Literature summary extracted from
Tan, X.; Hu, X.M.; Zhong, X.W.; Chen, Q.M.; Xia, Q.Y.; Zhao, P.
Antenna-specific glutathione S-transferase in male silkmoth Bombyx mori (2014), Int. J. Mol. Sci., 15, 7429-7443.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.5.1.18 |
expressed in Escherichia coli Rosetta (DE3) cells |
Bombyx mori |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.5.1.18 |
26000 |
- |
x * 26000, SDS-PAGE |
Bombyx mori |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.5.1.18 |
Bombyx mori |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.5.1.18 |
nickel-chelating column chromatography |
Bombyx mori |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
2.5.1.18 |
antenna |
the enzyme localizes in the sensillum of male silkmoth antennae |
Bombyx mori |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.5.1.18 |
1-chloro-2,4-dinitrobenzene + glutathione |
- |
Bombyx mori |
S-(2,4-dinitrophenyl)glutathione + HCl |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.5.1.18 |
? |
x * 26000, SDS-PAGE |
Bombyx mori |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.5.1.18 |
glutathione S-transferase |
- |
Bombyx mori |
2.5.1.18 |
GSTD4 |
- |
Bombyx mori |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.5.1.18 |
25 |
- |
above 40° the enzyme retains about 20% of its 25°C activity |
Bombyx mori |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
2.5.1.18 |
25 |
40 |
the enzyme is stable up to 35°C |
Bombyx mori |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.5.1.18 |
6 |
6.5 |
- |
Bombyx mori |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
2.5.1.18 |
physiological function |
the enzyme is involved in the detoxification of harmful chemicals |
Bombyx mori |