EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.9 | guanidine hydrochloride | guanidine hydrochloride-induced unfolding of thymidylate kinase is noncooperative and influences the functional properties of the enzyme much less than their Cm values. Complete inhibition at 0.4 M | Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | Urea | urea-induced unfolding of thymidylate kinase is noncooperative and influences the functional properties of the enzyme much less than their Cm values. Complete inhibition at 1.0 M | Wolbachia endosymbiont of Brugia malayi |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.9 | Wolbachia endosymbiont of Brugia malayi | Q5GTD5 | - |
- |
2.7.4.9 | Wolbachia endosymbiont of Brugia malayi TRS | Q5GTD5 | - |
- |
EC Number | Renatured (Comment) | Organism |
---|---|---|
2.7.4.9 | guanidine hydrochloride and urea-induced unfolding of thymidylate kinase is noncooperative and influences the functional properties of the enzyme much less than their Cm values. Thymidylate kinase is more prone to ionic perturbation | Wolbachia endosymbiont of Brugia malayi |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.4.9 | More | the dimeric assembly of thymidylate kinase is an absolute requirement for enzymatic activity and any subtle change in dimeric conformation due to denaturation leads to loss of enzymatic activity | Wolbachia endosymbiont of Brugia malayi |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.7.4.9 | 3 | 4 | about 100-70% loss of structure | Wolbachia endosymbiont of Brugia malayi |
2.7.4.9 | 5 | 8 | structurally and functionally stable | Wolbachia endosymbiont of Brugia malayi |
2.7.4.9 | 9 | 10 | about 30-40% loss of structure | Wolbachia endosymbiont of Brugia malayi |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.7.4.9 | 155 | - |
pH 7.4, 37°C | Wolbachia endosymbiont of Brugia malayi | guanidine hydrochloride | |
2.7.4.9 | 600 | - |
pH 7.4, 37°C | Wolbachia endosymbiont of Brugia malayi | Urea |