Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Moore, T.C.; Mera, P.E.; Escalante-Semerena, J.C.
    The EutT enzyme of Salmonella enterica is a unique ATP:Cob(I)alamin adenosyltransferase metalloprotein that requires ferrous ions for maximal activity (2014), J. Bacteriol., 196, 903-910.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.17 ATP the enzyme loses activity in the absence of ATP Salmonella enterica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.17 expressed in Escherichia coli BL21(DE3) cells Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.17 C79A the mutant shows 1 order of magnitude reduced catalytic efficiency compared to the wild type enzyme Salmonella enterica
2.5.1.17 C80A inactive Salmonella enterica
2.5.1.17 C83A the mutant shows 30fold reduced catalytic efficiency compared to the wild type enzyme Salmonella enterica
2.5.1.17 H67A the mutant shows 100fold reduced catalytic efficiency compared to the wild type enzyme Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.17 0.004
-
 cob(I)alamin at pH 7.0 and 37°C Salmonella enterica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.17 Fe2+ required for maximum activity Salmonella enterica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.17 30000
-
 2 * 30000, SDS-PAGE Salmonella enterica

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.17 Salmonella enterica
-
-
-
2.5.1.17 Salmonella enterica JE6583
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.5.1.17 the enzyme loses all activity upon exposure to air and H2O2 Salmonella enterica

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.17 HisTrap column chromatography and Sephadex G-25 gel filtration Salmonella enterica

Storage Stability

EC Number Storage Stability Organism
2.5.1.17 -20°C, in 50% glycerol, 6 months, no loss of activity Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.17 ATP + cob(I)alamin
-
 Salmonella enterica triphosphate + adenosylcob(I)alamine
-
 ?
2.5.1.17 ATP + cob(I)alamin
-
 Salmonella enterica JE6583 triphosphate + adenosylcob(I)alamine
-
 ?
2.5.1.17 ATP + cob(II)alamin
-
 Salmonella enterica triphosphate + adenosylcob(II)alamine
-
 ?
2.5.1.17 ATP + cob(II)alamin
-
 Salmonella enterica JE6583 triphosphate + adenosylcob(II)alamine
-
 ?
2.5.1.17 additional information the enzyme can use free dihydroflavins to drive the adenosylation of cob(II)alamin Salmonella enterica ?
-
 ?
2.5.1.17 additional information the enzyme can use free dihydroflavins to drive the adenosylation of cob(II)alamin Salmonella enterica JE6583 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.17 homodimer 2 * 30000, SDS-PAGE Salmonella enterica

Synonyms

EC Number Synonyms Comment Organism
2.5.1.17 ATP:cob(I)alamin adenosyltransferase
-
 Salmonella enterica
2.5.1.17 EutT
-
 Salmonella enterica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.17 0.06
-
 cob(I)alamin at pH 7.0 and 37°C Salmonella enterica