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Literature summary extracted from

  • Xie, J.; Zhao, D.; Zhao, L.; Pei, J.; Xiao, W.; Ding, G.; Wang, Z.; Xu, J.
    Characterization of a novel arabinose-tolerant alpha-L-arabinofuranosidase with high ginsenoside Rc to ginsenoside Rd bioconversion productivity (2016), J. Appl. Microbiol., 120, 647-660.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.195 synthesis in a reaction at 85°C and pH 5.0, 25 g/l of ginsenoside Rc is transformed into 21.8 g/l of ginsenoside Rd within 60 min, with a corresponding molar conversion of 99.4% and a high ginsenoside Rd productivity of 21800 mg/l/h Pseudothermotoga thermarum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 expression in Escherichia coli Pseudothermotoga thermarum
3.2.1.195 expression in Escherichia coli Pseudothermotoga thermarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 Hg2+ 1 mM, no residual activtiy Pseudothermotoga thermarum
3.2.1.55 additional information not inhibitory at 1 mM: EDTA Pseudothermotoga thermarum
3.2.1.195 Hg2+ 1 mM, no residual activtiy Pseudothermotoga thermarum
3.2.1.195 additional information not inhibitory at 1 mM: EDTA Pseudothermotoga thermarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.186
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 5.0, 95°C Pseudothermotoga thermarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 55000
-
 x * 55000, SDS-PAGE Pseudothermotoga thermarum
3.2.1.195 55000
-
 x * 55000, SDS-PAGE Pseudothermotoga thermarum

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.55 DMSO 10% v/v, 69% residual activity Pseudothermotoga thermarum
3.2.1.55 Ethanol 10% v/v, 71% residual activity Pseudothermotoga thermarum
3.2.1.55 Methanol 10% v/v, 56% residual activity Pseudothermotoga thermarum
3.2.1.195 DMSO 10% v/v, 69% residual activity Pseudothermotoga thermarum
3.2.1.195 Ethanol 10% v/v, 71% residual activity Pseudothermotoga thermarum
3.2.1.195 Methanol 10% v/v, 56% residual activity Pseudothermotoga thermarum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Pseudothermotoga thermarum F7YTI3
-
-
3.2.1.55 Pseudothermotoga thermarum DSM 5069 F7YTI3
-
-
3.2.1.195 Pseudothermotoga thermarum F7YTI3
-
-
3.2.1.195 Pseudothermotoga thermarum DSM 5069 F7YTI3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Pseudothermotoga thermarum 4-nitrophenol + L-arabinose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Pseudothermotoga thermarum DSM 5069 4-nitrophenol + L-arabinose
-
 ?
3.2.1.55 ginsenoside Rc + H2O
-
 Pseudothermotoga thermarum ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.55 ginsenoside Rc + H2O
-
 Pseudothermotoga thermarum DSM 5069 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.195 ginsenoside Rc + H2O
-
 Pseudothermotoga thermarum ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.195 ginsenoside Rc + H2O
-
 Pseudothermotoga thermarum DSM 5069 ginsenoside Rd + L-arabinofuranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 ? x * 55000, SDS-PAGE Pseudothermotoga thermarum
3.2.1.195 ? x * 55000, SDS-PAGE Pseudothermotoga thermarum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 Theth_1120
-
 Pseudothermotoga thermarum
3.2.1.195 Theth_1120
-
 Pseudothermotoga thermarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 95
-
-
 Pseudothermotoga thermarum
3.2.1.195 95
-
-
 Pseudothermotoga thermarum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.55 75 100 more than 50% of maximum activtiy Pseudothermotoga thermarum
3.2.1.195 75 100 more than 50% of maximum activtiy Pseudothermotoga thermarum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.55 151
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 5.0, 95°C Pseudothermotoga thermarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 5
-
-
 Pseudothermotoga thermarum
3.2.1.195 5
-
-
 Pseudothermotoga thermarum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.55 4 7.5 stable Pseudothermotoga thermarum
3.2.1.195 4 7.5 stable Pseudothermotoga thermarum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.55 811
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 5.0, 95°C Pseudothermotoga thermarum