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Literature summary extracted from

  • Liu, L.; Li, Y.; Wang, X.; Guo, W.
    A phosphoethanolamine transferase specific for the 4'-phosphate residue of Cronobacter sakazakii lipid A (2016), J. Appl. Microbiol., 121, 1444-1456.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.43 gene ESA_RS09200, encodes a phosphoethanolamine transferase that specifically adds a phosphoethanolamine to the 4'-phosphate residue of lipid A, but is not regulated by the PmrA-PmrB system, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain W3110 Cronobacter sakazakii

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.43 additional information construction of Cronobacter sakazakii mutant strains WLL001, a ESA_RS09200 deletion mutant, and of WLL002, WLL003 and WLL004, with mutationsof genes lpxE, ESA-RS16430, and ESA_RS16425, with or without additional deletion of ESA_RS09200. Analysis of mammalian cell invasion abilities of mutants using human enterocyte-like epithelial Caco-2 cells, overview Cronobacter sakazakii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.43 diacylphosphatidylethanolamine + lipid A Cronobacter sakazakii the phosphoethanolamine transferase is specific for the 4'-phosphate residue of Cronobacter sakazakii lipid A diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
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 ?
2.7.8.43 diacylphosphatidylethanolamine + lipid A Cronobacter sakazakii BAA894 the phosphoethanolamine transferase is specific for the 4'-phosphate residue of Cronobacter sakazakii lipid A diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.43 Cronobacter sakazakii A7MFE0 gene ESA_RS09200, formerly ESA_02008
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2.7.8.43 Cronobacter sakazakii BAA894 A7MFE0 gene ESA_RS09200, formerly ESA_02008
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.43 diacylphosphatidylethanolamine + lipid A the phosphoethanolamine transferase is specific for the 4'-phosphate residue of Cronobacter sakazakii lipid A Cronobacter sakazakii diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
-
 ?
2.7.8.43 diacylphosphatidylethanolamine + lipid A the phosphoethanolamine transferase is specific for the 4'-phosphate residue of Cronobacter sakazakii lipid A Cronobacter sakazakii BAA894 diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.43 PEA transferase
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 Cronobacter sakazakii

General Information

EC Number General Information Comment Organism
2.7.8.43 malfunction lipid A modification is observed in strain BAA894 when the 1-phosphate residue of lipid A is removed, but disappears when the 4'-phosphate residue of lipid A was removed. When gene ESA_RS16430, the orthologous gene of Escherichia coli pmrA, is deleted in Cronobacter sakazakii strain BAA894, this phosphoethanolamine modification of lipid A is still observed, suggesting that this modification is not regulated by the PmrA-PmrB system. Compared to the wild-type strain BAA894, the ESA_RS09200 deletion mutant shows decreased resistance to cationic antimicrobial peptides, increased recognition by TLR4/MD2, and decreased ability to invade and persist in mammalian cells. Phosphoethanolamine modification of lipid A reduces recognition and killing by the host innate immune system. Analysis of mammalian cell invasion abilities of mutants using human enterocyte-like epithelial Caco-2 cells, overview Cronobacter sakazakii
2.7.8.43 physiological function Cronobacter sakazakii modifies its lipid A structure through the enzyme to avoid recognition by the host immune cells. Gene ESA_RS09200, encodes a phosphoethanolamine transferase that specifically adds a phosphoethanolamine to the 4'-phosphate residue of lipid A, but is not regulated by the PmrA-PmrB system. The enzyme is active in cells grown at pH 5.0, not pH 7.0. Gene ESA_RS09200, but not ESA_RS16425, is required for phosphoethanolamine addition to the lipid A in strain BAA894 Cronobacter sakazakii