EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.229 | Aquincola tertiaricarbonis | G8FRC5 | - |
- |
1.14.13.229 | Aquincola tertiaricarbonis L108 | G8FRC5 | - |
- |
1.14.19.48 | Aquincola tertiaricarbonis | G8FRC5 | - |
- |
1.14.19.48 | Aquincola tertiaricarbonis L108 | G8FRC5 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.229 | tert-butyl alcohol + NADPH + H+ + O2 | - |
Aquincola tertiaricarbonis | 2-methylpropan-1,2-diol + NADP+ + 2 H2O | product is further metabolized to 2-hydroxyisobutanoate | ? | |
1.14.13.229 | tert-butyl alcohol + NADPH + H+ + O2 | - |
Aquincola tertiaricarbonis L108 | 2-methylpropan-1,2-diol + NADP+ + 2 H2O | product is further metabolized to 2-hydroxyisobutanoate | ? | |
1.14.19.48 | 3-methyl-3-pentanol + NADPH + H+ + O2 | - |
Aquincola tertiaricarbonis | 3-methyl-1-penten-3-ol + NADP+ + 2 H2O | - |
? | |
1.14.19.48 | 3-methyl-3-pentanol + NADPH + H+ + O2 | - |
Aquincola tertiaricarbonis L108 | 3-methyl-1-penten-3-ol + NADP+ + 2 H2O | - |
? | |
1.14.19.48 | additional information | MdpJ is not hydroxylating tert-amyl alcohol to diols but functions as a desaturase, resulting in the formation of the hemiterpene 3-methyl-1-buten-3-ol. The latter is further processed via the hemiterpenes prenol, prenal, and 3-methylcrotonic acid | Aquincola tertiaricarbonis | ? | - |
? | |
1.14.19.48 | additional information | MdpJ is not hydroxylating tert-amyl alcohol to diols but functions as a desaturase, resulting in the formation of the hemiterpene 3-methyl-1-buten-3-ol. The latter is further processed via the hemiterpenes prenol, prenal, and 3-methylcrotonic acid | Aquincola tertiaricarbonis L108 | ? | - |
? | |
1.14.19.48 | tert-amyl alcohol + NADPH + H+ + O2 | - |
Aquincola tertiaricarbonis | isoprenyl alcohol + NADP+ + 2 H2O | - |
? | |
1.14.19.48 | tert-amyl alcohol + NADPH + H+ + O2 | - |
Aquincola tertiaricarbonis L108 | isoprenyl alcohol + NADP+ + 2 H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.229 | MdpJ | - |
Aquincola tertiaricarbonis |
1.14.19.48 | MdpJ | - |
Aquincola tertiaricarbonis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.13.229 | physiological function | MdpJ knockout mutants are not able to grow on the tertiary alcohols tert-butanol and tert-amyl alcohol, whereas 2-methylpropan-1,2-diol, the putative product of tert-butanol hydroxylation by MdpJ catalysis can still be used as the sole source of energy and carbon. The postulated 2-methylpropan-1,2-diol intermediate 2-hydroxyisobutanoate is metabolized by wild-type and mutant strains at the same rates | Aquincola tertiaricarbonis |
1.14.19.48 | physiological function | MdpJ knockout mutants are not able to grow on the tertiary alcohols tert-butanol and tert-amyl alcohol, whereas 2-methylpropan-1,2-diol, the putative product of tert-butanol hydroxylation by MdpJ catalysis can still be used as the sole source of energy and carbon. The postulated 2-methylpropan-1,2-diol intermediate 2-hydroxyisobutanoate is metabolized by wild-type and mutant strains at the same rates. tert-amyl alcohol and hemiterpene degradation are linked with the catabolic route of leucine | Aquincola tertiaricarbonis |