Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Halsey, K.H.; Sayavedra-Soto, L.A.; Bottomley, P.J.; Arp, D.J.
    Site-directed amino acid substitutions in the hydroxylase alpha subunit of butane monooxygenase from Pseudomonas butanovora: Implications for substrates knocking at the gate (2006), J. Bacteriol., 188, 4962-4969.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.230 F321Y mutation in subunit BmoX towards soluble methane monooxygenase, no formation of butan-2-ol Thauera butanivorans
1.14.13.230 G113N mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 11.1 Thauera butanivorans
1.14.13.230 L279F mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 0.22 Thauera butanivorans
1.14.13.230 L287F mutation in subunit BmoX, loss of activity Thauera butanivorans
1.14.13.230 P179K mutation in subunit BmoX, loss of activity Thauera butanivorans
1.14.13.230 Q319G mutation in subunit BmoX, loss of activity Thauera butanivorans
1.14.13.230 Q320K mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 0.20 Thauera butanivorans
1.14.13.230 T148C mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 0.15 Thauera butanivorans
1.14.13.230 V181M mutation in subunit BmoX, loss of activity Thauera butanivorans

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.230 Thauera butanivorans Q8KQF0 and Q8KQE9 and Q8KQE7 Q8KQF0 i.e. alpha-subunit BmoX, Q8KQE9 i.e. beta-subunit BmoY, Q8KQE7 i.e. gamma-subunit BmoZ of hydrolase component, respectively
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.230 butane + NADH + H+ + O2
-
 Thauera butanivorans butan-1-ol + NAD+ + H2O formation of about 3.6% of butan-2-ol ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.230 BmoX
-
 Thauera butanivorans
1.14.13.230 BmoY
-
 Thauera butanivorans
1.14.13.230 BmoZ
-
 Thauera butanivorans

General Information

EC Number General Information Comment Organism
1.14.13.230 physiological function except for C1, strain BCP1 cells grow on the tested short-chain n-alkanes and show the lowest generation times with C3-C5 n-alkanes (2.7–2.8 h) Thauera butanivorans