EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.4.6 | gene ndk, sequence comparison | Halomonas sp. |
2.7.4.6 | gene ndk, sequence comparison, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21Star (DE3) , subcloning in Escherichia coli strains JM109 and DH5alpha | Chromohalobacter salexigens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.4.6 | C139S | site-directed mutagenesis, the mutation in the dimeric nucleoside diphosphate kinase generates a catalytically competent enzyme monomer | Halomonas sp. |
2.7.4.6 | C139S | site-directed mutagenesis, the mutation in the dimeric nucleoside diphosphate kinase generates a catalytically competent enzyme monomer. Substitution of Cys139 for Ser causes dissociation of dimeric CsNDK into monomer in Tris buffer, and the mutant CsNDK becomes more susceptible to endproteinase GluC cleavage, which is suppressed by an NDK substrate, ATP | Chromohalobacter salexigens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.6 | Chromohalobacter salexigens | Q1QTL2 | gene ndk | - |
2.7.4.6 | Chromohalobacter salexigens DSM 3043 | Q1QTL2 | gene ndk | - |
2.7.4.6 | Halomonas sp. | Q83WH5 | - |
- |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.4.6 | dimer | wild-type enzyme | Chromohalobacter salexigens |
2.7.4.6 | dimer | wild-type enzyme | Halomonas sp. |
2.7.4.6 | monomer | enzyme mutant C139S | Chromohalobacter salexigens |
2.7.4.6 | monomer | enzyme mutant C139S | Halomonas sp. |
2.7.4.6 | More | three-dimensional structure structure modeling | Chromohalobacter salexigens |
2.7.4.6 | More | three-dimensional structure structure modeling | Halomonas sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.4.6 | NDK | - |
Chromohalobacter salexigens |
2.7.4.6 | NDK | - |
Halomonas sp. |
2.7.4.6 | nucleoside diphosphate kinase | - |
Chromohalobacter salexigens |
2.7.4.6 | nucleoside diphosphate kinase | - |
Halomonas sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.4.6 | evolution | enzymes from Halomonas sp strain 593 and Chromohalobacter salexigens strain DSM3043 show very high sequence homology to each other, in both enzymes, residue C139 is conserved | Chromohalobacter salexigens |
2.7.4.6 | evolution | enzymes from Halomonas sp strain 593 and Chromohalobacter salexigens strain DSM3043 show very high sequence homology to each other, in both enzymes, residue C139 is conserved | Halomonas sp. |