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Literature summary extracted from

  • Bashari, M.; Jin, Z.; Wang, J.; Zhan, X.
    A novel technique to improve the biodegradation efficiency of dextranase enzyme using the synergistic effects of ultrasound combined with microwave shock (2016), Innov. Food Sci. Emerg. Technol., 35, 125-132.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.11 degradation the hydrolysis of dextran under ultrasound/microwave irradiation shock treatment is significantly higher than those performed under ultrasound, microwave irradiation shock and conventional thermal incubation at all conditions studied. The maximum hydrolysis rate is observed when ultrasound/microwave irradiation shock (ultrasound of 50 W combined with microwave irradiation shock of 60 W at a sock rate of 20 s/min for 25 min) is used in which the dextran hydrolysis increases by 171.13% compared with routine conventional heating. Vmax and KM values of dextranase under ultrasound/microwave irradiation shock treatment are higher than those under ultrasound, microwave irradiation shock and conventional thermal incubation Chaetomium erraticum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.11
-
 Chaetomium erraticum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.11 71.4
-
 Dextran microwave irradiation shock treatment, pH 5.4, 40°C Chaetomium erraticum
3.2.1.11 75.6
-
 Dextran pH 5.4, 40°C Chaetomium erraticum
3.2.1.11 84.6
-
 Dextran ultrasound treatment, pH 5.4, 40°C Chaetomium erraticum
3.2.1.11 87.1
-
 Dextran ultrasound/microwave irradiation shock treatment, pH 5.4, 40°C Chaetomium erraticum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.11 Chaetomium erraticum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.11 dextran + H2O
-
 Chaetomium erraticum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.11 60
-
 optimum temperature under ultrasound/microwave irradiation shock treatment Chaetomium erraticum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.11 5.3
-
 Dextran microwave irradiation shock treatment, pH 5.4, 40°C Chaetomium erraticum
3.2.1.11 5.4
-
 Dextran pH 5.4, 40°C Chaetomium erraticum
3.2.1.11 6.4
-
 Dextran ultrasound treatment, pH 5.4, 40°C Chaetomium erraticum
3.2.1.11 6.7
-
 Dextran ultrasound/microwave irradiation shock treatment, pH 5.4, 40°C Chaetomium erraticum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.11 72
-
 Dextran pH 5.4, 40°C Chaetomium erraticum
3.2.1.11 74.5
-
 Dextran microwave irradiation shock treatment, pH 5.4, 40°C Chaetomium erraticum
3.2.1.11 75.15
-
 Dextran ultrasound treatment, pH 5.4, 40°C Chaetomium erraticum
3.2.1.11 76.78
-
 Dextran ultrasound/microwave irradiation shock treatment, pH 5.4, 40°C Chaetomium erraticum