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Literature summary extracted from

  • Lerud, R.; Whiteley, H.
    Purification and properties of alpha-ketoglutarate reductase from Micrococcus aerogenes (1971), J. Bacteriol., 106, 571-577.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.399 additional information sodium acetate, acetyl phosphate, acetyl coenzyme A, glutamate, histidine, adenosine mono-, di-, and triphosphates, dithiothreitol, N-ethylmaleimide or iodoacetate at a concentration of 0.01-1 mM have no effect on the rate of the reaction Micrococcus aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.399 2-oxoglutarate inhibitor of the reverse reaction Micrococcus aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.399 0.12
-
 2-oxoglutarate at pH 6.5 and 25°C Micrococcus aerogenes
1.1.1.399 0.3
-
 NADH at pH 6.5 and 25°C Micrococcus aerogenes
1.1.1.399 1
-
 NAD+ at pH 8.5 and 25°C Micrococcus aerogenes
1.1.1.399 10
-
 (R)-2-hydroxyglutarate at pH 8.5 and 25°C Micrococcus aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.399 74000 78000 gel filtration Micrococcus aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.399 (R)-2-hydroxyglutarate + NAD+ Micrococcus aerogenes
-
 2-oxoglutarate + NADH + H+
-
 r
1.1.1.399 (R)-2-hydroxyglutarate + NAD+ Micrococcus aerogenes 228
-
 2-oxoglutarate + NADH + H+
-
 r
1.1.1.399 2-oxoglutarate + NADH + H+ Micrococcus aerogenes
-
 (R)-2-hydroxyglutarate + NAD+
-
 r
1.1.1.399 2-oxoglutarate + NADH + H+ Micrococcus aerogenes 228
-
 (R)-2-hydroxyglutarate + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.399 Micrococcus aerogenes
-
-
-
1.1.1.399 Micrococcus aerogenes 228
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.399 ammonium sulfate precipitation and hydroxylapatite column chromatography Micrococcus aerogenes

Storage Stability

EC Number Storage Stability Organism
1.1.1.399 -20°C, high protein concentrations (1 mg/ml or more), several months, no loss of activity Micrococcus aerogenes
1.1.1.399 0°C, high protein concentrations (1 mg/ml or more), several weeks, no loss of activity Micrococcus aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.399 (R)-2-hydroxyglutarate + NAD+
-
 Micrococcus aerogenes 2-oxoglutarate + NADH + H+
-
 r
1.1.1.399 (R)-2-hydroxyglutarate + NAD+
-
 Micrococcus aerogenes 228 2-oxoglutarate + NADH + H+
-
 r
1.1.1.399 2-oxoglutarate + NADH + H+
-
 Micrococcus aerogenes (R)-2-hydroxyglutarate + NAD+
-
 r
1.1.1.399 2-oxoglutarate + NADH + H+
-
 Micrococcus aerogenes 228 (R)-2-hydroxyglutarate + NAD+
-
 r
1.1.1.399 additional information NADPH is oxidized in the presence of 2-oxoglutarate. The rate of this reaction is 3 to 4% of that observed with NADH Micrococcus aerogenes ?
-
 ?
1.1.1.399 additional information oxaloacetate, pyruvate, 2-oxobutyrate, 2-oxoisovalerate, and glyoxalate are not reduced by the enzyme Micrococcus aerogenes ?
-
 ?
1.1.1.399 additional information NADPH is oxidized in the presence of 2-oxoglutarate. The rate of this reaction is 3 to 4% of that observed with NADH Micrococcus aerogenes 228 ?
-
 ?
1.1.1.399 additional information oxaloacetate, pyruvate, 2-oxobutyrate, 2-oxoisovalerate, and glyoxalate are not reduced by the enzyme Micrococcus aerogenes 228 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.399 homodimer 2 * 36000-40000, SDS-PAGE Micrococcus aerogenes

Synonyms

EC Number Synonyms Comment Organism
1.1.1.399 alpha-ketoglutarate reductase
-
 Micrococcus aerogenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.399 NADH
-
 Micrococcus aerogenes