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Literature summary extracted from

  • So, J.; Lim, Y.; Kim, S.; Kim, H.; Rhee, I.
    Co-expression of gamma-aminobutyrate aminotransferase and succinic semialdehyde dehydrogenase genes for the enzymatic analysis of gamma-aminobutyric acid in Escherichia coli (2013), J. Appl. Biol. Chem., 56, 89-93.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.19 expression in Escherichia coli Pseudomonas fluorescens

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.19 synthesis simultaneous expression of gamma-aminobutyric acid aminotransferase gabT and succinic semialdehyde dehydrogenase gabD genes in Escherichia coli. The mixture of both enzymes, called GABase, is the key enzyme for the enzymatic analysis of GABA Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.19 Pseudomonas fluorescens
-
-
-
2.6.1.19 Pseudomonas fluorescens KCCM 12537
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.19 4-aminobutanoate + pyruvate
-
 Pseudomonas fluorescens 4-oxobutanoate + L-alanine
-
 ?
2.6.1.19 4-aminobutanoate + pyruvate
-
 Pseudomonas fluorescens KCCM 12537 4-oxobutanoate + L-alanine
-
 ?
2.6.1.19 5-aminovaleric acid + pyruvate
-
 Pseudomonas fluorescens 5-oxopentanoate + L-alanine 47.7% of the activity with 4-aminobutanoate ?
2.6.1.19 5-aminovaleric acid + pyruvate
-
 Pseudomonas fluorescens KCCM 12537 5-oxopentanoate + L-alanine 47.7% of the activity with 4-aminobutanoate ?
2.6.1.19 additional information GABase activity against beta-alanine, epsilon-amino-n-caproic acid, L-ornithine, L-lysine, and L-aspartic acid is between 0.3 to 2.3% of the activity against 4-aminobutanoate Pseudomonas fluorescens ?
-
 ?
2.6.1.19 additional information GABase activity against beta-alanine, epsilon-amino-n-caproic acid, L-ornithine, L-lysine, and L-aspartic acid is between 0.3 to 2.3% of the activity against 4-aminobutanoate Pseudomonas fluorescens KCCM 12537 ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.19 25 35 so-called GABase, mixture of gamma-aminobutyric acid aminotransferase gabT and succinic semialdehyde dehydrogenase gabD, pH 8.6, 30 min, more than 80% of initial activity Pseudomonas fluorescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.19 6 9 so-called GABase, mixture of gamma-aminobutyric acid aminotransferase gabT and succinic semialdehyde dehydrogenase gabD, more than 75% of initial activity for 24 h at 4°C Pseudomonas fluorescens