Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sakdapetsiri, C.; Fukuta, Y.; Aramsirirujiwet, Y.; Shirasaka, N.; Kitpreechavanich, V.
    Antagonistic activity of endo-beta-1,3-glucanase from a novel isolate, Streptomyces sp. 9X166, against black rot in orchids (2016), J. Basic Microbiol., 56, 469-479.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.6 agriculture Streptomyces sp. 9X166 culture filtrate shows hydrolytic activity toward Phytophthora sp. Solutions with beta-1,3-glucanase activity are able to degrade both freeze-dried and living mycelium Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.6 EDTA 1 mM, 76% inhibition Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.6 additional information presence of divalent cations, except Pb2+, Hg2+, and Ag+, enhances the activity Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.6 80000
-
 gel filtration Streptomyces sp.
3.2.1.6 99000
-
 x * 99000, SDS-PAGE Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.6 Streptomyces sp.
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.6 58
-
 substrate laminarin, pH 6.0, 60°C Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.6 avicel + H2O 86% of the activity with laminarin Streptomyces sp. ?
-
 ?
3.2.1.6 carboxymethyl cellulose + H2O 49.6% of the activity with laminarin Streptomyces sp. ?
-
 ?
3.2.1.6 cellulose + H2O + 80% of the activity with laminarin Streptomyces sp. ?
-
 ?
3.2.1.6 curdlan + H2O + 54.6% of the activity with laminarin Streptomyces sp. ?
-
 ?
3.2.1.6 laminaribiose + H2O 39% of the activity with laminarin Streptomyces sp. 2 D-glucose
-
 ?
3.2.1.6 laminarin + H2O
-
 Streptomyces sp. D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.6 monomer x * 99000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.6 60
-
-
 Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.6 60
-
 almost complete inactivation above Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.6 6
-
-
 Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.6 4.2 8 60 min, 50°C, stable Streptomyces sp.