BRENDA - Enzyme Database

Roles for the two 1-butanol dehydrogenases of Pseudomonas butanovora in butane and 1-butanol metabolism

Vangnai, A.; Sayavedra-Soto, L.; Arp, D.; J. Bacteriol. 184, 4343-4350 (2002)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.2.9
antimycin A
12% inhibition at 1 mM
Thauera butanivorans
1.1.2.9
additional information
not inhibited by cyanide (1 mM)
Thauera butanivorans
1.1.2.9
Salicylhydroxamic acid
7% inhibition at 3 mM
Thauera butanivorans
1.1.5.11
antimycin A
3% inhibition at 1 mM
Thauera butanivorans
1.1.5.11
additional information
not inhibited by cyanide (1 mM)
Thauera butanivorans
1.1.5.11
Salicylhydroxamic acid
5% inhibition at 3 mM
Thauera butanivorans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.5.11
0.0017
-
butan-1-ol
at pH 7.0 and 30C
Thauera butanivorans
1.1.5.11
0.369
-
Butyraldehyde
at pH 7.0 and 30C
Thauera butanivorans
1.1.5.11
0.662
-
2-butanol
at pH 7.0 and 30C
Thauera butanivorans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.2.9
periplasm
-
Thauera butanivorans
-
-
1.1.5.11
periplasm
-
Thauera butanivorans
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.2.9
butan-1-ol + ferricytochrome c
Thauera butanivorans
-
butanal + ferrocytochrome c + H+
-
-
?
1.1.2.9
butan-1-ol + ferricytochrome c
Thauera butanivorans ATCC 43655
-
butanal + ferrocytochrome c + H+
-
-
?
1.1.5.11
butan-1-ol + a quinone
Thauera butanivorans
-
butanal + a quinol
-
-
?
1.1.5.11
butan-1-ol + a quinone
Thauera butanivorans ATCC 43655
-
butanal + a quinol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.2.9
Thauera butanivorans
-
-
-
1.1.2.9
Thauera butanivorans ATCC 43655
-
-
-
1.1.5.11
Thauera butanivorans
-
-
-
1.1.5.11
Thauera butanivorans ATCC 43655
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.2.9
Q-Sepharose column chromatography and Superose 6 gel filtration
Thauera butanivorans
1.1.5.11
Q-Sepharose column chromatography and Superose 6 gel filtration
Thauera butanivorans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.2.9
butan-1-ol + ferricytochrome c
-
738514
Thauera butanivorans
butanal + ferrocytochrome c + H+
-
-
-
?
1.1.2.9
butan-1-ol + ferricytochrome c
-
738514
Thauera butanivorans ATCC 43655
butanal + ferrocytochrome c + H+
-
-
-
?
1.1.5.11
2-butanol + a quinone
-
738514
Thauera butanivorans
2-butanone + a quinol
-
-
-
?
1.1.5.11
2-butanol + a quinone
-
738514
Thauera butanivorans ATCC 43655
2-butanone + a quinol
-
-
-
?
1.1.5.11
butan-1-ol + a quinone
-
738514
Thauera butanivorans
butanal + a quinol
-
-
-
?
1.1.5.11
butan-1-ol + a quinone
-
738514
Thauera butanivorans ATCC 43655
butanal + a quinol
-
-
-
?
1.1.5.11
butyraldehyde + a quinol
-
738514
Thauera butanivorans
1-butanol + a quinone
-
-
-
?
1.1.5.11
butyraldehyde + a quinol
-
738514
Thauera butanivorans ATCC 43655
1-butanol + a quinone
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.2.9
heme c
-
Thauera butanivorans
1.1.2.9
pyrroloquinoline quinone
-
Thauera butanivorans
1.1.5.11
pyrroloquinoline quinone
-
Thauera butanivorans
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.2.9
heme c
-
Thauera butanivorans
1.1.2.9
pyrroloquinoline quinone
-
Thauera butanivorans
1.1.5.11
pyrroloquinoline quinone
-
Thauera butanivorans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.2.9
antimycin A
12% inhibition at 1 mM
Thauera butanivorans
1.1.2.9
additional information
not inhibited by cyanide (1 mM)
Thauera butanivorans
1.1.2.9
Salicylhydroxamic acid
7% inhibition at 3 mM
Thauera butanivorans
1.1.5.11
antimycin A
3% inhibition at 1 mM
Thauera butanivorans
1.1.5.11
additional information
not inhibited by cyanide (1 mM)
Thauera butanivorans
1.1.5.11
Salicylhydroxamic acid
5% inhibition at 3 mM
Thauera butanivorans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.5.11
0.0017
-
butan-1-ol
at pH 7.0 and 30C
Thauera butanivorans
1.1.5.11
0.369
-
Butyraldehyde
at pH 7.0 and 30C
Thauera butanivorans
1.1.5.11
0.662
-
2-butanol
at pH 7.0 and 30C
Thauera butanivorans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.2.9
periplasm
-
Thauera butanivorans
-
-
1.1.5.11
periplasm
-
Thauera butanivorans
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.2.9
butan-1-ol + ferricytochrome c
Thauera butanivorans
-
butanal + ferrocytochrome c + H+
-
-
?
1.1.2.9
butan-1-ol + ferricytochrome c
Thauera butanivorans ATCC 43655
-
butanal + ferrocytochrome c + H+
-
-
?
1.1.5.11
butan-1-ol + a quinone
Thauera butanivorans
-
butanal + a quinol
-
-
?
1.1.5.11
butan-1-ol + a quinone
Thauera butanivorans ATCC 43655
-
butanal + a quinol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.2.9
Q-Sepharose column chromatography and Superose 6 gel filtration
Thauera butanivorans
1.1.5.11
Q-Sepharose column chromatography and Superose 6 gel filtration
Thauera butanivorans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.2.9
butan-1-ol + ferricytochrome c
-
738514
Thauera butanivorans
butanal + ferrocytochrome c + H+
-
-
-
?
1.1.2.9
butan-1-ol + ferricytochrome c
-
738514
Thauera butanivorans ATCC 43655
butanal + ferrocytochrome c + H+
-
-
-
?
1.1.5.11
2-butanol + a quinone
-
738514
Thauera butanivorans
2-butanone + a quinol
-
-
-
?
1.1.5.11
2-butanol + a quinone
-
738514
Thauera butanivorans ATCC 43655
2-butanone + a quinol
-
-
-
?
1.1.5.11
butan-1-ol + a quinone
-
738514
Thauera butanivorans
butanal + a quinol
-
-
-
?
1.1.5.11
butan-1-ol + a quinone
-
738514
Thauera butanivorans ATCC 43655
butanal + a quinol
-
-
-
?
1.1.5.11
butyraldehyde + a quinol
-
738514
Thauera butanivorans
1-butanol + a quinone
-
-
-
?
1.1.5.11
butyraldehyde + a quinol
-
738514
Thauera butanivorans ATCC 43655
1-butanol + a quinone
-
-
-
?