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Literature summary extracted from

  • Van Hylckama Vlieg, J.; Kingma, J.; Kruizinga, W.; Janssen, D.
    Purification of a glutathione S-transferase and a glutathione conjugate-specific dehydrogenase involved in isoprene metabolism in Rhodococcus sp. strain AD45 (1999), J. Bacteriol., 181, 2094-2101.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.4.1.34 degradation enzyme is involved in degradation of primary oxidation products of isoprene, cis-1,2-dichloroethanol, and trans-1,2-dichloroethanol Rhodococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.34 1,2-Epoxybutane addition of low concentrations of 1,2-epoxybutane and 1,2-epoxyhexane strongly inhibits growth on isoprene but not growth on ethanol and irreversibly inhibits the epoxide-degrading activity Rhodococcus sp.
4.4.1.34 1,2-epoxyhexane addition of low concentrations of 1,2-epoxybutane and 1,2-epoxyhexane strongly inhibits growth on isoprene but not growth on ethanol and irreversibly inhibits the epoxide-degrading activity Rhodococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.398 0.07
-
 NAD+ at pH 10.0 and 30°C Rhodococcus sp. AD45
1.1.1.398 1.4
-
 1-hydroxy-2-glutathionyl-2-methyl-3-butene at pH 10.0 and 30°C Rhodococcus sp. AD45

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.398 25000
-
 2 * 25000, SDS-PAGE Rhodococcus sp. AD45

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.34 (3R)-3,4-epoxy-3-methylbut-1-ene + glutathione Rhodococcus sp.
-
 2-(glutathion-S-yl)-2-methylbut-3-en-1-ol
-
 ?
4.4.1.34 cis-1,2-dichloroepoxyethane + glutathione Rhodococcus sp.
-
 1,2-dichloro-2-(glutathion-S-yl)-ethan-1-ol
-
 ?
4.4.1.34 epoxypropane + glutathione Rhodococcus sp.
-
 2-(glutathion-S-yl)-propan-1-ol
-
 ?
4.4.1.34 trans-1,2-dichloroepoxyethane + glutathione Rhodococcus sp.
-
 1,2-dichloro-2-(glutathion-S-yl)-ethan-1-ol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.398 Rhodococcus sp. AD45
-
-
-
4.4.1.34 Rhodococcus sp. Q9RBP4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.398 Blue Sepharose column chromatography, DE52 column chromatography, and Sephacryl S-300 gel filtration Rhodococcus sp. AD45

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.398 2.9
-
 crude extract, at pH 10.0 and 30°C Rhodococcus sp. AD45
1.1.1.398 18
-
 after 6.2fold purification, at pH 10.0 and 30°C Rhodococcus sp. AD45

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.398 1-hydroxy-2-glutathionyl-2-methyl-3-butene + NAD+
-
 Rhodococcus sp. AD45 N-(2-methyl-1-oxobut-3-en-2-yl)-L-gamma-glutamyl-L-cysteinylglycine + NADH + H+
-
 ?
1.1.1.398 2-glutathionyl-2-methyl-3-butenal + NAD+ + H2O
-
 Rhodococcus sp. AD45 2-glutathionyl-2-methyl-3-butenoic acid + NADH + H+
-
 ?
1.1.1.398 additional information no NADH formation is observed when GSH, methanol, ethanol, glycerol, 2-methylbutanol, 3-methyl-butanol, 1-hydroxy-3-methyl-3-butene, or 1,2-dihydroxy-2-methyl-3-butene are used as a substrate. The conjugates of GSH with 1,2-epoxybutane and 1,2-epoxyhexane are also not substrates Rhodococcus sp. AD45 ?
-
 ?
4.4.1.34 (3R)-3,4-epoxy-3-methylbut-1-ene + glutathione
-
 Rhodococcus sp. 2-(glutathion-S-yl)-2-methylbut-3-en-1-ol
-
 ?
4.4.1.34 cis-1,2-dichloroepoxyethane + glutathione
-
 Rhodococcus sp. 1,2-dichloro-2-(glutathion-S-yl)-ethan-1-ol
-
 ?
4.4.1.34 epoxypropane + glutathione
-
 Rhodococcus sp. 2-(glutathion-S-yl)-propan-1-ol
-
 ?
4.4.1.34 trans-1,2-dichloroepoxyethane + glutathione
-
 Rhodococcus sp. 1,2-dichloro-2-(glutathion-S-yl)-ethan-1-ol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.398 homodimer 2 * 25000, SDS-PAGE Rhodococcus sp. AD45

Synonyms

EC Number Synonyms Comment Organism
4.4.1.34 IsoI
-
 Rhodococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.398 9 10
-
 Rhodococcus sp. AD45

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.398 NAD+
-
 Rhodococcus sp. AD45

General Information

EC Number General Information Comment Organism
4.4.1.34 physiological function the enzyme is a glutathione S-transferase involved in the metabolism of isoprene and able to detoxify reactive epoxides produced by monooxygenation of chlorinated ethenes Rhodococcus sp.