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Literature summary extracted from

  • Hansson, M.; Hederstedt, L.
    Bacillus subtilis HemY is a peripheral membrane protein essential for protoheme IX synthesis which can oxidize coproporphyrinogen III and protoporphyrinogen IX (1994), J. Bacteriol., 176, 5962-5970.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.15 expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.15 protoporphyrinogen IX acts as a competitive inhibitor of the coproporphyrinogen III to coproporphyrin oxidase activity of HemY Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.3.15 membrane
-
 Bacillus subtilis 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.15 53000
-
 x * 53000, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.15 coproporphyrinogen III + O2 Bacillus subtilis 12fold higher catalytic efficiency with coproporphyrinogen III compared to protoporphyrinogen as substrate coproporphyrin III + H2O
-
 ?
1.3.3.15 coproporphyrinogen III + O2 Bacillus subtilis 3G18 12fold higher catalytic efficiency with coproporphyrinogen III compared to protoporphyrinogen as substrate coproporphyrin III + H2O
-
 ?
1.3.3.15 protoporphyrinogen IX + O2 Bacillus subtilis
-
 protoporphyrin IX + H2O
-
 ?
1.3.3.15 protoporphyrinogen IX + O2 Bacillus subtilis 3G18
-
 protoporphyrin IX + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.15 Bacillus subtilis
-
-
-
1.3.3.15 Bacillus subtilis 3G18
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.15 coproporphyrinogen III + O2 12fold higher catalytic efficiency with coproporphyrinogen III compared to protoporphyrinogen as substrate Bacillus subtilis coproporphyrin III + H2O
-
 ?
1.3.3.15 coproporphyrinogen III + O2 12fold higher catalytic efficiency with coproporphyrinogen III compared to protoporphyrinogen as substrate Bacillus subtilis 3G18 coproporphyrin III + H2O
-
 ?
1.3.3.15 additional information the enzyme does not oxidize uroporphyrinogen III Bacillus subtilis ?
-
 ?
1.3.3.15 additional information the enzyme does not oxidize uroporphyrinogen III Bacillus subtilis 3G18 ?
-
 ?
1.3.3.15 protoporphyrinogen IX + O2
-
 Bacillus subtilis protoporphyrin IX + H2O
-
 ?
1.3.3.15 protoporphyrinogen IX + O2
-
 Bacillus subtilis 3G18 protoporphyrin IX + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.15 ? x * 53000, SDS-PAGE Bacillus subtilis
1.3.3.15 ? x * 51203, calculated from amino acid sequence Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
1.3.3.15 hemY
-
 Bacillus subtilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.3.15 0.0003
-
 protoporphyrinogen IX at pH 7.2 and 30°C Bacillus subtilis