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Literature summary extracted from

  • Kumari, U.; Vishwakarma, R.K.; Sonawane, P.; Abbassi, S.; Khan, B.M.
    Biochemical characterization of recombinant mevalonate kinase from Bacopa monniera (2015), Int. J. Biol. Macromol., 72, 776-783.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.36 expressed in Escherichia coli BL21(DE3) cells Bacopa monnieri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.36 0.1474
-
 ATP at pH 7.0 and 30°C Bacopa monnieri
2.7.1.36 0.3319
-
 (R)-mevalonate at pH 7.0 and 30°C Bacopa monnieri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.36 Mg2+ required for full activity, not replaceable by other divalent ions Bacopa monnieri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.36 46000
-
 1 * 46000, SDS-PAGE Bacopa monnieri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate Bacopa monnieri
-
 ADP + (R)-5-phosphomevalonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Bacopa monnieri M1F2U2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.36 Ni-NTA column chromatography and gel filtration Bacopa monnieri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate
-
 Bacopa monnieri ADP + (R)-5-phosphomevalonate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.36 monomer 1 * 46000, SDS-PAGE Bacopa monnieri

Synonyms

EC Number Synonyms Comment Organism
2.7.1.36 ATP: mevalonate 5-phosphotransferase
-
 Bacopa monnieri
2.7.1.36 mevalonate phosphokinase
-
 Bacopa monnieri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.36 30
-
-
 Bacopa monnieri

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.36 25 35 more than 50% activity between 25°C and 35°C Bacopa monnieri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.36 20 30 the enzyme is stable at 20-25°C for 80 min and at optimum temperature 30°C for 40 min Bacopa monnieri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.36 143.8
-
 (R)-mevalonate at pH 7.0 and 30°C Bacopa monnieri
2.7.1.36 150.9
-
 ATP at pH 7.0 and 30°C Bacopa monnieri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.36 7
-
-
 Bacopa monnieri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.36 6.5 9.5 more than 50% activity between pH 6.5 and 9.5 Bacopa monnieri

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.36 7 8 the enzyme is most stable at pH 7.0-8.0 at 25°C with half-life of 27-49 h Bacopa monnieri

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.36 433.3
-
 (R)-mevalonate at pH 7.0 and 30°C Bacopa monnieri
2.7.1.36 1023
-
 ATP at pH 7.0 and 30°C Bacopa monnieri