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Literature summary extracted from

  • Cai, Y.; Lee, J.; Wang, W.; Yang, J.M.; Qian, G.Y.
    Effect of Cd2+ on muscle type of creatine kinase: inhibition kinetics integrating computational simulations (2016), Int. J. Biol. Macromol., 83, 233-241.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.3.2 Cd2+ Cd2+ conspicuously inactivates the activity of the muscle-type enzyme in a first-order kinetic process and exhibits non-competitive inhibition with creatine and ATP. Cd2+ induces tertiary structure changes in enzyme PSCKM with exposure of hydrophobic surfaces. The addition of osmolytes, such as glycine and proline, partially reactivates the enzyme. Molecular dynamics and docking simulations between PSCKM and Cd2+ show that Cd2+ blocks the entrance of ATP to the active site of the enzyme, computational modeling, overview Pelodiscus sinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.2 0.729
-
ATP pH 9.0, 20°C Pelodiscus sinensis
2.7.3.2 26.38
-
Creatine pH 9.0, 20°C Pelodiscus sinensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.3.2 Mg2+ required Pelodiscus sinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.3.2 ATP + creatine Pelodiscus sinensis
-
ADP + phosphocreatine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Pelodiscus sinensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.2 native enzyme from muscle by anion exchange chromatography and gel filtration Pelodiscus sinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.2 muscle muscle-type creatine kinase Pelodiscus sinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ATP + creatine
-
Pelodiscus sinensis ADP + phosphocreatine
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.3.2 PSCKM
-
Pelodiscus sinensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.3.2 20
-
assay at Pelodiscus sinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.3.2 9
-
assay at Pelodiscus sinensis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.3.2 ATP
-
Pelodiscus sinensis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.3.2 additional information
-
additional information inactivation rate constants for enzyme PSCKM by Cd2+., overview Pelodiscus sinensis
2.7.3.2 0.423
-
Cd2+ versus ATP, pH 9.0, 20°C Pelodiscus sinensis
2.7.3.2 0.549
-
Cd2+ versus creatine, pH 9.0, 20°C Pelodiscus sinensis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.3.2 0.062
-
pH 9.0, 20°C Pelodiscus sinensis Cd2+