyme is stable up to 45°C and loses about 40% of activity at 50°C in 50 mM TES buffer, pH 7.0 Aspergillus japonicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.B4 7
-
-
 Aspergillus japonicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.B4 protoheme IX the enzyme contains 0.94 mol of heme per mol of enzyme Aspergillus japonicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.3.B4 0.012
-
 dimercapto-1-propanol at pH 7.0 and 37°C Aspergillus japonicus