EC Number | Application | Comment | Organism |
---|---|---|---|
4.4.1.34 | degradation | enzyme is involved in degradation of primary oxidation products of isoprene, cis-1,2-dichloroethanol, and trans-1,2-dichloroethanol | Rhodococcus sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.34 | 1,2-Epoxybutane | addition of low concentrations of 1,2-epoxybutane and 1,2-epoxyhexane strongly inhibits growth on isoprene but not growth on ethanol and irreversibly inhibits the epoxide-degrading activity | Rhodococcus sp. | |
4.4.1.34 | 1,2-epoxyhexane | addition of low concentrations of 1,2-epoxybutane and 1,2-epoxyhexane strongly inhibits growth on isoprene but not growth on ethanol and irreversibly inhibits the epoxide-degrading activity | Rhodococcus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.398 | 0.07 | - |
NAD+ | at pH 10.0 and 30°C | Rhodococcus sp. AD45 | |
1.1.1.398 | 1.4 | - |
1-hydroxy-2-glutathionyl-2-methyl-3-butene | at pH 10.0 and 30°C | Rhodococcus sp. AD45 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.398 | 25000 | - |
2 * 25000, SDS-PAGE | Rhodococcus sp. AD45 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.34 | (3R)-3,4-epoxy-3-methylbut-1-ene + glutathione | Rhodococcus sp. | - |
2-(glutathion-S-yl)-2-methylbut-3-en-1-ol | - |
? | |
4.4.1.34 | cis-1,2-dichloroepoxyethane + glutathione | Rhodococcus sp. | - |
1,2-dichloro-2-(glutathion-S-yl)-ethan-1-ol | - |
? | |
4.4.1.34 | epoxypropane + glutathione | Rhodococcus sp. | - |
2-(glutathion-S-yl)-propan-1-ol | - |
? | |
4.4.1.34 | trans-1,2-dichloroepoxyethane + glutathione | Rhodococcus sp. | - |
1,2-dichloro-2-(glutathion-S-yl)-ethan-1-ol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.398 | Rhodococcus sp. AD45 | - |
- |
- |
4.4.1.34 | Rhodococcus sp. | Q9RBP4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.398 | Blue Sepharose column chromatography, DE52 column chromatography, and Sephacryl S-300 gel filtration | Rhodococcus sp. AD45 |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.398 | 2.9 | - |
crude extract, at pH 10.0 and 30°C | Rhodococcus sp. AD45 |
1.1.1.398 | 18 | - |
after 6.2fold purification, at pH 10.0 and 30°C | Rhodococcus sp. AD45 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.398 | 1-hydroxy-2-glutathionyl-2-methyl-3-butene + NAD+ | - |
Rhodococcus sp. AD45 | N-(2-methyl-1-oxobut-3-en-2-yl)-L-gamma-glutamyl-L-cysteinylglycine + NADH + H+ | - |
? | |
1.1.1.398 | 2-glutathionyl-2-methyl-3-butenal + NAD+ + H2O | - |
Rhodococcus sp. AD45 | 2-glutathionyl-2-methyl-3-butenoic acid + NADH + H+ | - |
? | |
1.1.1.398 | additional information | no NADH formation is observed when GSH, methanol, ethanol, glycerol, 2-methylbutanol, 3-methyl-butanol, 1-hydroxy-3-methyl-3-butene, or 1,2-dihydroxy-2-methyl-3-butene are used as a substrate. The conjugates of GSH with 1,2-epoxybutane and 1,2-epoxyhexane are also not substrates | Rhodococcus sp. AD45 | ? | - |
? | |
4.4.1.34 | (3R)-3,4-epoxy-3-methylbut-1-ene + glutathione | - |
Rhodococcus sp. | 2-(glutathion-S-yl)-2-methylbut-3-en-1-ol | - |
? | |
4.4.1.34 | cis-1,2-dichloroepoxyethane + glutathione | - |
Rhodococcus sp. | 1,2-dichloro-2-(glutathion-S-yl)-ethan-1-ol | - |
? | |
4.4.1.34 | epoxypropane + glutathione | - |
Rhodococcus sp. | 2-(glutathion-S-yl)-propan-1-ol | - |
? | |
4.4.1.34 | trans-1,2-dichloroepoxyethane + glutathione | - |
Rhodococcus sp. | 1,2-dichloro-2-(glutathion-S-yl)-ethan-1-ol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.398 | homodimer | 2 * 25000, SDS-PAGE | Rhodococcus sp. AD45 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.4.1.34 | IsoI | - |
Rhodococcus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.398 | 9 | 10 | - |
Rhodococcus sp. AD45 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.398 | NAD+ | - |
Rhodococcus sp. AD45 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.4.1.34 | physiological function | the enzyme is a glutathione S-transferase involved in the metabolism of isoprene and able to detoxify reactive epoxides produced by monooxygenation of chlorinated ethenes | Rhodococcus sp. |