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Literature summary extracted from

  • Cao, L.; Chen, R.; Xie, W.; Liu, Y.
    Enhancing the thermostability of feruloyl esterase EstF27 by directed evolution and the underlying structural basis (2015), J. Agric. Food Chem., 63, 8225-8233.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.73 T27I/S84L/V161I/G243C mutant displays significantly improved thermostability uncultured bacterium
3.1.1.73 T27I/S84L/V161I/H195L/G243C/A259V mutant displays significantly improved thermostability uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.36
-
 4-nitrophenyl ferulate mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 40°C uncultured bacterium
3.1.1.73 0.42
-
 4-nitrophenyl ferulate mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 65°C uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 uncultured bacterium E7DJY5
-
-

Storage Stability

EC Number Storage Stability Organism
3.1.1.73 25°C, storage for 150 days, the activity of mutant T27I/S84L/V161I/H195L/G243C/A259V remains almost constant (100.5%), whereas that of the wild-type is only 21.2% uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 4-nitrophenyl ferulate + H2O
-
 uncultured bacterium ferulic acid + 4-nitrophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 EstF27
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 40
-
 wild-type uncultured bacterium
3.1.1.73 60
-
 mutant T27I/S84L/V161I/G243C uncultured bacterium
3.1.1.73 65
-
 mutant T27I/S84L/V161I/H195L/G243C/A259V uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 55
-
 half-life of wild-type 0.5 h, mutant T27I/S84L/V161I/G243C 70 h, mutant T27I/S84L/V161I/H195L/G243C/A259V 1680 h, respectively uncultured bacterium
3.1.1.73 65
-
 half-life of mutant T27I/S84L/V161I/G243C 4 h, mutant T27I/S84L/V161I/H195L/G243C/A259V 140 h, respectively uncultured bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 18.9
-
 4-nitrophenyl ferulate mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 40°C uncultured bacterium
3.1.1.73 32.2
-
 4-nitrophenyl ferulate mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 65°C uncultured bacterium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.73 52.4
-
 4-nitrophenyl ferulate mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 40°C uncultured bacterium
3.1.1.73 76.6
-
 4-nitrophenyl ferulate mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 65°C uncultured bacterium