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Literature summary extracted from

  • Venkatareddy, M.; Verma, R.; Kalinowski, A.; Patel, S.R.; Shisheva, A.; Garg, P.
    Distinct requirements for vacuolar protein sorting 34 downstream effector phosphatidylinositol 3-phosphate 5-kinase in podocytes versus proximal tubular cells (2016), J. Am. Soc. Nephrol., 27, 2702-2719.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.137 vacuole
-
 Homo sapiens 5773
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.137 Mg2+ required Homo sapiens
2.7.1.150 Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.137 ATP + 1-phosphatidyl-1D-myo-inositol Homo sapiens
-
 ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.1.150 ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate Mus musculus
-
 ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.137 Homo sapiens Q8NEB9
-
-
2.7.1.150 Mus musculus Q9Z1T6 gene PIKfyve
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.137 podocyte
-
 Homo sapiens
-
2.7.1.150 podocyte
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.137 ATP + 1-phosphatidyl-1D-myo-inositol
-
 Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.1.150 ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 Mus musculus ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.137 class 3 phosphatidylinositol 3-kinase vacuolar protein sorting 34
-
 Homo sapiens
2.7.1.137 PtdIns 3-kinase vacuolar protein sorting 34
-
 Homo sapiens
2.7.1.137 VPS34
-
 Homo sapiens
2.7.1.150 PIKfyve
-
 Mus musculus
2.7.1.150 PtdIns 3-phosphate 5-kinase
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.137 ATP
-
 Homo sapiens
2.7.1.150 ATP
-
 Mus musculus

General Information

EC Number General Information Comment Organism
2.7.1.137 malfunction deletion of a key endosomal trafficking regulator, the class 3 phosphatidylinositol (PtdIns) 3-kinase vacuolar protein sorting 34 (Vps34), in podocytes results in aberrant endosomal membrane morphology and podocyte dysfunction. The vacuolation phenotype in cultured Vps34-deficient podocytes is caused by the absence of a substrate for the Vps34 downstream effector PtdIns 3-phosphate 5-kinase, which phosphorylates Vps34-generated PtdIns(3)P to produce PtdIns (3,5)P2 Homo sapiens
2.7.1.150 malfunction the vacuolation phenotype in cultured Vps34 (EC 2.7.1.137)-deficient podocytes is caused by the absence of a substrate for the Vps34 downstream effector PtdIns 3-phosphate 5-kinase, which phosphorylates Vps34-generated 1-phosphatidyl-1D-myo-inositol 3-phosphate to produce + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate. PtdIns 3-phosphate 5-kinase perturbation and 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate reduction result in massive membrane vacuolation along the endosomal system. Genetic deletion of the enzyme in endocytically active proximal tubular cells results in the development of large cytoplasmic vacuoles caused by arrested endocytic traffic progression at a late-endosome stage, while deletion of the enzyme in glomerular podocytes does not significantly alter the endosomal morphology, even in age 18-month-old mice Mus musculus